Measurement of the intracellular lifetime of water to estimate myocardial cell size is not feasible in humans using clinical contrast agent doses at 1.5T
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KeywordsCardiovascular Magnetic Resonance Blood Pool Absolute Residual Extracellular Volume Fraction Extracellular Contrast Agent
Measurement of the myocardial extracellular volume fraction (ECV) assumes a dynamic equilibrium with rapid exchange of an extracellular contrast agent between myocardium and blood. This equilibrium would be manifested by a linear relationship between 1/T1 (R1) of myocardium and 1/T1* (R1*) of blood. This relationship has been shown to be non-linear for high R1 values in mice, and this has been used to calculate the intracellular lifetime of water and estimate myocardial cell size. We sought to determine whether the relationship between R1 of myocardium and R1* of blood is linear or non-linear in patients scanned under clinical conditions.
Consecutive patients referred for clinical cardiovascular magnetic resonance (CMR) evaluation of suspected heart disease were prospectively enrolled. CMR was undertaken at 1.5T (Siemens Aera) using a modified Look-Locker inversion recovery (MOLLI) sequence before and approximately 3, 8 and 16 minutes after an intravenous bolus of a gadolinium-based extracellular contrast agent (Dotarem, gadoteric acid, 0.2 mmol/kg). Patients were included if the CMR findings were normal and there were no severe artefacts. Patients underwent T1- and T1*-mapping with MOLLI. A region of interest was placed in the left ventricular blood pool (T1*) and midmurally in the myocardium (T1) of a midventricular short-axis slice at all time points.
In the study population (n=16, age 52 ± 18 years, 63% male), there was a linear relationship between R1 of myocardium and R1* of blood (R1 myocardium = 0.441 x R1* blood + 0.726, R2=0.979, p<0.001, see figure). Non-linear regression (R1 myocardium = 0.338 x R1* blood^1.13 + 0.835, R2=0.981, p<0.001) did not differ from linear regression (p=0.93 for t-test of absolute residuals). Similar results were obtained when using R1 instead of R1* of blood. Mean ± SD T1 of the myocardium was 981 ± 27 ms pre-contrast, 307 ± 32 ms 3 minutes post-contrast, 419 ± 27 ms 8 minutes post-contrast, and 475 ± 32 ms 16 minutes post-contrast.
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