Microbial Cell Factories

, 5:P89 | Cite as

Expression of functional recombinant rabies virus glycoprotein in Drosophila melanogaster S2 cells

  • Adriana Y Yokomizo
  • Soraia AC Jorge
  • Renato M Astray
  • Mariza AG Santos
  • Irene Fernandes
  • Orlando G Ribeiro
  • Denise SPQ Horton
  • Aldo Tonso
  • Carlos A Pereira
Open Access
Poster Presentation
  • 1.1k Downloads

Keywords

Hygromycin Rabies Rabies Virus Actin Promoter Hygromycin Selection 

Background

The rabies virus belongs to the genus Lyssavirus from the Rhabdoviridae family and is widely distributed in nature infecting mammals. Upon infection it can be transmitted to animals or humans and leads to a fatal disease that nowadays has no treatment. Vaccines are commercially available and prevent the disease in animals and humans. Protocols for human or veterinarian vaccine manufacturing evolved from animal tissue homogenates to cell culture technology and today recombinant viral proteins and DNA vaccines are under investigation. The evidence that rabies virus infects and can cause disease in animals and humans, being neutralized by an immune response mounted by very similar vaccines opens a great possibility of testing new vaccines first in experimental animals prior to use in humans [1].

Results

Recombinant rabies vírus glycoprotein (rGPV) was expressed in Drosophila melanogaster Schneider 2 (S2) cells. The cDNA encoding the GPV gene was cloned in expression plasmids under the control of the inducible metallothionein promoter (Mt) or the constitutive actin promoter (Ac). These were alternatively co-transfected into S2 cells together with a hygromycin selection plasmid. Selected S2 cell populations (S2MtGPV or S2AcGPV) had a decreased ability to grow and consume substrates, when compared to the non transfected cells (S2). They were shown, by PCR, to express the GPV gene and mRNA as well as, by immunoblotting, to synthesize the rGPV in its expected molecular weight of 65 kDa. ELISA kinetic studies showed the rGPV expression in cell lysates and supernatants attaining concentrations ranging from 150 to 300 μg of rGPV/L. By flow cytometry analysis, about 30% of the cells in these populations were shown to express the rGPV in their membrane. Cell populations selected by limiting dilution expressed higher rGPV yields. Mice immunized with S2MtGPV or S2AcGPV derived rGPV were capable of mounting a protective immune response characterized by the synthesis of antibodies reacting against the rabies virus. Immunization led to protection against rabies virus experimental infection in challenge studies.

Conclusion

The data presented here show that S2 cells are suitable hosts for the rGPV expression allowing its synthesis in a high degree of physical and biological integrity.

Notes

Acknowledgements

This work was supported in part by grants and scholarships from the FAPESP, CNPq, CAPES and Fundação Butantan. C. A. Pereira is recipient of CNPq research fellowships. We thank M.J.S. Leme for technical assistance performing "in vivo" challenge assays.

References

  1. 1.
    Warrel MJ, Warrel DA: Rabies and other lyssavirus diseases. Lancet. 2004, 363: 959-969. 10.1016/S0140-6736(04)15792-9.CrossRefGoogle Scholar

Copyright information

© Yokomizo et al; licensee BioMed Central Ltd. 2006

This article is published under license to BioMed Central Ltd.

Authors and Affiliations

  • Adriana Y Yokomizo
    • 1
  • Soraia AC Jorge
    • 1
  • Renato M Astray
    • 1
  • Mariza AG Santos
    • 1
  • Irene Fernandes
    • 2
  • Orlando G Ribeiro
    • 3
  • Denise SPQ Horton
    • 4
  • Aldo Tonso
    • 5
  • Carlos A Pereira
    • 1
  1. 1.Laboratório de Imunologia Viral, Instituto ButantanSão PauloBrasil
  2. 2.Laboratório de Imunopatologia, Instituto ButantanSão PauloBrasil
  3. 3.Laboratório de Imunogenética, Instituto Butantan,São PauloBrasil
  4. 4.Serviço de Controle de Qualidade, Instituto ButantanSão PauloBrasil
  5. 5.Departamento de Engenharia QuímicaEscola Politécnica, Universidade de São PauloBrasil

Personalised recommendations