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Retrovirology

, 8:O12 | Cite as

Interaction between HIV and its host: role for viral and cellular sncRNA

  • Monsef Benkirane
Open Access
Oral presentation
  • 1.1k Downloads

Keywords

Myeloid Cell Early Step Sensitive Cell Proteasomal Degradation Restriction Factor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

The primate lentivirus auxiliary protein Vpx counteracts an unknown restriction factor that renders human dendritic and myeloid cells largely refractory to HIV-1 infection. We recently identified Samhd1 as this restriction factor. Samhd1 is a protein involved in Aicardi-Goutière Syndrome (AGS), a genetic encephalopathy with symptoms mimicking congenital viral infection that has been proposed to act as a negative regulator of the ínterferon-stimulated DNA response. We show that Vpx induces proteasomal degradation of Samhd1. Silencing of Samhd1 in non-permissive cell lines alleviates HIV-1 restriction and is associated with a significant accumulation of viral DNA in infected cells. Concurrently, overexpression of Samhd1 in sensitive cells inhibits HIV-1 infection. The putative phosphohydrolase activity of Samhd1 is likely required for HIV-1 restriction. Vpx-mediated relief of restriction is abolished in Samhd1 negative cells. Finally, silencing of Samhd1 dramatically increases susceptibility of MDDCs to infection. Altogether, these results demonstrate that Samhd1 is an anti-retroviral protein expressed in cells of the myeloid lineage and inhibiting an early step of the viral life cycle.

Copyright information

© Benkirane; licensee BioMed Central Ltd. 2011

This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Authors and Affiliations

  • Monsef Benkirane
    • 1
  1. 1.Laboratoire de Virologie MoléculaireInstitut de Génétique HumaineMontpellierFrance

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