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BMC Infectious Diseases

, 12:P9 | Cite as

Development of a vaccine delivery system using hepatitis B core antigen based VLPs to deliver mycobacterial antigens

  • Dhananjayan Dhanasooraj
  • R Ajay Kumar
  • Sathish Mundayoor
Open Access
Poster presentation

Keywords

Tuberculosis Mycobacterium Tuberculosis Culture Filtrate Sera Fusion Construct 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Background

Growing prevalence of TB and the emergence of XDR-TB have stimulated substantial efforts to develop better vaccines for TB. Recent researches have shown that some of the antigenic proteins and fusion of different proteins produced by Mycobacterium tuberculosis can give protection in animal models when administrated with specific adjuvants. In the present study, we explored the use HBcag-VLPs for delivery of tuberculosis antigens.

Methods

HBcVLPs bearing ESAT-6 and CFP-10 were constructed using PCR and recombinant DNA methods. Proteins were expressed in E. coli and purified. VLPs formation was confirmed with TEM. BALB/c mice were immunized with VLPs and controls without any adjuvants. Sera were analysed for antibody responses (ELISA). Splenocytes were cultured and restimulated with purified antigens and CF (culture filtrate) of M.tb. The cell proliferation was measured using cell proliferation assay kit and the culture supernatants were analysed for IL-2, IFN-γ and TNF.

Results

The recombinant VLP induces preferentially a Th1-type immune response against mycobacterial antigen even though Th2 has been reported as the predominant response in BALB/c mice. IFN-γ, IL-2, TNF and proliferation were significantly higher in mice immunised with HBcVLPs-M. tuberculosis antigen. Restimulation with mycobacterial CF also produced the same effect.

Conclusion

The humoral and cellular responses suggest that the VLP containing fusion constructs generated immune response in a Th1 dependent manner. By virtue of its self-adjuvant nature, HBc VLPs are a better vaccine delivery system for use with newer antigens identified in the course of recent developments in subunit protein vaccine research in tuberculosis.

Copyright information

© Dhanasooraj et al; licensee BioMed Central Ltd. 2012

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Authors and Affiliations

  • Dhananjayan Dhanasooraj
    • 1
  • R Ajay Kumar
    • 1
  • Sathish Mundayoor
    • 1
  1. 1.Mycobacterium Research GroupRajiv Gandhi Centre for BiotechnologyThiruvananthapuramIndia

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