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Retrovirology

, 11:P139 | Cite as

CD25+CCR4+ cells as a marker of HTLV-1-infected cells in peripheral blood

  • Huseini Kagdi
  • Anat Melamed
  • Silva Hilburn
  • Maria A Demontis
  • Charles Bangham
  • Graham Taylor
Open Access
Poster presentation

Keywords

Cell Count Viral Infection Infected Cell CD25 Expression Virus Type 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

The diagnosis of Human T cell Lymphotropic Virus type 1 (HTLV-1) infection is based on serology and PCR. The burden of HTLV-1 viral infection is monitored by measuring the proviral load [PVL] in peripheral blood. This assay is not useful for isolation of HTLV-1-infected cells. HTLV-1 Tax upregulates CD25 expression on infected CD4+ T cells. However, CD4+CD25+ is also the phenotype of activated T cells. CCR4 is a chemokine receptor expressed on subsets of activated T cells. HTLV-1-infected cells secrete the CCR4 ligand CCL22, and preferentially infect CCR4+ Cells. We studied the expression of CD25 and CCR4 in lymphocytes and its relation to PVL. We performed 11-colour immunophenotyping and HTLV-1 PVL quantification on 53 samples obtained from 36 HTLV-1 infected patients, [10 asymptomatic carriers (AC); 11 patients with HTLV-1-associated myelopathy (HAM); 4 co-infected with HIV; 11 with chronic/smouldering adult T-cell leukaemia/lymphoma (ATL)] and 3 uninfected individuals. Increased frequency of CD25+CCR4+ T cells [median: 14.7%, range: 1.95-91.3%] was observed in all HTLV-1 infected patients; the frequency correlated with PVL [Spearman r=0.89, P <0.001, Linear R2 = 0.61]. CD4+ and CD8+ cells from 12 patients [6ACs and 6 HAMs] were separated for PVL estimation. CD25+ CCR4+ cell counts correlated closely with PVL in CD4+ cells [Spearman r=0.816, P <0.001, linear R2=0.886] but not in CD8+ cells. Frequency of CD25+CCR4+ T cells correlated with PVL change in treated ATL patients. We conclude that CD25+CCR4+ cells can be used as a specific marker for monitoring of HTLV-1 infection and isolation of HTLV-1 infected cells.

Copyright information

© Kagdi et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Authors and Affiliations

  • Huseini Kagdi
    • 1
  • Anat Melamed
    • 1
  • Silva Hilburn
    • 1
  • Maria A Demontis
    • 1
  • Charles Bangham
    • 1
  • Graham Taylor
    • 1
  1. 1.Infectious Diseases and Immunology, Faculty of MedicineImperial CollegeLondonUK

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