Pregnancy following homologous prepubertal ovarian transplantation in the dog

Open Access
Short report

Abstract

In several canine models of hereditary human disease the homozygote dogs die prior to puberty, or have substantially reduced fertility. To create a clinically healthy animal that can be bred, but can also transmit the gene of interest, a model of homologous ovarian transplantation in prepubertal dogs was developed. Six dog leukocyte antigen (DLA) identical littermates underwent transplantation of ovarian cortical strips (n = 2) or the entire ovary (n = 4). Immunosuppression was maintained with cyclosporine and MMF in the immediate post-operative period and cyclosporine alone thereafter. All 6 dogs entered puberty and normal semiannual estrus cycles as demonstrated by both physical changes and increasing serum progesterone. Four dogs were bred to a proven stud male, and one became pregnant. Three viable fetuses with observable heartbeats were detected on ultrasound examination. Although the dog eventually aborted the litter, this work represents the first pregnancy achieved following a prepubertal ovarian transplant in the dog.

List of abbreviations

DLA

dog leukocyte antigen

LH

luteinizing hormone.

Notes

Acknowledgements

This work was supported by NIH 5R24HD047142 to Dr. Pullium. The authors thank Dr. Richard Fayrer-Hoskins for helpful comments.

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Copyright information

© Pullium et al; licensee BioMed Central Ltd. 2008

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Authors and Affiliations

  • Jennifer K Pullium
    • 1
    • 2
  • Ross Milner
    • 3
  • Gary A Tuma
    • 4
  1. 1.Department of Pathology and Laboratory MedicineEmory University School of MedicineAtlantaUSA
  2. 2.Center for Comparative Medicine and SurgeryMount Sinai School of MedicineNew YorkUSA
  3. 3.Department of Surgery, Division of Vascular SurgeryEmory University School of MedicineAtlantaUSA
  4. 4.Department of Surgery, Division of Plastic SurgeryEmory University School of MedicineAtlantaUSA

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