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Retrovirology

, 11:P99 | Cite as

Comparative infection of autologous primary T cells and monocytes derived DCs using cell-free virus preparation, viral biofilm, pseudotyped-virus or viral synapse

  • Sandrine Alais
  • Hélène Dutartre
  • Renaud Mahieux
Open Access
Poster presentation
  • 471 Downloads

Keywords

Dendritic Cell Cell Infection Early Step Viral Preparation Viral Source 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

HTLV-1 infected T cells and blood dentritic cells (DCs) can be found in HTLV-1 carriers. It is currently believed that T cell infection requires cell-cell contact, while DCs could also be infected with cell-free virus. However, a comparative study using different modes of viral preparation for infecting human primary autologous T and DC cells has not been performed. Supernatant from HTLV-1 chronically infected T cells, cell conjugates between HTLV-1 infected cells and target cells, or purified viral biofilm were used side-by-side to infect autologous human primary lymphocytes and monocytes-derived dendritic cells. To monitor the early steps of viral infection, we also used pseudotyped viruses carrying either the HTLV-1 or the VSV envelopes. Infection was then followed by flow cytometry, ELISA, immunofluorescence, real-time PCR and alu-PCR. Our first data show that, depending on the source of viruses, lymphocytes have distinct susceptibility to HTLV-1 infection. The impact of the viral source in the spread of HTLV-1 infection will be discussed.

Copyright information

© Alais et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Authors and Affiliations

  • Sandrine Alais
    • 1
  • Hélène Dutartre
    • 1
  • Renaud Mahieux
    • 1
  1. 1.Oncogenèse Rétrovirale, label « Ligue Nationale Contre le Cancer », CIRI, Labex Ecofect, INSERM U1111-CNRS UMR5308Université Lyon-1, Ecole Normale SupérieureLyon, Cedex 07France

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