Heating as a rapid purification method for recovering correctly-folded thermotolerant VH and VHH domains
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Recombinant antibodies from Camelidae (VHHs) are potentially useful tools for both basic research and biotechnological applications because of their small size, robustness, easy handling and possibility to refold after chemio-physical denaturation. Their heat tolerance is a particularly interesting feature because it has been recently related to both high yields during recombinant expression and selective purification of folded protein.
Purification of recombinant RE3 VHH by heat treatment yielded the same amount of antibody as purification by affinity chromatography and negligible differences were found in stability, secondary structure and functionality. Similar results were obtained using another class of thermotolerant proteins, the single domain VH scaffold, described by Jespers et al. . However, thermosensitive VHs could not withstand the heat treatment and co-precipitated with the bacterial proteins. In both cases, the thermotolerant proteins unfolded during the treatment but promptly refolded when moved back to a compatible temperature.
Heat treatment can simplify the purification protocol of thermotolerant proteins as well as remove any soluble aggregate. Since the re-folding capability after heat-induced denaturation was previously correlated to higher performance during recombinant expression, a unique heating step can be envisaged to screen constructs that can provide high yields of correctly-folded proteins.
KeywordsPhage Display Recombinant Expression Recombinant Antibody Immobilize Metal Affinity Chromatography Immune Library
There has been an increased interest in antibodies in recent years, both because of their clinical applications and their use in basic research . Conventional antibodies (mono- and poly-clonal) present several shortcomings, such as their bulky structure, tedious and expensive preparation, limited opportunities to introduce mutations, and the immunogenic response they can induce when used in therapy. For these reasons, a techniques aimed at recombinant expression of antibodies selected from both immunized and naïve/synthetic libraries can be a convenient alternative .
The most common format for antibody recombinant expression is probably the single chain antibody (scFv), in which the heavy and light variable regions are linked together. Polybodies, with higher avidity for the antigen than single scFv molecules, can be obtained by varying the length of the linker or by connecting via a flexible hinge to an amphipathic helix [3, 4]. ScFvs have been widely used to identify antigens in in vitro and in vivo experiments and to deliver active molecules against tumor markers in model animals .
The variable heavy (VHH) format is an alternative that exploits the particularity of the Camelidae immunogenic system. The animals belonging to this family possess, beside antibodies of conventional structure, also antibodies formed only by the heavy chain . In this case all of the information for the specific recognition of the antigen is present in the heavy chain variable region. In contrast to the variable heavy chain of conventional antibodies (VH), which pairs with the light chain, the VHHs have evolved in the absence of such a counterpart. This results in a higher intrinsic stability of VHHs, in comparison to scFvs, when the recombinant antibodies are expressed in bacteria . Since the paratope is mostly restricted to the extruding CDR3 region, VHHs preferentially bind antigens in small cavities otherwise not accessible to conventional and scFv antibodies. For example, the active sites of enzymes . Furthermore, the ease in cloning and preparation of fusion constructs makes VHHs promising molecules for biotechnological applications like antibody-based microarrays and biosensors .
We wished to obtain stable recombinant antibodies for in vitro and in vivo studies. However, several of the recombinant antibodies expressed in bacteria are structurally unstable, signifying that good binders selected by phage display cannot be successfully used for practical applications. Therefore, VHHs were first selected by phage display from an immune library (Olichon and Surrey, unpublished data), and then an innovative approach based on thermotolerance was used to investigate their stability after recombinant expression.
Recently Jespers et al.  showed that thermotolerance of VH domains correlated with their yields of recombinant soluble protein. We were able to purify recombinant proteins fused to Archaea partners by heating E. coli lysates and to show that the treatment enabled, at the same time, to select monodispersed proteins because aggregated fusion proteins precipitated during the heat treatment . VHHs are thermotolerant proteins and the results reported in this paper show that a heating step can be used to purify them and the VH domains, preserving their structure and monodispersity.
Subcloning, expression, and purification of VHH and VH constructs
The VH constructs C36, C47, and DP47a were a kind gift of Dr. Winter. The VHH RE3 was isolated by a phage display screen of a VHH immune library cloned into the pHEN4 phagemid (; Olichon, unpublished data). The sequence corresponding to RE3 was subcloned into the pHEN6 vector and this transformed into XL Blue competent cells. Transformed cells were used to inoculate Terrific Brot. The culture was induced with 1 mM IPTG when the OD600 reached 0.5 and grown overnight at 28°C. The pellet corresponding to a 1 L culture was recovered by centrifugation and initially resuspended in 5 mL of TES buffer (0.2 M TrisHCl, pH 8.0, 0.5 mM EDTA, 0.5 M sucrose) before freezing in liquid nitrogen. It was then thawed on ice and 5 mL TES plus 5× EDTA-free protease inhibitor cocktail (Roche) were added. After 30 min incubation on ice, osmotic shock was induced by supplementing the cell suspension with 15 mL of 1:4 diluted TES buffer. After further 30 min incubation on ice, the cells were centrifuged 20 min at 30,000 g and the supernatant was recovered. NaCl and imidazole were added to a final concentration of 250 mM and 10 mM, respectively, and the fraction was purified by immobilized metal affinity chromatography (IMAC) using a HiTrap Chelating column (Amersham) and FPLC. The eluted fraction was desalted in 50 mM TrisHCl, pH 8.0, 50 mM NaCl and 10% glycerol and checked by SDS-PAGE. Its concentration was estimated after measurement of the absorbance at 280 nm.
The gel filtration experiments were performed using a Superpose 12 column (GE Healthcare) coupled to an FPLC system.
Heat purification of both RE3 and VHs was performed by heating the supernatants recovered after centrifugation of the bacterial periplasmic fraction and adding of PEG 6000 (10% final volume). Samples were heated 15 min at 70°C and thereafter cooled on ice for 20 min before pelleting the denatured proteins . Thermotolerant proteins were recovered from the supernatant and analyzed by SDS-PAGE.
An ELISA test was performed according to  using both heat-treated and untreated RE3. A VHH directed against lysozyme was used as a control. VHHs were detected using an anti-His monoclonal antibody (Qiagen) and a secondary anti-mouse HRP conjugated antibody (Amersham).
Fluorimetric and CD analyses
The fluorimetric assay proposed by Nominé et al.  has proved to be a reliable and simpler method than size exclusion chromatography for estimating the aggregation degree of proteins in solution . It is the ratio between the adsorbance at 280 nm (light scattering due to soluble aggregates) and that at 340 nm (specific absorbance of the accessible aromatic groups). The measurements were performed using an AB2 Luminescence Spectrometer (Aminco Bowman). Samples were excited at 280 nm and the emission spectra between 260 and 400 nm were recorded.
Standard far-UV CD spectra of the recombinant antibodies were recorded at 20°C using a Jasco J-710 spectrophotometer and cuvettes of 1 mm pathlength. 15 runs were accumulated for each sample and three independent repeats were performed to confirm the results. The spectra of the unfolded proteins were determined after having increased the temperature to 95°C using a Peltier heater and those of the refolded proteins after a further incubation of 30 min at 20°C. Capped cuvettes were used to prevent sample evaporation.
The wavelength for which the difference of ellipticity value between the folded and unfolded states was maximum was selected for progressively heating the samples and to identify the melting temperature (Tm) of the protein. The temperature was increased at a rate of 30°C/hour from 15 to 95°C and the ellipticity values were recorded at every increase of 0.2°C.
Purification of the RE3 VHH binder
The RE3 binder was first selected after panning a llama VHH phage display library using Ran-GTP, a protein involved in mitotic spindle assembly. It was sub-cloned in pHEN6 for expression in bacterial periplasm and showed both high affinity and specificity in enzyme-linked immunosorbant assay (ELISA) (Olichon, unpublished).
These results confirm that the antibody was expressed in a stable and native form in the bacterial periplasm and that its native structure was preserved during affinity purification and recovered after denaturation. However, its stability and monodispersity were strictly dependent on salt concentrations.
VH antibodies can be purified by heat treatment
Binders belonging to a specific class of VH antibodies  have been shown to possess a large variability in their capability to refold after heat denaturation. In particular, the authors noticed a strong correlation between heat tolerance and yields of soluble antibodies expressed recombinantly in bacteria. We thought that it would be interesting to compare the heat tolerance features of RE3 with those of the described VHs.
Discussion and conclusion
The data show that both C47 and RE3 can withstand heat treatment. In particular, they are denatured but can correctly refold after heat denaturation and this feature enables their differential purification because the heating also denatures and precipitates thermosensitive bacterial proteins. The stability of RE3 seems to be dependent on the conditions of the buffer, and NaCl is necessary to prevent its progressive polymerization. Therefore, the robustness of RE3 under optimal conditions does not implicate that VHH is easy to handle and negative results obtained in some in vitro binding experiments might derive from its instability under the experimental conditions. Recently, a stable framework for the expression of VHH intrabodies has been proposed and a grafting strategy was suggested to transfer the CDR regions from any interesting but instable binder of the same HcAb subfamilly into such a structure . It would be useful to challenge this framework for its buffer-tolerance as well, because the experience with RE3 clearly indicates that solubility and stability measured under optimal conditions are misleading whenever the application conditions significantly differ.
The original paper by G. Winter and co-workers indicates that only heat-denatured VHs that could correctly refold had structural features compatible with productive recombinant expression in bacteria . Now we complete this analysis by showing that not only the selection but even the purification can be performed using a single heating step. Therefore, the reported experiments enable an interesting strategy for simplifying protein purification and combining it with quality control . Such a strategy is based on the observation that not only the binding efficiency of RE3 was not inhibited by a heat treatment (15 min at 75°C) sufficient to denature the bacterial proteins, but other llama VHHs remained active after a heat treatment at 90°C  or were able to bind their substrate at 70°C . Similarly, the binding capacity of VHs is not altered by a 10 min treatment at 80°C . Furthermore, the results of the present work confirm those of the recent report showing that only monodispersed thermotolerant proteins can withstand a heat treatment . It also confirms that the heat-purified antibodies had the same secondary structure and low aggregation value typical of their respective affinity purified antibodies. Therefore, we have demonstrated that heat treatment can be applied for the simultaneous purification and selection of native folded and active proteins belonging to thermotolerant classes such as VHs and VHHs (our study), as well as fusions with Archaea proteins [9, 11].
Heat purification is not only a fast and inexpensive alternative to conventional chromatography but, when applicable, can also simplify the cloning strategy. Since no tag is needed for affinity purification, it is not necessary to produce tagged proteins that must be buffer exchanged, digested and re-purified before, for instance, being used in crystallography. The efficiency of heat purification with regard to the possibility to eliminate the endotoxins from bacterial recombinant protein preparations will be the object of specifically dedicated experiments.
It seems that thermotolerance, defined as the capability to refold after heat-denaturation, is sufficient for selective heat-dependent protein recovery. However, whilst thermoresistance is a very peculiar feature of extremophiles, thermotolerance can be identified even in proteins expressed in thermosensitive organisms, as with VHs and VHHs. We expect that other classes of thermotolerant proteins will be identified or their thermotolerance specifically improved by the addition of suitable compatible osmolytes .
The authors wish to thank Dr. G. Winter for having provided the VH antibodies and Kendra Swirsding for her assistance with the manuscript.
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