Prospective assessment of Y-chromosome microdeletions and reproductive outcomes among infertile couples of Japanese and African origin
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To compare the frequency of Y-chromosome microdeletions in Japanese and African azoospermic and oligozoospermic men and describe embryo characteristics and reproductive outcome following in vitro fertilization (IVF) with intracytoplasmic sperm injection (ICSI).
Our study was performed prospectively at two centers, a private IVF clinic and a university hospital. Japanese and African (Tanzanian) men with nonobstructive azoospermia (NOA) and oligozoospermia (concentration < 5 × 106 /ml) were evaluated for Y-chromosome microdeletions (n = 162). Of the 47 men with NOA, 26 were Japanese and 21 were Africans. Of the 115 men with oligozoospermia, 87 were Japanese and 28 were Africans. Reproductive outcomes of patients with Y-chromosome microdeletions were then compared with those of 19 IVF+ICSI cycles performed on couples with Y-chromosome intact males/tubal factor infertility which served as a control group.
Seven azoospermic and oligozoospermic patients had Y-chromosome deletions; the total number of deletions in the AZFc region was five. There was only one deletion in the AZFa region and one complete deletion involving all three regions (AZFa, b, and c) within AZF. In our study population, microdeletion frequency among Japanese men was 6.2% (95% CI, 4.25% – 14.45%), whereas no deletions were identified in the African group (95% CI, 0.0% – 7.27%). The difference between the two groups was not statistically significant, however. Embryos derived from ICSI utilizing sperm with Y-chromosome microdeletion showed reduced rates of fertilization, blastocyst development, implantation, and pregnancy compared to the Y-chromosome intact group, although these observed differences were not statistically significant.
The observed frequency of Y-chromosome microdeletion was 6.2% among Japanese azoospermic and oligozoospermic males; no microdeletions were identified among our African study patients. In this population of couples undergoing IVF+ICSI, there was no statistically significant difference in embryo characteristics or pregnancy outcome between patients with Y-chromosome microdeletion and those with an intact Y-chromosome.
We wish to thank Dr. Takafumi Utsunomiya, Yoko Kumasako and Keiko Hirotsuri of St. Luke hospital for their advice and technical help ; We also wish to thank Prof. Kensuke Yamamoto, Dr. Kazuo Aoki, Prof. Junichi Misumi and Akira Kono of Oita University school of Medicine for their help in study design and constant examination of our data; Drs. Ramzy E. Kisanga and Godfrey Lema of Muhimbili University of college science, Dar-es-salaam, Tanzania for their technical assistance while in Tanzania and San Francisco Edit http://www.sfedit.net for editing this manuscript.
- 2.Abma JC, Chandra A, Mosher WD, Peterson LS: Fertility, Family Planning and Women's Health: New data from the 1995 national Survey of Family Growth. Vital heath statistics. 1997, 23: 1-114.Google Scholar
- 8.Devroey P, Vandervorst M, Nagy P, Van Steirteghem AC: Do we treat the male or his gamete? In "Current Theory and Practice of ICSI". Hum Reprod. Edited by: Devroey P, Tarlatzis B, Van Steirteghem A. 1998, Oxford, UK, Oxford University Press, 13: 178-185. 10.1093/humrep/13.1.178.Google Scholar
- 20.Kobayashi K, Mizuno K, Hida A, Komaki R, Tomita K, Matsushita I, Namiki M, Iwamoto T, Tamura S, Minowada S: PCR analysis of the Y chromosome long arm in azoospermic patients: evidence for a second locus required for spermatogenesis published erratum appears in. Hum Mol Genet. 1995, 4 (5): 974-PubMedGoogle Scholar
- 26.Mulhall JP, Reijo R, Alagappan : Azoospermic men with deletion of the DAZ gene cluster are capable of completing spermatogenesis: fertilization, normal embryonic development and pregnancy occur when retrieved testicular spermatozoa are used for intracytoplasmic sperm injection. Hum Reprod. 1997, 12: 503-508. 10.1093/humrep/12.3.503.CrossRefPubMedGoogle Scholar
- 43.Bornman MS, Crewe-brown HH, Reif S, Mahomed MF, Broomker D, Schulenburg GW: Sexually transmitted diseases (STD) in infertile males attending the andrology clinic at Ga-Rankuwa Hospital. Archives of AIDS Research. 1993, 145-150.Google Scholar
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