Atypical DNA methylation of genes encoding cysteine-rich peptides in Arabidopsis thaliana
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In plants, transposons and non-protein-coding repeats are epigenetically silenced by CG and non-CG methylation. This pattern of methylation is mediated in part by small RNAs and two specialized RNA polymerases, termed Pol IV and Pol V, in a process called RNA-directed DNA methylation. By contrast, many protein-coding genes transcribed by Pol II contain in their gene bodies exclusively CG methylation that is independent of small RNAs and Pol IV/Pol V activities. It is unclear how the different methylation machineries distinguish between transposons and genes. Here we report on a group of atypical genes that display in their coding region a transposon-like methylation pattern, which is associated with gene silencing in sporophytic tissues.
We performed a methylation-sensitive amplification polymorphism analysis to search for targets of RNA-directed DNA methylation in Arabidopsis thaliana and identified several members of a gene family encoding cysteine-rich peptides (CRPs). In leaves, the CRP genes are silent and their coding regions contain dense, transposon-like methylation in CG, CHG and CHH contexts, which depends partly on the Pol IV/Pol V pathway and small RNAs. Methylation in the coding region is reduced, however, in the synergid cells of the female gametophyte, where the CRP genes are specifically expressed. Further demonstrating that expressed CRP genes lack gene body methylation, a CRP4-GFP fusion gene under the control of the constitutive 35 S promoter remains unmethylated in leaves and is transcribed to produce a translatable mRNA. By contrast, a CRP4-GFP fusion gene under the control of a CRP4 promoter fragment acquires CG and non-CG methylation in the CRP coding region in leaves similar to the silent endogenous CRP4 gene.
Unlike CG methylation in gene bodies, which does not dramatically affect Pol II transcription, combined CG and non-CG methylation in CRP coding regions is likely to contribute to gene silencing in leaves because loss of this methylation in synergid cells is associated with CRP gene expression. We discuss this unusual methylation pattern and its alteration in synergid cells as well as the possible retrogene origin and evolutionary significance of CRP genes that are methylated like transposons.
KeywordsGene Body Female Gametophyte Gene Body Methylation Synergid Cell SAT5 Repeat
Arabidopsis Genome Initiative
- ATSAT5 (SAT5)
Arabidopsis thaliana satellite sequence on chromosome 5
Decrease in dna methylation1
Defective in meristem silencing 4
Domains rearranged methyltransferase
Early culture abundant
Green fluorescent protein
Methylation-sensitive amplification polymorphism
Largest subunit of nuclear RNA polymerase IV (Pol IV)
Largest subunit of nuclear RNA polymerase V (Pol V)
RNA-DEPENDENT RNA POLYMERASE 2
RNA-DEPENDENT RNA POLYMERASE 6
Short name for ATSAT5
The Arabidopsis Information Resource.
Plants have evolved a complex transcriptional machinery for generating and using small RNAs that guide DNA cytosine methylation at homologous regions of the genome. Key components of the RNA-directed DNA methylation pathway include two functionally diversified RNA polymerase II (Pol II)-related RNA polymerases called Pol IV and Pol V . Pol IV is needed to produce the small RNA trigger for methylation whereas Pol V acts downstream of small RNA biogenesis to facilitate de novo methylation of genomic DNA at the small RNA-targeted site. Additional factors, including chromatin remodelers, putative transcription factors, and several novel, plant-specific proteins whose functions are not well understood, are required for Pol V function [1, 2]. In a current model, Pol V synthesizes scaffold transcripts that interact with ARGONAUTE4-bound small RNAs, which recruits the methylation machinery to the target DNA .
RNA-directed DNA methylation results in a characteristic modification pattern that is typified by methylation of cytosines in all sequence contexts (CG, CHG and CHH, where H is A, T or C) within the region of small RNA-DNA sequence homology . In particular, asymmetric CHH methylation is a hallmark of RNA-directed DNA methylation. DOMAINS REARRANGED METHYLTRANFERASE2 (DRM2) is the major enzyme catalyzing de novo methylation of cytosines in all sequence contexts in response to small RNA signals [5, 6]. The maintenance activities of METHYLTRANSFERASE1 (MET1) and CHROMOMETHYLASE3 act primarily to perpetuate pre-existing CG and CHG methylation, respectively, during successive rounds of DNA replication .
Transposons, pseudogenes and non-protein coding repeats are frequent targets of RNA-directed DNA methylation [8, 9]. By contrast, protein coding genes are generally free of RNA-directed DNA methylation unless intimately associated with repeats or transposon-related sequences [10, 11, 12]. However, up to 30 % of expressed genes in Arabidopsis thaliana have in their gene bodies exclusively CG methylation that relies on MET1 and is independent of the RNA-directed DNA methylation pathway [8, 9]. Thus, transposons and genes can be distinguished by discrete methylation patterns that are imposed by different methylation machineries. The origins of these distinct methylation patterns and their functional significance are not yet fully understood [13, 14, 15]. The biological role of CG methylation in gene bodies, which does not inhibit transcriptional elongation by Pol II, is unknown but it may prevent spurious transcription from internal promoters  or help to define exons . An alternative proposal is that gene body methylation restrains genes from being responsive to internal or external cues, e.g. developmental or environmental signals .
Curiously, even though transposons are frequent targets of RNA-directed DNA methylation, only a small subset of transposons is selectively reactivated in mutants defective in this epigenetic pathway [15, 19]. By contrast, a number of transposons are mobilized in mutants defective in MET1 or the chromatin remodeler DECREASE IN DNA METHYLATION1 [13, 20]. Therefore, even though RNA-directed DNA methylation contributes to repression of transposons it is not the sole epigenetic modification involved in the silencing of these elements [13, 15].
In a search for targets of RNA-directed DNA methylation in Arabidopsis, we identified several members of a gene family encoding small cysteine-rich peptides (CRPs). These CRP genes are atypically methylated like transposons, containing CG, CHG and CHH methylation in their gene bodies. This gene body methylation, which is due in part to RNA-directed DNA methylation, is associated with gene silencing in leaves and is reduced in synergid cells of the female gametophyte where the CRP genes are specifically expressed. Here we report these results and discuss the possible origin and evolutionary significance of this unusual pattern of DNA methylation.
Atypical methylation pattern of CRP genes embedded in SAT5 repeats
Single dispersed copies of SAT5 repeats
CRPgene embedded(AGI number)
transposon and gene
Methylation of CRP coding regions: Pol IV/Pol V-dependent CHH methylation
Percent methylation of individual cytosines in SAT5 repeat monomer containing the CRP4 gene
3/3 (100 %)
6/6 (100 %)
22/51 (43 %)
29/51 (57 %)
11/11 (100 %)
20/20 (100 %)
44/52 (85 %)
8/52 (15 %)
2/2 (100 %)
1/1 (100 %)
19/28 (68 %)
9/28 (32 %)
The CRP4 gene is not exceptional in its methylation pattern. Several other predicted protein-coding genes in the CRP3600 family, such as At3g42565 (embedded in a SAT5 repeat; Table 1) and At5g42895 (CRP5a) and At5g44495 (CRP5b), which are not embedded in SAT5 repeats (Additional file 3: Table S1), also contain in their coding regions dense CG, CHG and CHH methylation http://neomorph.salk.edu/epigenome/epigenome.html. This methylation is due in part to RNA-directed DNA methylation as evidenced by the loss of non-CG methylation in a drm1 drm2 cmt3 (ddc) triple mutant http://neomorph.salk.edu/epigenome/epigenome.html, which isdefective in the de novo methyltransferase DRM2 that is required for RNA-directed DNA methylation [5, 6].
Unique Pol IV-dependent 24-nt small RNAs originating from CRP coding regions
Expression of CRP genes in the female gametophyte
To obtain a more detailed view of the cell type-specific expression pattern, we generated constructs encoding a GUS reporter protein and a CRP4-GFP fusion protein, both under the control of a CRP4 promoter fragment, which comprises 921 bp upstream of the ATG start codon of the CRP4 gene. After introducing these constructs into wild-type Arabidopsis plants, GUS and CRP4-GFP gene expression was observed specifically in the synergid cell region of the female gametophyte, indicating that the CRP4 promoter is active exclusively in these cells (Figure 6B). We therefore infer that the detection of CRP4 transcripts in flowers of non-transgenic plants (Figure 6A) reflects expression of the endogenous CRP4 gene in synergid cells.
CRP4 coding sequence is not methylated when transcribed by Pol II to produce a translatable mRNA
We obtained further evidence that the CRP4 coding sequence is unmethylated when transcribed by Pol II by examining methylation of a CRP4-GFP fusion gene under the control of the constitutive 35 S promoter in leaves. In transgenic plants containing this construct, the CRP4 coding sequence of the CRP4-GFP fusion gene remained largely unmethylated (Figure 8A, left). Moreover, a CRP4-GFP fusion protein was detectable on Western blots in these plants, indicating Pol II transcription of a translatable CRP4-GFP mRNA from the 35 S promoter (Figure 8B, lanes 1–4). By contrast, the CRP4 coding sequence of a CRP4-GFP fusion gene under the control of the CRP4 promoter acquired methylation similar to the endogenous CRP4 gene (Figure 8A, right) and no CRP4-GFP fusion protein was detected in plants containing this construct (Figure 8B, lanes 5,6). Thus, CRP4 coding sequences transcribed by Pol II lack methylation in both synergid cells, where the endogenous CRP4 gene and CRP4-GFP fusion gene are expressed from the CRP4 promoter, and in leaves, where the fusion gene is under the control of the constitutive 35S promoter.
The CRP4 gene is not inducible by flg22 or heat treatment in seedlings
Current views of DNA methylation patterns in plants hold that gene body methylation occurs primarily in a CG context and that non-CG methylation is limited to transposons and non-protein-coding repeats [13, 32]. Contrary to this view, we have identified several genes encoding CRPs in Arabidopsis that atypically contain in their gene bodies not only CG but also CHG and CHH methylation, which arises in part from RNA-directed DNA methylation. These genes are thus methylated like transposons in their coding regions. Evidence for this claim includes the detection of Pol IV-dependent 24-nt small RNAs that are unique to each gene and the reliance of full CHH methylation on components of the Pol V pathway. In addition to RNA-directed DNA methylation, other methylation pathways contribute to the dense methylation of CRP gene bodies in leaves. This is indicated by the persistence of nearly wild-type levels of CG and CHG methylation in mutants defective in RNA-directed DNA methylation, which lose predominantly CHH methylation. Multiple layers of epigenetic modifications have been observed previously for other targets of RNA-directed DNA methylation in plants [13, 15, 32, 33]. The biological role of the transposon-like methylation pattern of CRP gene bodies is not yet clear but it is likely to be related to gene silencing in the sporophyte. As discussed below, alternative functions can also be considered.
The CRP genes investigated in this study are silent and methylated in leaves and, given the restricted expression pattern of the CRP4 promoter when fused to GUS and GFP reporter genes, presumably in other sporophytic tissues as well. As shown here and elsewhere , the promoters of many CRP genes in the CRP3600 family are active specifically in the egg apparatus region of the female gametophyte (i.e. egg and synergid cells). The functions of CRPs in the female gametophyte are not yet clear but possibilities include acting as pollen tube attractants , antimicrobial substances in the vulnerable egg cell region [29, 35, 36], or intercellular signaling molecules during female gametophyte development [37, 38, 39]. In our experiments, the synergid cell-specific expression of the CRP4-GFP fusion gene, and presumably the CRP4 endogenous gene, was correlated with a loss of CG and CHH methylation in the CRP4 gene body at the restriction sites tested. This finding suggests a connection between the observed gene body methylation and CRP4 gene silencing in leaves. We assume a similar association holds true for the CRP5a and CRP5b genes, which are highly methylated in their gene bodies in leaf tissue http://neomorph.salk.edu/epigenome/epigenome.html yet expressed exclusively in the egg apparatus region of the female gametophyte .
The influence of gene body methylation on expression depends on the sequence context of methylated cytosines. In wild-type plants, CG methylation alone in gene bodies does not inhibit transcriptional elongation and is found at many moderately expressed genes [8, 9, 13, 16]. CHG methylation in gene bodies, which is induced in the ibm1 (increase in BONSAI methylation1) mutant, has variable effects on transcription, with only some target genes showing decreased transcription while others are unchanged or even more highly expressed . By contrast, our results suggest that a combination of dense CG and non-CG methylation in gene bodies is associated with gene silencing. Whether this methylation inhibits transcriptional elongation by Pol II or is the default state when transcription is impaired by other means is not yet known. Also unclear is how methylation in CRP4 gene bodies is lost in synergid cells. It may result from active demethylation by DNA glycosylase activities, as observed for imprinted genes in the central cell of the female gametophyte [41, 42], or a lack of silencing factors in synergid cells or their precursors. A recent global enrichment study demonstrated that genes encoding components of the RNA-directed DNA methylation machinery, in particular six PAZ-domain proteins, are predominantly expressed in egg cells but some are also expressed in synergid cells . Loss of CRP4 gene body methylation in synergid cells may also reflect the restricted cellular distribution of essential transcription factors, such as the synergid cell-specific transcription factor MYB98 [27, 44]. However, there does not seem to be a correlation between methylation of CRP genes in leaves and a requirement for MYB98 in synergid cell-specific expression. For example, both CRP5a and CRP5b are methylated in leaves and expressed in synergid cells but only the former requires MYB98 .
Although gene silencing seems the most likely function and/or consequence of combined CG and non-CG methylation in the CRP gene bodies, a possible positive role can also be considered, namely that gene body methylation attunes genes to internal or external cues. This suggestion follows from recent data demonstrating that gene body methylation, whether in a CG or non-CG context, can be dynamic and may render genes sensitive to developmental or environmental signals. For example, a bioinformatics study examining genome-wide gene expression in Arabidopsis under many different experimental conditions concluded that CG methylation in gene bodies protects genes from reacting to internal or external stimuli . In tomato, a drought-responsive gene that atypically has non-CG methylation in the gene body loses non-CG methylation and gains CG methylation under drought conditions . Some transcribed genes in human embryonic stem cells contain in their gene bodies non-CG methylation that is lost as cells differentiate and re-established during de-differentiation, suggesting a relationship between non-CG methylation in gene bodies and the differentiation state of the cells [14, 46]. In view of these findings, it is conceivable that combined CG and non-CG methylation in CRP gene bodies allows responsiveness to either developmental cues in the female gametophyte or external factors in the environment. Regarding the latter possibility, we did not observe de-repression of the methylated CRP4 gene in leaf tissue following treatment with flg22 or heat stress, but other biotic or abiotic stresses can be tested in the future.
Why are the gene bodies of some CRP3600 gene family members methylated like transposons? Although several methylated CRP genes are embedded in SAT5 repeats this is not the case for all (for example, CRP5a and CRP5b), demonstrating that the presence of the flanking SAT5 sequences is not a requirement for methylation. To explain the dense methylation concentrated in the CRP coding regions, we propose that some members of the CRP3600 gene family arose through reverse transcription of mature mRNAs (retroposition) and hence can be classified as retroposed genes (retrogenes). Under this hypothesis, an intron-containing progenitor copy would have been highly expressed in the egg cell or one of its precursors in the germline, the spliced mRNA reverse transcribed, and the cDNA subsequently integrated into egg cell DNA to become a heritable part of the genome. Through this copying and integration process, CRP retrogenes would resemble transposons and be methylated accordingly in their coding regions. Consistent with this hypothesis, only one gene in the CRP3600 family has two introns (At5g53905) while the rest are intron-free and thus candidate retrogenes (Table 2). The observation that at least some CRP3600 gene family members are probably expressed in the egg cell as well as the neighboring synergid cells  is compatible with the retrogene hypothesis. Interestingly, retrogenes in mammals are expressed mainly in the testes, where transcription is less tightly regulated than in somatic tissues . Whether controls on transcription are similarly relaxed in synergid cells and/or egg cells in plants remains to be determined.
Not all copies of the CRP3600 gene family have a transposon-like methylation pattern in their gene bodies. Some lack methylation entirely and one has CG methylation only (Additional file 3: Table S1). Although the source of such differences is presently unknown, one possibility is that recently integrated copies still have the initial transposon-like methylation pattern, which over time degenerates into exclusively CG methylation, ultimately resulting in the complete decay of gene body methylation. Because methylated cytosine spontaneously deaminates to produce thymine, methylated CRP coding regions may experience accelerated sequence diversification over evolutionary time, thus promoting functional diversification of CRP proteins. Given known or proposed roles of CRPs in pollen tube guidance  and self-incompatibility [38, 48], such sequence changes and functional diversification may help to drive speciation.
We have identified several genes of the Arabidopsis CRP3600 subgroup that have an unusual transposon-like methylation pattern consisting of CG, CHG and CHH methylation in their gene bodies. This methylation, which was observed in leaves, appears to be associated with gene silencing in sporophytic tissues because it decreases in synergid cells of the female gametophyte, where the CRP genes are specifically expressed. Analysis of CRP3600 gene family members suggests that at least some methylated copies may have arisen through retroposition, causing them to be epigenetically modified like transposons through RNA-directed DNA methylation and other methylation pathways that target mobile elements. The CRP genes will be useful models for studying developmentally-regulated changes of combined CG and non-CG methylation in gene bodies and the expansion through retrotransposition of gene families expressed in the plant germline.
All experiments have been performed using Arabidopsis thaliana accession Col-0. For the mutants defective in RNA-directed DNA methylation the alleles used were drd3-7 (for nrpe1) , rdr2-1 (SAIL_1277H08; , nrpd1-7 (formerly nrpd1a-7; ), dms4-1, dcl3-1 (SALK_005512; ) and rdr6-1. Mutants were genotyped using primers shown in Additional file 4: Table S3. Plants were cultivated on potting soil in either a greenhouse or growth chamber set at ~ 21°C with a 16 hour light/8 hour dark cycle.
Constructs and plant transformation
For expression studies, three constructs were made: 35Spromoter-CRP4-EGFP CRP4promoter-CRP4-EGFP, and CRP4promoter-GUS. To assemble the constructs, the CRP4 promoter region (consisting of 921 bp upstream of ATG start codon) and the CRP4 coding region (369 bp) were modified with restriction enzyme sites that are necessary for the plasmid construction [modified sequence synthesized by Mr.Gene (Regensburg, Germany)]. For construction of the CRP4-GFP fusion gene, a sequence encoding enhanced green fluorescent protein (EGFP, referred to hereafter as GFP) was added to the C-terminus of CRP4 (including both promoter and coding region). We also fused the same GFP coding sequence to the C-terminus of the CRP4 coding region, which was driven by the cauliflower mosaic virus 35 S promoter. Both of these fragments were inserted into a pPZP221 binary vector , which has a gentamicin selection marker driven by the 35 S promoter  for selection of transformed plants. To construct the ß-glucuronidase (GUS) reporter under the control of CRP4 promoter, a GUS coding sequence (plasmid pRT102)  was inserted downstream of the CRP4 promoter and introduced into pPZP221 binary vector .
All three plasmids (pPZP 35Spromoter-CRP4-GFP, pPZP CRP4 promoter-CRP4-GFP, and pPZP CRP4promoter-GUS ) were transferred into Agrobacterium tumefaciens strain ASE using triple mating  and transgenic plants were obtained by using the floral dip method .
Methylation-sensitive amplification polymorphism (MSAP)
MSAP analysis was performed according to a published procedure  with some modifications. Genomic DNA was isolated using the Qiagen DNAeasy Maxi Kit (Qiagen, Hilden, Germany) and 1 μg of genomic DNA was digested with EcoT22I/MseI overnight. Fifty pMol MseI adapters and 5 pMol PstI adapters were added and ligation was performed for 3 hrs at 37°C. Sterile H2O up to 500 μl was added and the samples were stored at - 20°C until use. Adapters were prepared by adding equimolar amounts of both strands.
For PCR reactions, the EcoT22I primer was radioactively labeled using [γ32P] ATP and T4 polynucleotide kinase (reaction carried out on ice) (Roche, Vienna, Austria). The following PCR program was used: 1 cycle: 1 minute 94°C, 30 seconds 65°C, 1 minute 72°C afterwards the annealing temperature was lowered each cycle by 0.7°C during 12 cycles. This gave a touch down phase of 13 cycles. This was followed by: 23 cycles: 30 seconds 94°C, 30 seconds 56°C, 1 minute 72°C; 10 minutes 72°C; ∞ 4°C. PCR products were electrophoresed on 5 % polyacrylamide-7.5 M urea-gels [acrylamide:bis-acrylamide, 19:1, (Roth, Karlsruhe, Germany]. Pre-electrophoresis was run at 60 Watt for 30 minutes in 1 x TBE running buffer until plates were at approximately 45°C. Electrophoresis was performed for 2 hours 30 minutes at 60 Watt until xylene cyanol was two-thirds down of the length of the gel. Gels were blotted onto 3MM Whatman filter paper covered with Saran Wrap and dried in the gel dryer for 2 hours 30 minutes at 80°C. The gels were exposed to Kodak BioMax MS Film overnight at room temperature.
Bands showing polymorphisms were cut out from the 3MM Whatman filter paper. 100 μl sterile H2O was added and the bands were incubated o/n at 4°C. DNAs were reamplified and cloned into the pGEM-T Easy vector (Promega, Vienna, Austia). The following PCR program was used for re-amplification: 95°C 2 minutes; 95°C 10 seconds, 56°C 20 seconds, 72°C 1 minute 30 seconds, 35 cycles; 72°C 7 minutes. Cloned fragments were re-run on an MSAP gel side by side with the genomic DNA fragments to make sure that the correct fragment was cloned and to check the reproducibility of the polymorphism. Robust polymorphic bands were sequenced.
Primers used to detect and clone the chromosome 2 SAT5 copy were T21 (5′-GAC TGC GTA GGT GCA TCC-3′) and M56 (5′-GAT GAG TCC TGA GTAATG T-3′). For the chromosome 5 SAT5 copy, the primers used were T21 (see above) and M1 (5′GAT GAG TCC TGA GTA ATA-3′).
Genomic DNA was isolated from rosette leaves using a DNeasy Plant Maxi kit (Qiagen, Hilden, Germany). Bisulfite treatment of DNA was conducted using a EpiTect Bisulphite kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions with several modifications as described previously . The PCR reactions were performed using Advantage 2 Polymerase Mix from Clontech (Mountain View, California) and the conditions for the amplification of bisulfite-treated DNA were as follows: 94°C for 2 min followed by 34–39 cycles at 94°C for 30 sec, 30 sec annealing temperature for a particular primer pair (see Additional file 4: Table S3), 68°C for 45 sec, and 6 min of final elongation. The PCR was carried out in a total reaction volume of 50 μl. As a control for complete bisulfite conversion, we used the PHAVOLUTA gene : PCR conditions are the same as above except for the first pair of primers we used 40 cycles and for the second pair 35 cycles. Primers are shown in Additional file 4: Table S3. The 1012 bp SAT5 copy containing the CRP4 gene was analyzed in three overlapping parts. The results shown in Figure 2 are from sequencing of at least 15 clones of each part in wild-type and mutant plants.
Small RNA isolation and Northern blot analysis
Small RNAs were isolated from pooled 21-day-old Arabidopsis seedlings or inflorescence tissues using the mirVana miRNA isolation kit (Ambion/Applied Biosystems, Brunn am Gebirge, Austria) and analysed by Northern blot hybridization according to a published procedure [23, 33, 58]. We used an end-labeled oligonucleotide probe to detect tasiRNA255 (5′-TAC GCT ATG TTG GAC TTA GAA-3′) and an end-labeled LNA (locked nucleic acid) oligonucleotide (5′-AAL CAA LAA TLG TTL GGT LCA TLC CGL TGA LTG GLA TAL TTC LCT GLG ATL GCA-3′ as a probe to detect bulk SAT5 small RNAs.
Small RNA sequencing
Libraries of small RNAs were constructed from total RNA isolated from mixed stage inflorescence tissues of wild type (wt) Arabidopsis Col-0, mutants nrpd1 and rdr2 using TRIzol reagent (Invitrogen). Total RNA (200 μg) for each sample was used to construct the small RNA librarires as described previously but with different adapters. The RNA oligos (Dharmacon) used for small RNA ligations were as follows: 5′ RNA Adapter (5′OH-GUUCAGAGUUCUACAGUCCGACGAUC-OH 3′) and 3′ RNA Adapter: (5′ pUCGUAUGCCGUCUUCUGCUUGUidt 3′; p, phosphate; idT, inverted deoxythymidine). Libraries were sequenced on an Illumina HiSeq2000 at the Delaware Biotechnology Institute. This generated 12,043,425 total reads from wt Col-0, 13,580,575 from mutant nrpd1 and 9,001,733 from mutant rdr2.
Adapter sequences were removed using a Perl script, generating small RNA sequences plus abundances. The data were matched to the Arabidopsis genome (TAIR v9). Of the 12,043,425 total reads from wt Col-0, 13,580,575 from mutant nrpd1 and 9,001,733 from mutant rdr2, 8,577,577 (wt), 2,858,557 (nrpd1) and 4,322,052 (rdr2) reads matched the genome, excluding 1,709,653 (wt), 8,889,937 (nrpd1) and 3,664,258 (rdr2) that matched tRNA, rRNA, snRNA or snoRNAs. Libraries of small RNAs were constructed from total RNA isolated from mixed stage inflorescence tissues of wild type (WT) Arabidopsis Col-0, mutants nrpd1 and rdr2 using TRIzol reagent (Invitrogen, Carlsbad, CA, USA). Total RNA (200 μg) for each sample was used to construct the small RNA libraries as described previously  but with different adapters. The RNA oligos (Dharmacon, Lafayette, Colorado, USA) used for small RNA ligations were as follows: 5′ RNA Adapter (5′OH-GUUCAGAGUUCUACAGUCCGACGAUC-OH 3′) and 3′ RNA Adapter: (5′ pUCGUAUGCCGUCUUCUGCUUGUidt 3′; p, phosphate; idT, inverted deoxythymidine). Libraries of small RNAs were sequenced using Illumina’s (San Diego, CA, USA) sequencing by synthesis technology at the Delaware Biotechnology Institute. This generated 12,043,425 total reads from wt Col-0, 13,580,575 from mutant nrpd1 and 9,001,733 from mutant rdr2.
Adapter sequences were removed using a Perl script, generating small RNA sequences plus abundances. The data were matched to the Arabidopsis genome (TAIR v9). Of the 12,043,425 total reads from wildtype Col-0 (WT), 13,580,575 from mutant nrpd1 and 9,001,733 from mutant rdr2, 8,577,577 (WT), 2,858,557 (nrpd1) and 4,322,052 (rdr2) reads matched the genome, excluding 1,709,653 (WT), 8,889,937 (nrpd1) and 3,664,258 (rdr2) that matched tRNA, rRNA, snRNA or snoRNAs. The small RNA sequence data are available from NCBI's Gene Expression Omnibus (GEO) and are accessible via GEO Series accession number GSE34207. Accession numbers for the separate libraries are GSM844489 (rdr2_sRNA); GSM844490 (nrpd1 rdr2_sRNA); and GSM844491 [WT (Col-0)_sRNA].
Total RNA was extracted from 3 week old seedlings (100 mg) or mixed floral inflorescences (100 mg) by using TRIzol® Reagent (Invitrogen, Lofer, Austria) according to the manufacturer’s instructions. One μg of total RNA was used for reverse transcription using RevertAid™ H Minus First Strand cDNA Synthesis Kit (Fermantas, St. Leon-Rot, Germany) following the manufacturer’s instructions. One μL cDNA was used for semi-quantitative reverse transcriptase–mediated (RT) PCR analysis. The PCR conditions were 95°C for 5 min followed by 23 (actin), 25 (MPK3), 40 (CRP genes) amplification cycles (95°C for 30 s, 55°C for 30 s, and 72°C for 1 min). Actin (At3g18780) was used a constitutively expressed internal control. Primers used for RT-PCR are listed in Additional file 4: Table S3.
GUS assays and GFP visualization
Transgenic plants transformed with the three constructs described above were used to detect synergid cell-specific expression of GUS and GFP reporter genes. GUS assays were performed as described previously . Briefly, ovules were dissected from early opened flowers (emasculated 24 hr before) in a small petri dish containing GUS staining solution (50 mM sodium phosphate buffer pH 7.0, 10 mM EDTA, 0.1 % Triton X-100, 2 mM potassium ferrocyanide, 2 mM potassium ferricyanide, and 1 mg/ml X-Gluc) on ice. The ovules were incubated at 37°C for 30 min, cleared with 20 % methanol/4 % concentrated HCl solution at 55°C for 15 min, and then transferred into a solution containing 60 % ethanol/1.8 M NaOH at 25°C for 10 min. Samples were washed with 30 % ethanol and 10 % ethanol and mounted onto slides with a 50 % glycerol solution. Images were acquired using a Leica stereofluorescence microscope MZ16FA equipped with a Leica GFP2 filter set and a Leica DFC 300FX camera (Leica GmbH, Germany).
To visualize GFP, ovules were dissected from early opened flowers (before fertilization), and localization of the GFP signal was viewed by using Leica stereofluorescence microscope MZFLIII.
Tissue Embedding and Laser-Assisted Microdissection (LAM)
Flowers were emasculated and harvested 48–72 hrs thereafter. The tissue was fixed in EtOH:Acetic Acid 3:1 (v/v) under vacuum for 2x 15 min at 4°C, according to Kerk and coworkers , then left in the fixative overnight at 4°C. The tissue was embedded in Paraplast® Regular embedding media (Sigma-Aldrich, Vienna, Austria) using an automated protocol.
In the automated procedure, the fixed tissue was embedded using the automated Sakura Tissue-Tek®VIP™ embedding machine (Sakura Finetek Europe B.V., Alphen aan den Rijn, The Netherlands) with the following parameters: 1 hr 70 % EtOH at 37°C, 1x 1 h 80 % EtOH, 2x 1 h 96 % EtOH, 2x 1 h 100 % EtOH, 2x 1 h 100 % Xylol, all at 40°C, and then 4x 1 h Paraplast® Regular embedding media (Sigma-Aldrich, Vienna, Austria) at 60°C. Flowers were poured into paraffin-blocks, cooled and then kept at 4°C until use.
For microdissection, the embedded flowers were sectioned on the Leica microtome RM2145 (Leica Microsystems GmbH, Wetzlar, Germany) to 8 μm thickness and mounted onto RNase-free membrane slides (Molecular Machines & Industries [MMI] AG, Glattbrugg, Switzerland) with the use of RNAse free miliQ water. Slides were dried overnight on a heating table at 42°C and processed the following day. Microdissection was performed with a CellCut Plus instrument (MMI AG, Glattbrugg, Switzerland), which makes use of a solid state UV-A laser (wavelength approx. 350 nm) to cut the tissue. Each slide was deparaffinized by processing in 100 % Xylene for 2x 10 minutes at room temperature. Slides were air-dried for 10 min. and immediately used for laser microdissection. Synergids cells were harvested using MMI isolation caps, with an average of 110 cells isolated per cap. Due to the cytology of the egg apparatus, it was not possible to isolate the synergids cells without including a small basal part of the egg cell in the same section . However care was taken to avoid contamination of the sample with egg cell nuclei. Therefore, for DNA methylation studies the sample can be considered as purely synergid cells.
Detection of CRP4-GFP fusion proteins by Western blotting
Total proteins were extracted from 3 week old seedlings grown on solid Murashige and Skoog (MS) medium. Approximately 50 mg seedlings were harvested, ground in liquid nitrogen and resuspended using 100 μl extraction buffer (50 mM HEPES-KOH pH 7.9, 400 mM KCl, 2.5 mM MgCl2, 1 mM EDTA, 1 mM DTT, 0.1 % Triton X-100) supplemented with EDTA-free protease inhibitor cocktail (Roche, Vienna, Austria). After centrifuging 15 min at 4°C, the supernatant was mixed with the same volume of extraction buffer without KCl. Proteins were separated using 10 % SDS–PAGE and transferred to a PVDF membrane (Millipore, Vienna, Austria). Membranes were probed either with mouse monoclonal antibodies against GFP (1:1,000) (Roche, Vienna, Austria) or tubulin (1:1,000) (Sigma-Aldrich, Vienna, Austria), followed by horseradish peroxidase conjugated goat anti-mouse secondary antibody (1:10,000, Biorad, Vienna, Austria). The blots were developed using an enhanced chemoluminescence ECL kit (Thermo Scientific, Vienna, Austria), and exposed to CL-XP X-ray film (Thermo Scientific, Vienna, Austria).
Treatment with flg22 and heat stress
Dried seeds were sterilised with 70 % ethanol/ 0.05 % tritonX-100 and sown on solid MS medium. One week after germinating, small seedlings were transferred to liquid MS medium supplied with 1 μM flg22 peptide (kindly provided by Elke Logemann and Imre Somssich, Max Planck Institute for Plant Breeding Research, Cologne) (4 seedlings per 400 μl of medium in wells of 24 well plates). Batches of seedlings were collected after 10 min, 20 min, and 30 min of flg22 treatment followed by semi-quantitative RT-PCR to detect expression of the CRP4 gene and MPK3.
For heat stress, approximately one week-old seedlings were transferred into liquid MS medium and incubated at 4°C for 24 hr, and different batches were then incubated for 4 hrs, 8 hrs, and 24 hrs, respectively, at 37°C. Stressed seedlings were allowed to recover at 21°C for 3 days, and then used for semi-quantitative RT-PCR to detect expression of the CRP4 gene and Onsen retrotransposon .
Accession numbers for the single SAT5 monomers containing CRP genes or pseudogenes shown in Table 1 are as follows: chr2 SAT5 fragment containing At2g18042 pseudogene [EMBL:HE610172]; chromosome 3 SAT5 fragment containing At3g42565 gene [EMBL:HE610173]; chromosome 4 SAT5 fragment containing At4g09545 gene [EMBL:HE610174]; chromosome 5 SAT5 fragment containing At5g60978 pseudogene [EMBL:HE610175]. The small RNA sequence data are available from NCBI’s Gene Expression Omnibus (GEO) and are accessible via GEO Series accession number GSE34207.
Work in the Matzke lab is supported by the Austrian Academy of Sciences and a grant from the Austrian Fonds zur Förderung der wissenschaftlichen Forschung (grant no. SFB F4306-B09). Work in the Meyers lab is supported by the US NSF Plant Genome Research Program award #0701745. Work in the Grossniklaus lab is supported by the University of Zürich and the Swiss National Science Foundation. We thank Zdravko Lorkovic for critical reading of the manuscript and Johannes van der Winden for technical and editorial assistance.
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