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Retrovirology

, 12:P63 | Cite as

Are seroindeterminate western blot patterns in human T-Cell lymphotropic virus type 1 (Htlv-1) infected individuals associated with low proviral load levels?

  • Camila Cánepa
  • Jimena Salido
  • Sindy Fraile
  • Matías Ruggieri
  • Mirna Biglione
  • Carolina Berini
Poster presentation
  • 291 Downloads

Keywords

Asymptomatic Carrier Proviral Load Weak Immune Response Reactive Screening Blot Pattern 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

For HTLV-1/2 diagnosis, reactive screening results are generally confirmed by Western Blot (WB). However, the large number of indeterminate WB patterns is still a problem worldwide. The aim of this study was to determine whether low levels of proviral load (PVL) are associated with seroindeterminate results by WB. PVL was determined in 49 HTLV-1 samples confirmed by n-PCR and classified as G1: positive by WB from individuals with disease (n= 27, 5 ATLL and 22 HAM/TSP); G2: positive by WB from asymptomatic carriers (n= 18); and G3: seroindeterminate samples by WB from asymptomatic carriers (n= 4). The viral gen pol and albumin were quantified by real-time SYBR Green PCR (ABI Prism System-AppliedBiosystems 75). Calibration curves were constructed using a DNA stock from MT2 cell line (Limit of quantification: 3 pol copies/reaction-R2> 0.99) and the analysis was performed by Krustall Wallis (GraphPad Prism v.5). Median PVL values were 4.03, 1.58 and 0.15 copies of HTLV-1/1 PBMCs fo r G1, G2 and G3, respectively. Correlation between PVL and age of patients (S= 0.61) was found for G1 samples. PVL median values were significantly different between the three groups (p= 0.3); the difference was also observed (p = 0.5) when considering HTLV-1 positive samples by WB [G1 + G2] as a single group. Even though a seroconversion could not be discarded in seroindeterminate cases, a low viral replication rate due to other factors could trigger a weak immune response, thus causing seroindeterminate WB patterns. The present study clearly demonstrates that such cases could be associated to a low HTLV-1 PVL and that the molecular analysis is a necessary tool to reach a final diagnosis.

Copyright information

© Cánepa et al. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Authors and Affiliations

  • Camila Cánepa
    • 1
  • Jimena Salido
    • 1
  • Sindy Fraile
    • 1
  • Matías Ruggieri
    • 1
  • Mirna Biglione
    • 1
  • Carolina Berini
    • 1
  1. 1.Instituto de Investigaciones Biomédicas en Retrovirus y SIDA (INBIRS), UBA-CONICETBuenos AiresArgentina

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