Protective effect of uric acid against 6-OHDA-induced injury in SH-SY5Y cells
- 1.2k Downloads
KeywordsWestern Blot Cell Viability Blot Analysis Beneficial Effect Protective Effect
To investigate the effect of uric acid (UA) on 6-hydroxydopamine (6-OHDA) -induced injury in SH-SY5Y cells.
The cell viability was measured by the MTT reduction assay. The cell apoptosis was assessed by Hoechst 33342 staining with fluorescence microscopy. The phophorylation of Akt and GSK-3β(ser9) was determined by Western blot analysis.
Treatment with 6-OHDA at 50µM for 12 h significantly decreased the viability of SH-SY5Y cells. Pretreatment with UA (200-400µM, 0.5h) prior to 6-OHDA treatment markedly increased the cell viability of SH-SY5Y cells, as compared to that of 6-OHDA-treated group. The beneficial effects of UA against 6-OHDA-induced apoptosis were also confirmed by Hoechst 33342 staining assay. Moreover, 6-OHDA decreased the Akt activity and increased the GSK-3β activity, which could be blocked by UA (200-400µM) pretreatment.
These data suggest that 6-OHDA-induced cell injury was attenuated by UA. The underlying mechanisms may involve the up-regulation of Akt and the reduction of GSK-3β activity.
Contract grant sponsor: The work was supported by the Suzhou Foundation for Development of Science, Technology (200815404), Jiangsu Ordinary University Science Research Project (08KJB320012) and Natural Science Foundation of Jiangsu Province, China (BK2010229).
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.