Protective effect of uric acid against 6-OHDA-induced injury in SH-SY5Y cells
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KeywordsWestern Blot Cell Viability Blot Analysis Beneficial Effect Protective Effect
To investigate the effect of uric acid (UA) on 6-hydroxydopamine (6-OHDA) -induced injury in SH-SY5Y cells.
The cell viability was measured by the MTT reduction assay. The cell apoptosis was assessed by Hoechst 33342 staining with fluorescence microscopy. The phophorylation of Akt and GSK-3β(ser9) was determined by Western blot analysis.
Treatment with 6-OHDA at 50µM for 12 h significantly decreased the viability of SH-SY5Y cells. Pretreatment with UA (200-400µM, 0.5h) prior to 6-OHDA treatment markedly increased the cell viability of SH-SY5Y cells, as compared to that of 6-OHDA-treated group. The beneficial effects of UA against 6-OHDA-induced apoptosis were also confirmed by Hoechst 33342 staining assay. Moreover, 6-OHDA decreased the Akt activity and increased the GSK-3β activity, which could be blocked by UA (200-400µM) pretreatment.
These data suggest that 6-OHDA-induced cell injury was attenuated by UA. The underlying mechanisms may involve the up-regulation of Akt and the reduction of GSK-3β activity.
Contract grant sponsor: The work was supported by the Suzhou Foundation for Development of Science, Technology (200815404), Jiangsu Ordinary University Science Research Project (08KJB320012) and Natural Science Foundation of Jiangsu Province, China (BK2010229).
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