Nanoparticle penetration and transport in living pumpkin plants: in situsubcellular identification
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In recent years, the application of nanotechnology in several fields of bioscience and biomedicine has been studied. The use of nanoparticles for the targeted delivery of substances has been given special attention and is of particular interest in the treatment of plant diseases. In this work both the penetration and the movement of iron-carbon nanoparticles in plant cells have been analyzed in living plants of Cucurbita pepo.
The nanoparticles were applied in planta using two different application methods, injection and spraying, and magnets were used to retain the particles in movement in specific areas of the plant. The main experimental approach, using correlative light and electron microscopy provided evidence of intracellular localization of nanoparticles and their displacement from the application point. Long range movement of the particles through the plant body was also detected, particles having been found near the magnets used to immobilize and concentrate them. Furthermore, cell response to the nanoparticle presence was detected.
Nanoparticles were capable of penetrating living plant tissues and migrating to different regions of the plant, although movements over short distances seemed to be favoured. These findings show that the use of carbon coated magnetic particles for directed delivery of substances into plant cells is a feasible application.
KeywordsCorrelative Image Application Point Mesoporous Silica Nanoparticles Nanoparticle Aggregate Range Transport
Nanobiotechnology was born as a hybrid discipline, a combination of biotechnology and nanoscience. In recent years, nanoparticles with sizes typically below 100 nm, have been applied in several fields of bioscience and biomedicine, with an increasing number of commercial applications . Advances have been made in the field of biomedicine, including the development of tools for pathogen bio-detection, tissue engineering and MRI contrast enhancement . Special interest have been focused on those applications developed for targeted delivery of substances and drugs, implying direct movement of nanoparticles to specific organs [3, 4, 5].
The possibility of targeting the movement of nanoparticles to specific sites of an organism paves the way for the use of nanobiotechnology in the treatment of plant diseases that affect specific parts of a plant. Different procedures have made use of nanoparticles in plants, such as the controlled release of bioactive substances in solid wood [6, 7, 8] and plant transformation through bombardment with gold or tungsten particles coated with plasmidic DNA . In recent years, a breakthrough has been made as a result of Torney et al.  who were able to control the intracellular release of substances into protoplasts using mesoporous silica nanoparticles. Despite these advances, the delivery of nanoparticles into plant tissues has been limited to methods involving bombardment [9, 10], a methodology that does not allow massive application of particles in large numbers of plants, thus being of little use for agronomic purposes.
Recently, our group has applied carbon-coated iron nanoparticles to pumpkin plants in order to develop tools for the directed release of chemicals into plant organs susceptible to infection by pathogens that specifically attack them . Using different microscopy methodologies to monitor their presence in plant tissues, we have shown that these nanoparticles penetrate living plant tissues. But as the foregoing observations are only a first step in the directed distribution of nanoparticles in living plants, to what extent these particles are capable of migrating properly to reach their target has yet to be established
The aim of this work was to analyse the penetration and movement of nanoparticles in plant cells, and the capacity of a magnetic field to retain them in specific parts of the plant. Two different application methods were used: injection, and spraying, the latter being a more practical option from an agronomic standpoint. Correlative microscopy, on both light and electron microscopy levels, was used as a convenient experimental methodology, providing evidence of intracellular localization of nanoparticles and their displacements from the application points. Long range movement of particles through the plant body were also detected, these particles being found near one of the magnets used to immobilize and concentrate them.
Plant material and growth conditions
Pumpkin (Cucurbita pepo L.) plants were selected for preliminary experiments due to their large vessel size, which would facilitate the transport of nanoparticles through the vascular system. Plants were grown using a polyethylene bag system as previously described [11, 12]. A strip (11 × 28 cm) of glass fibre paper (Whatmann GF/A) was inserted into a polyethylene bag (25 × 35 cm) as physical support for the development of the root and as a way for the nutrient solution to rise by capillary action and reach the whole root. Pumpkin seeds were germinated in Petri dishes with moistened filter paper. Fifteen day old pumpkin seedlings having root lengths of about 4–5 cm were transferred to the bag, placing them on the upper side of the system. Twenty ml. of Hoagland nutrient solution  was added to each bag, and refilled later when necessary. The bags were suspended vertically in boxes and the plants were grown in a controlled environment chamber with a day/night temperature of 20 ± 0.5 8C, a 14 h photoperiod and a photosynthetic photon flux density of 200 μmol m-2 s-1.
Some nanoparticle-treated plants and some control plants were placed in pots to evaluate whether the treatment would have any effect on the subsequent growth of the plants.
Carbon-coated iron nanoparticles were produced by the discharge arc method [14, 15] based on that previously designed by Krätschmer-Huffman . The Krätschmer-Huffman method uses a cylindrical chamber, in which there are two graphite electrodes: a stationary anode containing 10 μm starting iron powders, and a moveable graphite cathode. An arc is produced between the graphite electrodes in a helium atmosphere. The graphite electrode is sublimed and builds up a powder deposit (soot) on the inner surface of the chamber. It was in this material that we found: carbon nanostructures, amorphous carbon and iron, and iron oxide nanoparticles encapsulated in graphitic layers, together with a small amount of non-coated or partially coated metallic particles.
These particles (which are not biocompatible) were eliminated by chemical etching, washing the soot with 3 M HCl at 80°C. This procedure favours the formation of carboxylic groups on the graphitic shell, which, due to their hydrophobic nature, contribute to the stability of the final particle suspension. In order to eliminate the amorphous carbon and thereby increase the concentration of magnetic nanoparticles, the powder underwent magnetic purification. To do this, stable suspensions of the particles were prepared in a surfactant solution: 2.5 g of SDS in 500 ml of distilled water. A field gradient produced by a permanent magnet (maximum magnetic field 3 KOe) was used for magnetic separation of this suspension. Employing this method, only the carbon-coated magnetic nanoparticles are attracted by the magnet whereas the amorphous carbon remains in the supernatant, which is subsequently eliminated.
Nanoparticles were suspended in gelafundine, a commercial succinated gel (30 g/l gelatine, 0.45% w/v sodium chloride, 0.21 g/l calcium, Braun, Melsungen-Germany), and the solution was kept in water in an ultrasonic bath for several minutes. This solution was selected because it had been used as a convenient suspension medium for nanoparticles in experiments with animals. A biocompatible magnetic fluid was obtained in this way, which was used for the inoculation of plants.
Collection and processing of samples for microscopy analysis
Stem and petiole samples from selected positions on the plant were collected 24, 48 and 168 hours after application of the bioferrofluid and processed for microscopic analysis as previously described . The selected positions (shown in fig. 1B) were the application points and points situated close to the magnets, before and after them, according to the movement that the particles were supposed to follow. They were fixed in Karnovsky solution (4% formaldehyde + 5% glutaraldehyde, in 0.025 M cacodilate buffer, pH 6.9) for 4 h at room temperature, and then kept in the buffer at 4°C.
Approximately 1 mm-thick, hand-cut cross sections of the fixed samples were dehydrated through an ethanol series, infiltrated and embedded in Epon resin (Serva, Heidelberg, Germany).
Microscopy analysis methods
For correlative microscopy, 1–2 μm sections were cut from the polymerised blocks, observed on a light photomicroscope (Leitz, Germany) under phase contrast, bright field and dark field to identify the presence of nanoparticle aggregates as previously described  and photographed using an Olympus DC10 digital camera. The regions of interest were trimmed and 70–100 nm ultrathin sections were obtained. The sections were counterstained with 5% uranyl acetate for 30 min, rinsed in bidistilled water, dried and observed on a transmission electron microscope Jeol 1010 at 80 kV. Micrographs were taken with a Megaview digital camera (Gatan).
Correlative microscopy was used to score the presence of nanoparticles in samples collected from different positions, close to the application points and magnet locations. Following the procedures previously established , a first level of analysis was performed by examining 1–2 μm Epon sections on the light microscope in search of nanoparticles. Aggregates of nanoparticles appear as dark, optically dense signals under bright field, that correlate with a refringent signal under dark field and phase contrast . To make a precise identification of the presence of nanoparticles by light microscopy, their presence was only considered as proven when the three microscopies employed (phase contrast, bright field and dark field) provided correlated images (see additional files 1, 2, 3: correlative images 1, 2, 3 for the micrographs used to select the nanoparticle-containing cells to be analyzed at TEM). For correlative microscopy, those areas that appeared to contain nanoparticles were selected for analysis under TEM.
Two different application methods were employed, namely, injection and spraying (see MM). Plant samples were collected 24 h, 48 h and 168 h after application, these time periods having been previously determined to be appropriate for detectingt internalization of nanoparticles into the tissues .
Internalization of nanoparticles close to the application point
a. Application by injection
b. Application by spraying
Internalization of nanoparticles in locations far from the application point
To evaluate whether the application of nanoparticles had any effect on the subsequent growth of the plants, some treated plants were planted in pots and kept under controlled conditions until the flowering period. No apparent differences were observed between plants subjected to the treatment and the control plants, the former even being able to produce fruit which indicated that treatment with nanoparticles did not provoke a toxicological effect on plant growth. At tissue level, the structural organization of the stem samples of treated and non-treated plants was similar, with no signs of damage at light and electron microscopy levels. Only the cells containing the nanoparticle agglomerates exhibited more dense cytoplasms, as previously stated. This fact suggested that the penetration of nanoparticles through the tissues did not damage them.
In the present work, intracellular penetration of carbon-coated iron nanoparticles applied in planta was tracked using correlative microscopy, including a first screening of the samples with optical microscopy (bright field, dark field and phase contrast) followed by analysis through transmission electron microscopy. This strategy has allowed us to confirm both the progressive penetration of particles through the plant tissues and their presence in the form of intracellular aggregates, when injected into tissues near the application site, in as short a time as 48 h. Furthermore, 48 h after injection, isolated nanoparticles in the cytoplasm of individual cells close to a vascular core were observed in the proximity of a magnetic device located far from the application point. When applied by spraying, isolated nanoparticles were also found in the cytoplasm of epidermic cells, in regions near the application point.
Cell and tissue response to the presence of nanoparticles
Nanoparticles move away from the application point, on the interior of the stem, where they were injected, to the outer epidermis through the tissues. The fact that a dense cytoplasm with starch-containing organelles was observed concomitantly with nanoparticle aggregates in the cytosol, suggests that plant cells could respond to the presence of a high density of nanoparticles by changing their subcellular organization. Results also showed that the nanoparticles had already appeared in the outer surface of the plant, both inside and outside of the trichomes, 24 h after application, indicating that at least part of the bioferrofluid can be expelled in a short time. Several examples of cytotoxicity of carbon nanoparticles have been described in various animal systems [18, 19, 20, 21, 22, 23] as well as different effects derived from the application of magnetic ferrofluid [24, 25]. Cytotoxicity has also been associated with the dose of nanoparticles . This correlation between the number of nanoparticles and cytotoxicity agrees with our results, which showed that no subcellular rearrangements were detected in those plant cells in which only a few isolated nanoparticles were detected (Figs. 8 and 9), compared with the response detected in cells carrying aggregates of nanoparticles. But, to date, there has been no description of changes in cell architecture and organization after nanoparticle internalization in vivo, so it is not possible to assess if the observed reaction is specific to our material or if it is a general trait of cell reaction to high concentrations of nanoparticles within the cell. Moreover, the solution used for nanoparticle suspension, namely gelafundine, contains calcium, an important second messenger in plants. The possibility that some calcium remains attached to the carbon coat of the nanoparticle and could have an effect on cell response, does not seem to be very plausible due to the chemical properties of the nanoparticle surface (hydrophobic, not negatively charged...), even so, it cannot be completely excluded.
Internalization of nanoparticles near the application point
The observation of particle aggregates in adjacent parenchymatic cells suggests a movement from cell to cell. Another question to be addressed is the presence of nanoparticles as intracellular aggregates, as it seems quite unlikely that nanoparticles have the capability of entering cells other than as individual particles. The mechanism involved in this aggregation is unclear. As indicated in MM, the carbon-iron nanoparticles employed show a tendency to aggregate in aqueous solutions because of the chemical characteristics of the carbon coat. Aggregation of nanoparticles is a common phenomenon , so it is possible that this reclustering takes place spontaneously after individual internalization into the cytoplasm, although it could not be discarded that the nanoparticles were redirected by the cell to a specific subcompartment or region in the cytoplasm.
The presence of nanoparticles in epidermal cells after application by spraying is of special interest. As stated before, one of the main drawbacks of other methods is that they cannot be employed for agronomic purposes. The method used in this work resembles the procedures which would be used by breeders and coordinators of phytosanitary control, employing both large scale and hand-on spraying to leaf surfaces. The fact that nanoparticles passed through the epidermal cell wall opens up the possible application of these nanotechnology tools for agronomical purposes. Given the special characteristics of the epidermic outer cell wall, specifically its considerable thickness, and the presence of protective waxes, a possible particle penetration point could be through the stomata and the subestomatic chambers. In fact, this aperture is a route used by pathogens of different species, such as the white pine blinter rust . Interestingly, water-suspended 43 nm hydrophilic particles have been described as occasionally penetrating Vicia faba leaves through stomatal pores . Recently, the uptake of magnetite nanoparticles through the root system of Cucurbita maxima plants was reported, though a significant uptake of particles was only found in plants growing in liquid media . So, in order to make the system suitable for agronomical purposes, methodological improvements would need to be made.
Long range transport and putative movement through the vascular system
The main reason for developing the carbon-iron nanoparticles and their application in this work was to accumulate them at a specific site in the plant by means of magnetic fields applied specifically to a certain area. As shown in Fig. 9, isolated nanoparticles were detected in the cytoplasm of cells close to the vascular core, far from the application point and near the magnet. The position of these cells suggests that the route taken by the particles involved the use of the plant vascular system. Direct observation of freshly cut material revealed the presence of bioferrofluid, specifically in the interior of the xylem vessels , along with nanoparticle aggregates, 48 h after application (Fig. 7) suggesting that the particles can use this system for long range transport. It has to be noted that those particles found far from their application point (Fig. 9) were quite homogeneous in size, around 46 nm in diameter on average, when compared with the variable sizes found in the original mixture detected in the aggregates in the pith cavity and cells close to the application point. (Figs. 2, 3, 4, 5, 6, 7). This may suggest that a certain critical size is required for the appropriate movement of particles through the plant by long range transport mechanisms.
Taken together, the results reported here demonstrate that the carbon-iron nanoparticles are able to get into the cells of living plants, and move to remote positions, possibly through the vascular system. These facts constitute an important step forward in elucidating the mechanisms of interaction between plant cells and nanoparticles and thus, in designing strategies for using nanoparticles for targeted delivery of substances. In this sense, methodological improvements are required to make the system suitable for agronomical purposes, one of them being the functionalization of the nanoparticles with organic groups that can help their penetration into the phloem or make their internalization into cells more efficient. The evidence reported here, that nanoparticles were able to move to locations where the magnets were located, far from the application point, would suggest the potential use of carbon-coated nanoparticles for directed delivery of substances for phytosanitary purposes, using magnetic fields to retain the particles in areas of interest.
This research was supported by the project NanoAgro-200540F0041 funded by CSIC (Proyectos Intramurales de Frontera) and by the project NAN2004-09270-C03-03 funded by the Spanish Strategic Action on Nanoscience and Nanotechnology of the Spanish Ministry of Education and Science. The work was also partially supported by projects granted by the Spanish Ministry of Science and Innovation, MICINN, BFU2008-00203, AGL2008-04255 and AGL2008-01467. JMF thanks ARAID Foundation for financial support.
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