Retrovirology

, 2:P145 | Cite as

Circulating Human Vγ2/Vδ2 T Cells Express Cytoplasmic RANTES

  • Ilia Tikhonov
  • S Berg-Dixon
  • V Lukyanenko
  • C Deetz
  • D Pauza
Open Access
Poster presentation
  • 620 Downloads

Keywords

Effector Memory Central Memory Cytoplasmic Granule Memory Phenotype Memory Pool 

A likely process of chronic positive selection produces the highly biased peripheral blood γδ T-cell repertoire of adult human beings. The major blood subset expresses the Vγ2Vδ2 T-cell receptor and responds to phosphoantigen stimulation in the absence of MHC restriction. Chronic expansion of γδ T-cell pool is expected to produce a population of cells with the effector/memory phenotype. A CC chemokine RANTES is produced late after TCR stimulation of αβ T-cells, accumulates into the cytoplasm and represents a marker for non-naïve T-cells. We demonstrate here that the vast majority of peripheral human T-cells contain RANTES in the cytoplasmic granules. In vitro expansion after non-peptidic phosphoantigen stimulation mimics the normal γδ T cell response to pathogens, and produces polyclonal Vγ2/Vδ2 cell population uniformly positive for cytoplasmic RANTES. These cells readily release RANTES from cytoplasmic depots into the culture medium after TCR stimulation. The presence of stored RANTES suggests a memory phenotype and may mediate effector functions of circulating Vγ2/Vδ2 cells. Phosphoantigen-responsive Vγ2/Vδ2 T cells represent 1 in 40 of circulating CD3+ lymphocytes; this is the dominant central memory population in primate peripheral blood that can evolve directly into an effector memory pool.

Copyright information

© The Author(s) 2005

Authors and Affiliations

  • Ilia Tikhonov
    • 1
  • S Berg-Dixon
    • 1
  • V Lukyanenko
    • 2
  • C Deetz
    • 1
  • D Pauza
    • 2
  1. 1.University of Maryland Biotechnology InstituteInstitute of Human VirologyUSA
  2. 2.Medical Biotechnology CenterUniversity of Maryland Biotechnology InstituteUSA

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