Pregnancy following homologous prepubertal ovarian transplantation in the dog
- 4.1k Downloads
In several canine models of hereditary human disease the homozygote dogs die prior to puberty, or have substantially reduced fertility. To create a clinically healthy animal that can be bred, but can also transmit the gene of interest, a model of homologous ovarian transplantation in prepubertal dogs was developed. Six dog leukocyte antigen (DLA) identical littermates underwent transplantation of ovarian cortical strips (n = 2) or the entire ovary (n = 4). Immunosuppression was maintained with cyclosporine and MMF in the immediate post-operative period and cyclosporine alone thereafter. All 6 dogs entered puberty and normal semiannual estrus cycles as demonstrated by both physical changes and increasing serum progesterone. Four dogs were bred to a proven stud male, and one became pregnant. Three viable fetuses with observable heartbeats were detected on ultrasound examination. Although the dog eventually aborted the litter, this work represents the first pregnancy achieved following a prepubertal ovarian transplant in the dog.
List of abbreviations
dog leukocyte antigen
This work was supported by NIH 5R24HD047142 to Dr. Pullium. The authors thank Dr. Richard Fayrer-Hoskins for helpful comments.
- 7.Cunliffe-Beamer TL: Biomethodology and surgical techniques. The Mouse in Biomedical Research. Edited by: Foster HL, Small JD, Fox JG. 1983, New York , Academic Press, 3: 402-Google Scholar
- 10.England GCW: Allen's Fertility and Obstetrics in the Dog. 1998, Oxford , Blackwell Science Ltd., 245-SecondGoogle Scholar
- 12.Davidson AP, Feldman EC: Ovarian and estrous cycle abnormalities in the bitch. Textbook of Veterinary Internal Medicine. Edited by: Ettinger SJ, Feldman EC. 1995, Philadelphia , W. B. Saunders, 2: 1607-1613. 4thGoogle Scholar
- 13.Purswell BJ: Differential diagnosis of canine abortion. Curr Vet Ther Small Anim Pract. 1992, 11: 925-929.Google Scholar
- 14.Johnston SD, Root Kustritz MV, Olson PNS: Canine and Feline Theriogenology. 2001, Philadelphia , W. B. Saunders CompanyGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.