Memili E, First NL: Zygotic and embryonic gene expression in cow: a review of timing and mechanisms of early gene expression as compared with other species. Zygote. 2000, 8 (1): 87-96. 10.1017/S0967199400000861.
CAS
PubMed
Google Scholar
Fair T, Carter F, Park S, Evans AC, Lonergan P: Global gene expression analysis during bovine oocyte in vitro maturation. Theriogenology. 2007, 68 (Suppl 1): S91-97. 10.1016/j.theriogenology.2007.04.018.
CAS
PubMed
Google Scholar
Hamatani T, Yamada M, Akutsu H, Kuji N, Mochimaru Y, Takano M, Toyoda M, Miyado K, Umezawa A, Yoshimura Y: What can we learn from gene expression profiling of mouse oocytes?. Reproduction. 2008, 135 (5): 581-592. 10.1530/REP-07-0430.
CAS
PubMed
Google Scholar
Fair T, Hyttel P, Greve T, Boland M: Nucleus structure and transcriptional activity in relation to oocyte diameter in cattle. Mol Reprod Dev. 1996, 43 (4): 503-512. 10.1002/(SICI)1098-2795(199604)43:4<503::AID-MRD13>3.0.CO;2-#.
CAS
PubMed
Google Scholar
Fair T, Hyttel P, Greve T: Bovine oocyte diameter in relation to maturational competence and transcriptional activity. Mol Reprod Dev. 1995, 42 (4): 437-442. 10.1002/mrd.1080420410.
CAS
PubMed
Google Scholar
Fenton SE, Dentine MR, Ax RL: Modulation of bovine oocyte-cumulus cell complex maturation and fertilization in vitro by glycosaminoglycans. J Dairy Sci. 1993, 76 (3): 701-712. 10.3168/jds.S0022-0302(93)77393-2.
CAS
PubMed
Google Scholar
Bevers MM, Izadyar F: Role of growth hormone and growth hormone receptor in oocyte maturation. Mol Cell Endocrinol. 2002, 197 (1-2): 173-178. 10.1016/S0303-7207(02)00270-8.
CAS
PubMed
Google Scholar
Sun FZ, Moor RM: Nuclear-cytoplasmic interactions during ovine oocyte maturation. Development. 1991, 111 (1): 171-180.
CAS
PubMed
Google Scholar
Heikinheimo O, Gibbons WE: The molecular mechanisms of oocyte maturation and early embryonic development are unveiling new insights into reproductive medicine. Mol Hum Reprod. 1998, 4 (8): 745-756. 10.1093/molehr/4.8.745.
CAS
PubMed
Google Scholar
Eichenlaub-Ritter U, Peschke M: Expression in in-vivo and in-vitro growing and maturing oocytes: focus on regulation of expression at the translational level. Hum Reprod Update. 2002, 8 (1): 21-41. 10.1093/humupd/8.1.21.
CAS
PubMed
Google Scholar
Moor RM, Crosby IM: Protein requirements for germinal vesicle breakdown in ovine oocytes. J Embryol Exp Morphol. 1986, 94: 207-220.
CAS
PubMed
Google Scholar
Hunter AG, Moor RM: Stage-dependent effects of inhibiting ribonucleic acids and protein synthesis on meiotic maturation of bovine oocytes in vitro. J Dairy Sci. 1987, 70 (8): 1646-1651. 10.3168/jds.S0022-0302(87)80192-3.
CAS
PubMed
Google Scholar
Lonergan P, Monaghan P, Rizos D, Boland MP, Gordon I: Effect of follicle size on bovine oocyte quality and developmental competence following maturation, fertilization, and culture in vitro. Mol Reprod Dev. 1994, 37 (1): 48-53. 10.1002/mrd.1080370107.
CAS
PubMed
Google Scholar
Lequarre AS, Vigneron C, Ribaucour F, Holm P, Donnay I, Dalbies-Tran R, Callesen H, Mermillod P: Influence of antral follicle size on oocyte characteristics and embryo development in the bovine. Theriogenology. 2005, 63 (3): 841-859. 10.1016/j.theriogenology.2004.05.015.
PubMed
Google Scholar
van de Leemput EE, Vos PL, Zeinstra EC, Bevers MM, van der Weijden GC, Dieleman SJ: Improved in vitro embryo development using in vivo matured oocytes from heifers superovulated with a controlled preovulatory LH surge. Theriogenology. 1999, 52 (2): 335-349. 10.1016/S0093-691X(99)00133-8.
CAS
PubMed
Google Scholar
Leroy JL, Vanholder T, Mateusen B, Christophe A, Opsomer G, de Kruif A, Genicot G, Van Soom A: Non-esterified fatty acids in follicular fluid of dairy cows and their effect on developmental capacity of bovine oocytes in vitro. Reproduction. 2005, 130 (4): 485-495. 10.1530/rep.1.00735.
CAS
PubMed
Google Scholar
Austin EJ, Mihm M, Ryan MP, Williams DH, Roche JF: Effect of duration of dominance of the ovulatory follicle on onset of estrus and fertility in heifers. J Anim Sci. 1999, 77 (8): 2219-2226.
CAS
PubMed
Google Scholar
Katz-Jaffe MG, McCallie BR, Preis KA, Filipovits J, Gardner DK: Transcriptome analysis of in vivo and in vitro matured bovine MII oocytes. Theriogenology. 2009, 71 (6): 939-946. 10.1016/j.theriogenology.2008.10.024.
CAS
PubMed
Google Scholar
Kues WA, Sudheer S, Herrmann D, Carnwath JW, Havlicek V, Besenfelder U, Lehrach H, Adjaye J, Niemann H: Genome-wide expression profiling reveals distinct clusters of transcriptional regulation during bovine preimplantation development in vivo. Proc Natl Acad Sci USA. 2008, 105 (50): 19768-19773. 10.1073/pnas.0805616105.
CAS
PubMed
PubMed Central
Google Scholar
Adjaye J, Herwig R, Brink TC, Herrmann D, Greber B, Sudheer S, Groth D, Carnwath JW, Lehrach H, Niemann H: Conserved molecular portraits of bovine and human blastocysts as a consequence of the transition from maternal to embryonic control of gene expression. Physiol Genomics. 2007, 31 (2): 315-327. 10.1152/physiolgenomics.00041.2007.
CAS
PubMed
Google Scholar
Jones GM, Cram DS, Song B, Magli MC, Gianaroli L, Lacham-Kaplan O, Findlay JK, Jenkin G, Trounson AO: Gene expression profiling of human oocytes following in vivo or in vitro maturation. Hum Reprod. 2008, 23 (5): 1138-1144. 10.1093/humrep/den085.
CAS
PubMed
Google Scholar
Lee YS, Latham KE, Vandevoort CA: Effects of in vitro maturation on gene expression in rhesus monkey oocytes. Physiol Genomics. 2008, 35 (2): 145-158. 10.1152/physiolgenomics.90281.2008.
CAS
PubMed
PubMed Central
Google Scholar
Lonergan P, Fair T: In vitro-produced bovine embryos: dealing with the warts. Theriogenology. 2008, 69 (1): 17-22. 10.1016/j.theriogenology.2007.09.007.
CAS
PubMed
Google Scholar
Trounson A, Anderiesz C, Jones G: Maturation of human oocytes in vitro and their developmental competence. Reproduction. 2001, 121 (1): 51-75. 10.1530/rep.0.1210051.
CAS
PubMed
Google Scholar
Watson AJ: Oocyte cytoplasmic maturation: a key mediator of oocyte and embryo developmental competence. J Anim Sci. 2007, 85 (13 Suppl): E1-3. 10.2527/jas.2006-432.
CAS
PubMed
Google Scholar
Rizos D, Ward F, Duffy P, Boland MP, Lonergan P: Consequences of bovine oocyte maturation, fertilization or early embryo development in vitro versus in vivo: implications for blastocyst yield and blastocyst quality. Mol Reprod Dev. 2002, 61 (2): 234-248. 10.1002/mrd.1153.
CAS
PubMed
Google Scholar
Sirard MA, Richard F, Blondin P, Robert C: Contribution of the oocyte to embryo quality. Theriogenology. 2006, 65 (1): 126-136. 10.1016/j.theriogenology.2005.09.020.
PubMed
Google Scholar
Krisher RL: The effect of oocyte quality on development. J Anim Sci. 2004, 82 (E-Suppl): E14-23.
PubMed
Google Scholar
Rijnders PM, Jansen CA: The predictive value of day 3 embryo morphology regarding blastocyst formation, pregnancy and implantation rate after day 5 transfer following in-vitro fertilization or intracytoplasmic sperm injection. Hum Reprod. 1998, 13 (10): 2869-2873.
CAS
PubMed
Google Scholar
Alper MM, Brinsden P, Fischer R, Wikland M: To blastocyst or not to blastocyst? That is the question. Hum Reprod. 2001, 16 (4): 617-619. 10.1093/humrep/16.4.617.
CAS
PubMed
Google Scholar
Papanikolaou EG, D'Haeseleer E, Verheyen G, Van de Velde H, Camus M, Van Steirteghem A, Devroey P, Tournaye H: Live birth rate is significantly higher after blastocyst transfer than after cleavage-stage embryo transfer when at least four embryos are available on day 3 of embryo culture. A randomized prospective study. Hum Reprod. 2005, 20 (11): 3198-3203. 10.1093/humrep/dei217.
PubMed
Google Scholar
Assou S, Anahory T, Pantesco V, Le Carrour T, Pellestor F, Klein B, Reyftmann L, Dechaud H, De Vos J, Hamamah S: The human cumulus--oocyte complex gene-expression profile. Hum Reprod. 2006, 21 (7): 1705-1719. 10.1093/humrep/del065.
CAS
PubMed
Google Scholar
Huarte J, Belin D, Vassalli A, Strickland S, Vassalli JD: Meiotic maturation of mouse oocytes triggers the translation and polyadenylation of dormant tissue-type plasminogen activator mRNA. Genes Dev. 1987, 1 (10): 1201-1211. 10.1101/gad.1.10.1201.
CAS
PubMed
Google Scholar
Pique M, Lopez JM, Mendez R: Cytoplasmic mRNA polyadenylation and translation assays. Methods Mol Biol. 2006, 322: 183-198. full_text.
CAS
PubMed
Google Scholar
Crozet N: Nulceolar fine structure and RNA synthesis in bovine oocytes from antral follicles. Gamete Research. 1986, 14: 65-73. 10.1002/mrd.1120140108.
CAS
Google Scholar
Cui XS, Li XY, Yin XJ, Kong IK, Kang JJ, Kim NH: Maternal gene transcription in mouse oocytes: genes implicated in oocyte maturation and fertilization. J Reprod Dev. 2007, 53 (2): 405-418. 10.1262/jrd.18113.
CAS
PubMed
Google Scholar
Thelie A, Papillier P, Perreau C, Uzbekova S, Hennequet-Antier C, Dalbies-Tran R: Regulation of bovine oocyte-specific transcripts during in vitro oocyte maturation and after maternal-embryonic transition analyzed using a transcriptomic approach. Mol Reprod Dev. 2009, 76 (8): 773-782. 10.1002/mrd.21031.
CAS
PubMed
Google Scholar
Su YQ, Sugiura K, Woo Y, Wigglesworth K, Kamdar S, Affourtit J, Eppig JJ: Selective degradation of transcripts during meiotic maturation of mouse oocytes. Dev Biol. 2007, 302 (1): 104-117. 10.1016/j.ydbio.2006.09.008.
CAS
PubMed
Google Scholar
Elsik CG, Tellam RL, Worley KC, Gibbs RA, Muzny DM, Weinstock GM, Adelson DL, Eichler EE, Elnitski L, Guigo R, et al: The genome sequence of taurine cattle: a window to ruminant biology and evolution. Science. 2009, 324 (5926): 522-528. 10.1126/science.1169588.
PubMed
PubMed Central
Google Scholar
Downs SM, Eppig JJ: Induction of mouse oocyte maturation in vivo by perturbants of purine metabolism. Biol Reprod. 1987, 36 (2): 431-437. 10.1095/biolreprod36.2.431.
CAS
PubMed
Google Scholar
Meinecke B, Meinecke-Tillmann S: Effects of alpha-amanitin on nuclear maturation of porcine oocytes in vitro. J Reprod Fertil. 1993, 98 (1): 195-201. 10.1530/jrf.0.0980195.
CAS
PubMed
Google Scholar
Memili E, First NL: Developmental changes in RNA polymerase II in bovine oocytes, early embryos, and effect of alpha-amanitin on embryo development. Mol Reprod Dev. 1998, 51 (4): 381-389. 10.1002/(SICI)1098-2795(199812)51:4<381::AID-MRD4>3.0.CO;2-G.
CAS
PubMed
Google Scholar
Sriram G, Martinez JA, McCabe ER, Liao JC, Dipple KM: Single-gene disorders: what role could moonlighting enzymes play?. Am J Hum Genet. 2005, 76 (6): 911-924. 10.1086/430799.
CAS
PubMed
PubMed Central
Google Scholar
Eppig JJ: Oocyte control of ovarian follicular development and function in mammals. Reproduction. 2001, 122 (6): 829-838. 10.1530/rep.0.1220829.
CAS
PubMed
Google Scholar
Gilchrist RB, Ritter LJ, Armstrong DT: Oocyte-somatic cell interactions during follicle development in mammals. Anim Reprod Sci. 2004, 82-83: 431-446. 10.1016/j.anireprosci.2004.05.017.
CAS
PubMed
Google Scholar
Gilchrist RB, Thompson JG: Oocyte maturation: emerging concepts and technologies to improve developmental potential in vitro. Theriogenology. 2007, 67 (1): 6-15. 10.1016/j.theriogenology.2006.09.027.
PubMed
Google Scholar
Dekel N: Cellular, biochemical and molecular mechanisms regulating oocyte maturation. Mol Cell Endocrinol. 2005, 234 (1-2): 19-25. 10.1016/j.mce.2004.09.010.
CAS
PubMed
Google Scholar
Tatemoto H, Terada T: Time-dependent effects of cycloheximide and alpha-amanitin on meiotic resumption and progression in bovine follicular oocytes. Theriogenology. 1995, 43 (6): 1107-1113. 10.1016/0093-691X(95)00074-I.
CAS
PubMed
Google Scholar
Schmitt A, Nebreda AR: Signalling pathways in oocyte meiotic maturation. J Cell Sci. 2002, 115 (Pt 12): 2457-2459.
CAS
PubMed
Google Scholar
Chen J, Chi MM, Moley KH, Downs SM: cAMP pulsing of denuded mouse oocytes increases meiotic resumption via activation of AMP-activated protein kinase. Reproduction. 2009, 138 (5): 759-770. 10.1530/REP-08-0535.
CAS
PubMed
PubMed Central
Google Scholar
Yoshimura Y, Nakamura Y, Oda T, Ando M, Ubukata Y, Karube M, Koyama N, Yamada H: Induction of meiotic maturation of follicle-enclosed oocytes of rabbits by a transient increase followed by an abrupt decrease in cyclic AMP concentration. J Reprod Fertil. 1992, 95 (3): 803-812. 10.1530/jrf.0.0950803.
CAS
PubMed
Google Scholar
Sanbuissho A, Coskun S, Lin YC: Role of cyclic adenosine monophosphate (cAMP) in vitro on bovine oocyte maturation. Theriogenology. 1992, 38 (1): 153-163. 10.1016/0093-691X(92)90227-I.
CAS
PubMed
Google Scholar
Guixue Z, Luciano AM, Coenen K, Gandolfi F, Sirard MA: The influence of cAMP before or during bovine oocyte maturation on embryonic developmental competence. Theriogenology. 2001, 55 (8): 1733-1743. 10.1016/S0093-691X(01)00516-7.
CAS
PubMed
Google Scholar
Lequarre AS, Traverso JM, Marchandise J, Donnay I: Poly(A) RNA is reduced by half during bovine oocyte maturation but increases when meiotic arrest is maintained with CDK inhibitors. Biol Reprod. 2004, 71 (2): 425-431. 10.1095/biolreprod.103.026724.
CAS
PubMed
Google Scholar
Leyens G, Verhaeghe B, Landtmeters M, Marchandise J, Knoops B, Donnay I: Peroxiredoxin 6 is upregulated in bovine oocytes and cumulus cells during in vitro maturation: role of intercellular communication. Biol Reprod. 2004, 71 (5): 1646-1651. 10.1095/biolreprod.104.030155.
CAS
PubMed
Google Scholar
Pocar P, Augustin R, Fischer B: Constitutive expression of CYP1A1 in bovine cumulus oocyte-complexes in vitro: mechanisms and biological implications. Endocrinology. 2004, 145 (4): 1594-1601. 10.1210/en.2003-1254.
CAS
PubMed
Google Scholar
Kastrop PM, Hulshof SC, Bevers MM, Destree OH, Kruip TA: The effects of alpha-amanitin and cycloheximide on nuclear progression, protein synthesis, and phosphorylation during bovine oocyte maturation in vitro. Mol Reprod Dev. 1991, 28 (3): 249-254. 10.1002/mrd.1080280306.
CAS
PubMed
Google Scholar
Stangegaard M, Dufva IH, Dufva M: Reverse transcription using random pentadecamer primers increases yield and quality of resulting cDNA. Biotechniques. 2006, 40 (5): 649-657. 10.2144/000112153.
CAS
PubMed
Google Scholar
Resuehr D, Spiess AN: A real-time polymerase chain reaction-based evaluation of cDNA synthesis priming methods. Anal Biochem. 2003, 322 (2): 287-291. 10.1016/j.ab.2003.07.017.
CAS
PubMed
Google Scholar
Ross DM, Watkins DB, Hughes TP, Branford S: Reverse transcription with random pentadecamer primers improves the detection limit of a quantitative PCR assay for BCR-ABL transcripts in chronic myeloid leukemia: implications for defining sensitivity in minimal residual disease. Clin Chem. 2008, 54 (9): 1568-1571. 10.1373/clinchem.2008.105916.
CAS
PubMed
Google Scholar
Zhang J, Byrne CD: Differential priming of RNA templates during cDNA synthesis markedly affects both accuracy and reproducibility of quantitative competitive reverse-transcriptase PCR. Biochem J. 1999, 337 (Pt 2): 231-241. 10.1042/0264-6021:3370231.
CAS
PubMed
PubMed Central
Google Scholar
Nam DK, Lee S, Zhou G, Cao X, Wang C, Clark T, Chen J, Rowley JD, Wang SM: Oligo(dT) primer generates a high frequency of truncated cDNAs through internal poly(A) priming during reverse transcription. Proc Natl Acad Sci USA. 2002, 99 (9): 6152-6156. 10.1073/pnas.092140899.
CAS
PubMed
PubMed Central
Google Scholar
Hyttel P, Greve T, Callesen H: Ultrastructural aspects of oocyte maturation and fertilization in cattle. J Reprod Fertil Suppl. 1989, 38: 35-47.
CAS
PubMed
Google Scholar
Sirard MA, Florman HM, Leibfried-Rutledge ML, Barnes FL, Sims ML, First NL: Timing of nuclear progression and protein synthesis necessary for meiotic maturation of bovine oocytes. Biol Reprod. 1989, 40 (6): 1257-1263. 10.1095/biolreprod40.6.1257.
CAS
PubMed
Google Scholar
Lonergan P, Khatir H, Carolan C, Mermillod P: Bovine blastocyst production in vitro after inhibition of oocyte meiotic resumption for 24 h. J Reprod Fertil. 1997, 109 (2): 355-365. 10.1530/jrf.0.1090355.
CAS
PubMed
Google Scholar
De Loos FA, Zeinstra E, Bevers MM: Follicular wall maintains meiotic arrest in bovine oocytes cultured in vitro. Mol Reprod Dev. 1994, 39 (2): 162-165. 10.1002/mrd.1080390207.
CAS
PubMed
Google Scholar
Belloc E, Pique M, Mendez R: Sequential waves of polyadenylation and deadenylation define a translation circuit that drives meiotic progression. Biochem Soc Trans. 2008, 36 (Pt 4): 665-670. 10.1042/BST0360665.
CAS
PubMed
Google Scholar
Lim LH, Pervaiz S: Annexin 1: the new face of an old molecule. FASEB J. 2007, 21 (4): 968-975. 10.1096/fj.06-7464rev.
CAS
PubMed
Google Scholar
Paciga M, DiMattia GE, Wagner GF: Regulation of luteal cell big stanniocalcin production and secretion. Endocrinology. 2004, 145 (9): 4204-4212. 10.1210/en.2003-1613.
CAS
PubMed
Google Scholar
Varghese R, Wong CK, Deol H, Wagner GF, DiMattia GE: Comparative analysis of mammalian stanniocalcin genes. Endocrinology. 1998, 139 (11): 4714-4725. 10.1210/en.139.11.4714.
CAS
PubMed
Google Scholar
Kao WW, Liu CY: Roles of lumican and keratocan on corneal transparency. Glycoconj J. 2002, 19 (4-5): 275-285. 10.1023/A:1025396316169.
CAS
PubMed
Google Scholar
Kao WW, Funderburgh JL, Xia Y, Liu CY, Conrad GW: Focus on molecules: lumican. Exp Eye Res. 2006, 82 (1): 3-4. 10.1016/j.exer.2005.08.012.
CAS
PubMed
Google Scholar
Ying S, Shiraishi A, Kao CW, Converse RL, Funderburgh JL, Swiergiel J, Roth MR, Conrad GW, Kao WW: Characterization and expression of the mouse lumican gene. J Biol Chem. 1997, 272 (48): 30306-30313. 10.1074/jbc.272.48.30306.
CAS
PubMed
Google Scholar
Lonergan P, Gutierrez-Adan A, Rizos D, Pintado B, de la Fuente J, Boland MP: Relative messenger RNA abundance in bovine oocytes collected in vitro or in vivo before and 20 hr after the preovulatory luteinizing hormone surge. Mol Reprod Dev. 2003, 66 (3): 297-305. 10.1002/mrd.10357.
CAS
PubMed
Google Scholar
Humblot P, Holm P, Lonergan P, Wrenzycki C, Lequarre AS, Joly CG, Herrmann D, Lopes A, Rizos D, Niemann H, et al: Effect of stage of follicular growth during superovulation on developmental competence of bovine oocytes. Theriogenology. 2005, 63 (4): 1149-1166. 10.1016/j.theriogenology.2004.06.002.
CAS
PubMed
Google Scholar
Bordignon V, Morin N, Durocher J, Bousquet D, Smith LC: GnRH improves the recovery rate and the in vitro developmental competence of oocytes obtained by transvaginal follicular aspiration from superstimulated heifers. Theriogenology. 1997, 48 (2): 291-298. 10.1016/S0093-691X(97)84076-9.
CAS
PubMed
Google Scholar
Gentleman RC, Carey VJ, Bates DM, Bolstad B, Dettling M, Dudoit S, Ellis B, Gautier L, Ge Y, Gentry J, et al: Bioconductor: open software development for computational biology and bioinformatics. Genome Biol. 2004, 5 (10): R80-10.1186/gb-2004-5-10-r80.
PubMed
PubMed Central
Google Scholar
Hochreiter S, Clevert DA, Obermayer K: A new summarization method for Affymetrix probe level data. Bioinformatics. 2006, 22 (8): 943-949. 10.1093/bioinformatics/btl033.
CAS
PubMed
Google Scholar
Talloen W, Clevert DA, Hochreiter S, Amaratunga D, Bijnens L, Kass S, Gohlmann HW: I/NI-calls for the exclusion of non-informative genes: a highly effective filtering tool for microarray data. Bioinformatics. 2007, 23 (21): 2897-2902. 10.1093/bioinformatics/btm478.
CAS
PubMed
Google Scholar
Tusher VG, Tibshirani R, Chu G: Significance analysis of microarrays applied to the ionizing radiation response. Proc Natl Acad Sci USA. 2001, 98 (9): 5116-5121. 10.1073/pnas.091062498.
CAS
PubMed
PubMed Central
Google Scholar
Ashburner M, Ball CA, Blake JA, Botstein D, Butler H, Cherry JM, Davis AP, Dolinski K, Dwight SS, Eppig JT, et al: Gene ontology: tool for the unification of biology. The Gene Ontology Consortium. Nat Genet. 2000, 25 (1): 25-29. 10.1038/75556.
CAS
PubMed
PubMed Central
Google Scholar
Huang da W, Sherman BT, Lempicki RA: Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat Protoc. 2009, 4 (1): 44-57. 10.1038/nprot.2008.211.
PubMed
Google Scholar
Dennis G, Sherman BT, Hosack DA, Yang J, Gao W, Lane HC, Lempicki RA: DAVID: Database for Annotation, Visualization, and Integrated Discovery. Genome Biol. 2003, 4 (5): P3-10.1186/gb-2003-4-5-p3.
PubMed
Google Scholar
Bower NI, Moser RJ, Hill JR, Lehnert SA: Universal reference method for real-time PCR gene expression analysis of preimplantation embryos. Biotechniques. 2007, 42 (2): 199-206. 10.2144/000112314.
CAS
PubMed
Google Scholar
Schmittgen TD, Livak KJ: Analyzing real-time PCR data by the comparative C(T) method. Nat Protoc. 2008, 3 (6): 1101-1108. 10.1038/nprot.2008.73.
CAS
PubMed
Google Scholar