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Retrovirology

, 8:A118 | Cite as

Association of interleukin - 18 gene polymorphism with susceptibility or protective effect to HTLV-1 infection

  • Virgínia M D Wagatsuma
  • Daiani C Cilião-Alves
  • Maurício C Rocha–Junior
  • Rodrigo Haddad
  • Oswaldo M Takayanagui
  • Eduardo A Donadi
  • Dimas T Covas
  • Simone Kashima
Open Access
Meeting abstract

Keywords

Promoter Region Gene Polymorphism Genotypic Frequency Infected Patient Polymorphic Site 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Background

Polymorphisms in promoter region of IL-18 genes have been studied in many chronic inflammatory and infectious disorders. For instance, the IL-18 -137 GG and -607CC polymorphisms have been associated with elevated expression of IL-18, which could contribute for the inflammatory process and favor the antiviral effects of this cytokine. In this study, we evaluated the IL-18 promoter region -137C/G and -607A/C polymorphisms in HTLV-infected patients exhibiting (HAM) or not (HAC) symptomatic disease and in healthy controls.

Methods

The promoter region polymorphic sites (-137C/G and -607A/C) were evaluated by polymerase chain reaction - sequence specific primers (PCR-SSP) analysis using peripheral blood DNA obtained from HAC (54), HAM (44) and healthy control (150) individuals. Proviral load of infected patients (HAC and HAM) was determined by real-time PCR.

Results

The -607CC genotype was less frequent for HAC group (p=0.0501; OR=0.4890; CI=0.2480 to 0.9643), and infected patients (p=0.0232; OR=0.5207; CI=0.3033 to 0.8937) compared to healthy controls. The -607AC genotype was more frequent for HAC group (p=0.0387; OR=1.991; CI=1.043 to 3.801), and infected patients (p=0.0376; OR=1.757; CI=1.047 to 2.948) compared to healthy controls. No significant difference was observed for allelic and genotypic frequencies of the -137C/G among deferments groups. No significant difference was observed for allelic and genotypic frequencies of the -137C/G and -607A/C polymorphisms when correlated with proviral load.

Conclusion

The -607CC (high producer of IL-18) genotype exhibited protective effect against the infection, whereas the -607AC genotype conferred susceptibility to HTLV-1 infection.

Notes

Acknowledgements

Financial Support: FAPESP, CNPq, CTC/FUNDHERP and INCTC.

Copyright information

© Wagatsuma et al; licensee BioMed Central Ltd. 2011

This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Authors and Affiliations

  • Virgínia M D Wagatsuma
    • 1
  • Daiani C Cilião-Alves
    • 2
  • Maurício C Rocha–Junior
    • 1
    • 3
  • Rodrigo Haddad
    • 1
    • 2
  • Oswaldo M Takayanagui
    • 2
  • Eduardo A Donadi
    • 2
  • Dimas T Covas
    • 1
    • 2
  • Simone Kashima
    • 1
    • 3
  1. 1.Regional Blood Center of Ribeirão PretoRibeirão PretoBrazil
  2. 2.Faculty of Medicine of Ribeirão PretoUniversity of São PauloRibeirão PretoBrazil
  3. 3.Faculty of Pharmaceutical Sciences of Ribeirão PretoUniversity of São PauloRibeirão PretoBrazil

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