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BMC Neuroscience

, 11:P49 | Cite as

Phase response curve analysis of network properties in the deep cerebellar nuclei

  • Marylka Uusisaari
  • Benjamin Torben-Nielsen
  • Klaus M Stiefel
Open Access
Poster Presentation

Keywords

Purkinje Cell Network Property Spike Timing Cerebellar Purkinje Cell Deep Cerebellar Nucleus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

The deep cerebellar nuclei (DCN) are the final unit of cerebellar computation, where the excitatory input into the cerebellum is integrated with inhibitory input from the cerebellar Purkinje cells. In some sense the entire state of cerebellar function can be thought of as compressed into the state of DCN network. However, even though the main cell types in DCN have recently been described [1], next to nothing is known about their roles in the DCN network, or even about the functional properties of the DCN network. This is mainly due to the difficulty of conducting in vitro experiments with this structure and the sparseness of intra-DCN-connections compared with the cortico-nuclear connections [2].

Phase response curves (PRCs) of regularly firing neurons quantify the shift in spike timing in response to perturbations [3]. The PRC is a strong theoretical tool allowing deduction of the behavior of a single neuron within a network [4]. Notably, the PRC can give insights to the emergent behavior of the neuron without detailed information about the ion channel composition. Here we propose a new bottom-up approach in forming hypotheses about the network properties in DCN, based on examining the phase response curves of individual neuronal types by 1) investigating the role of PRCs in discriminating different DCN cell types 2) formulating a hypothesis about the roles of these cells within the network, as different PRCs suggest involvement in different functional circuits. Since DCN neurons are spontaneously regularly firing, knowledge of the PRCs could allow significant conclusions about the in-vivo network behavior of the DCN.

We show that the large glutamatergic projection neurons of DCN exhibit type II PRCs and the local GABAergic neurons are characterized by type I PRC. In addtion, we present a single-compartmental model of DCN neuron dynamics.

References

  1. 1.
    Uusisaari M, Obata K, Knöpfel T: Morphological and electrophysiological properties of GABAergic and non-GABAergic cells in the deep cerebellar nuclei. J Neurophysiol. 2007, 97: 901-11. 10.1152/jn.00974.2006.CrossRefPubMedGoogle Scholar
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    Uusisaari M, Knöpfel T: GABAergic synaptic communication in the GABAergic and non-GABAergic cells in the deep cerebellar nuclei. Neuroscience. 2008, 156: 537-549. 10.1016/j.neuroscience.2008.07.060.CrossRefPubMedGoogle Scholar
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    Gutkin BS, Ermentrout GB, Reyes AD: Phase-Response Curves Give the Responses of Neurons to Transient Inputs. J Neurophysiol. 2005, 94: 1623-1635. 10.1152/jn.00359.2004.CrossRefPubMedGoogle Scholar
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    Netoff TI, Banks MI, Dorval AD, Acker CD, Haas JS, Kopell N, White JA: Synchronization in Hybrid Neuronal Networks of the Hippocampal Formation. J Neurophysiol. 2005, 93: 1197-1208. 10.1152/jn.00982.2004.CrossRefPubMedGoogle Scholar

Copyright information

© Uusisaari et al; licensee BioMed Central Ltd. 2010

This article is published under license to BioMed Central Ltd.

Authors and Affiliations

  • Marylka Uusisaari
    • 1
  • Benjamin Torben-Nielsen
    • 1
  • Klaus M Stiefel
    • 1
  1. 1.Theoretical and Experimental Neurobiology Unit, OISTOnna-sonJapan

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