Magnetic resonance and bioluminescence imaging of macrophage homing to experimental abdominal aortic aneurysms
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KeywordsAbdominal Aortic Aneurysm Abdominal Aortic Aneurysm Prussian Blue Aortic Diameter Iron Oxide Particle
Macrophage infiltration is a prominent feature of abdominal aortic aneurysm (AAA) progression. We used a multimodality imaging approach - magnetic resonance (MRI) and bioluminescence (BLI) - to study macrophage homing and accumulation in experimental AAA disease.
Employ cellular imaging methods to track and image macrophages in AAA disease.
Murine AAAs were created via intra-aortic infusion of porcine pancreatic elastase and aortic size was monitored by ultrasound on post-op day (POD) 3, 7, and 14. Mice were imaged after injection of prepared peritoneal macrophages. For BLI, macrophages were from transgenic mice expressing luciferase, with injection on POD 1 and imaging at POD 3, 7, and 14 by in situ BLI (AAA N=17, sham control N=15). For MRI, macrophages were labeled with iron oxide particles (Feridex), with injection on POD 13 and imaging at POD 14 by in vivo 7 T MRI (AAA N=8, sham N=5). Mice were sacrificed after imaging for histological analysis.
MRI detects homing of macrophages to AAA in vivo, while in situ BLI can follow macrophage accumulation over time. Thus, MRI and BLI provide complementary approaches to track macrophage biology in experimental AAAs.
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