Advertisement

Microbial Cell Factories

, 5:P32 | Cite as

Optimisation of substrate feeding in shake flask cultures of Pichia pastoris for recombinant protein production

  • Monika Bollok
  • Maria Ruottinen
  • Mirja Krause
  • Antti Vasala
  • Eija-Riitta Hämäläinen
  • Antje Neubauer
  • Johanna Myllyharju
  • Peter Neubauer
Open Access
Poster Presentation
  • 3k Downloads

Keywords

Shake Flask Pichia Pastoris Prolyl Shake Flask Culture Feeding Protocol 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Background

Pichia pastoris is used as a common host for production of recombinant proteins. Gene expression is mostly controlled by the AOX promoter through methanol addition. The aim of this study was to investigate whether the generally applied methanol addition protocol is optimum and whether the expression in shake flasks can be improved by applying a different feeding scheme.

Therefore we applied the recently described wireless on-line monitoring wireless system (SENBIT® which allows the application of standard sensors such as pH and pO2 in shake flasks [1]. Moreover, a sensitive sandwich hybridization technology was used for the quantitative analysis of the expression level for process relevant marker genes which to provide data about the physiological state of the cultures and hereby a better understanding of the microbial responses.

Results

The impact of the feeding protocol was studied in shake flask cultures of Pichia pastoris for recombinant collagen production with methanol as inducer and carbon source. On-line measurement of pO2 revealed that the standard methanol feeding protocol is not favourable. The culture is starved for long times for methanol and also oxygen may be depleted shortly after a methanol pulse. A fed-batch like feeding procedure was developed by applying a computer controlled micropump system for semi-continuous addition of methanol. As a result the amount of collagen was improved. Furthermore, also the expression of collagen prolyl 4-hydroxylase, a collagen modifying enzyme was strongly increased which resulted in collagen of higher stability. The improvement of the culture conditions with the new feeding protocol were verified by semi-quantitative analysis of different cellular mRNAs.

Conclusion

Regular feeding of small amounts of methanol in a semi-continuous way improves the behaviour of recombinant cultures of Pichia pastoris and increased the amount and quality of collagen in our study. We propose that this method is generally favourable for the optimisation of gene expression in Pichia pastoris shake flask cultures.

Notes

Acknowledgements

This study was supported by the TEKES "Neobio" programme and a grant to MB by the Academy of Finland.

References

  1. 1.
    Vasala A, Panula J, Bollok M, Illman L, Hälsig C, Neubauer P: A new wireless system for decentralised measurement of physiological parameters from shake flasks. Microb Cell Fact. 2006, 5: 8- 10.1186/1475-2859-5-8.CrossRefGoogle Scholar

Copyright information

© Bollok et al; licensee BioMed Central Ltd. 2006

This article is published under license to BioMed Central Ltd.

Authors and Affiliations

  • Monika Bollok
    • 1
  • Maria Ruottinen
    • 1
  • Mirja Krause
    • 1
  • Antti Vasala
    • 1
  • Eija-Riitta Hämäläinen
    • 1
  • Antje Neubauer
    • 2
  • Johanna Myllyharju
    • 2
  • Peter Neubauer
    • 1
  1. 1.Dept. Process & Environm. EnginBioprocess Engineering LaboratoryUSA
  2. 2.University of OuluOuluFinland

Personalised recommendations