Expression of IL-1 family members upon stimulation with IL-17 differs in keratinocytes derived from psoriasis patients and healthy donors

  • P Muhr
  • J Zeitvogel
  • T Werfel
  • M Wittmann
Open Access
Poster presentation

Keywords

Psoriasis Healthy Individual Inflammatory Mediator Healthy Donor Hair Follicle 

Background

A number of studies have challenged the T cell centred pathogenetic view of psoriasis by the finding that epithelium-expressed genes are intimately involved in the inflammatory process. IL-17 is an important inflammatory mediator in skin psoriasis.

Objective

IL-17 is known to act on keratinocytes and we were interested in its impact on expression of pro- and anti-inflammatory IL-1 family members.

Methods

We compared human primary keratinocytes derived from psoriasis patients and healthy individuals using qRT-PCR and ELISA.

Results

In the presence of IL-17 psoriasis derived keratinocytes showed a significantly higher induction of the pro-inflammatory members IL-1F6 and IL-1F9 compared with those from healthy individuals but not of anti-inflammatory members IL-1F5, IL-1F7 or IL-1F3. Both basal, as well as IL-17 induced production of IL-1F2/IL-1β and IL-1F1/IL-1α were found to be significantly lower in psoriasis keratinocytes.

Conclusion

As keratinocytes were derived from epidermal stem cells of the hair follicles and obtained from non-lesional sites, differences found are likely to present an intrinsic feature of psoriasis epithelium. Our data suggest that the significance of IL-1 members as therapeutic targets in psoriasis conditions merits further and thorough investigation.

Copyright information

© Wittmann et al; licensee BioMed Central Ltd. 2010

This article is published under license to BioMed Central Ltd.

Authors and Affiliations

  • P Muhr
    • 1
  • J Zeitvogel
    • 1
  • T Werfel
    • 1
  • M Wittmann
    • 1
    • 2
  1. 1.Dept. of Dermatology and Allergy, Division of Immunodermatology and Allergy ResearchHannover Medical SchoolHannoverGermany
  2. 2.Institute of Molecular and Cellular BiologyFaculty of Biological Sciences, University of LeedsLeedsUK

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