NRG1 is required for glucose repression of the SUC2 and GAL genes of Saccharomyces cerevisiae
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Glucose repression of transcription in the yeast, Saccharomyces cerevisiae, has been shown to be controlled by several factors, including two repressors called Mig1 and Mig2. Past results suggest that other repressors may be involved in glucose repression.
By a screen for factors that control transcription of the glucose-repressible SUC2 gene of S. cerevisiae, the NRG1 gene was identified. Analysis of an nrg1Δ mutant has demonstrated that mRNA levels are elevated at both the SUC2 and of the GAL genes of S. cerevisiae when cells are grown under normally glucose-repressing conditions. In addition, genetic interactions have been detected between nrg1Δ and other factors that control SUC2 transcription.
The analysis of nrg1Δ demonstrates that Nrg1 plays a role in glucose repression of the SUC2 and GAL genes of S. cerevisiae. Thus, three repressors, Nrg1, Mig1, and Mig2, are involved as the downstream targets of the glucose signaling in S. cerevisiae.
KeywordsTriple Mutant Glucose Repression SUC2 Gene NRG1 Gene STA1 Gene
For the yeast Saccharomyces cerevisiae, glucose is the preferred carbon source. When glucose is present in the growth media, transcription of a large number of genes encoding products involved in the metabolism of alternative carbon sources is repressed (for reviews, see [1,2,3]. These genes include the GAL, SUC2, MAL and STA genes, required, respectively, for the utilization of galactose, sucrose/raffinose, maltose, and starch.
At many of these genes, glucose repression is mediated, at least in part, by the glucose-dependent repressor Mig1, a zinc-finger protein that binds in vitro to DNA consensus sites consisting of a GC-rich core and flanking AT sequences [4, 5]. Mig1 is thought to bind to several promoters, including GAL1, GAL4, SUC2 and MAL62, and to effect transcriptional repression by interacting with the co-repressor complex Ssn6-Tup1 [6,7,8]. Mig1's activity is regulated by phosphorylation and subcellular localization: in high glucose, Mig1 protein is hypophosphorylated and in the nucleus, where it can repress transcription; upon withdrawal of glucose, Mig1 is rapidly phosphorylated and transported into the cytoplasm . This regulated phosphorylation requires the function of the Snf1/Snf4 kinase complex .
Deletion of MIG1, however, only partially relieves glucose repression at promoters such as SUC2, whereas deletion of either SSN6 or TUP1 completely abolishes glucose repression. Moreover, the STA1 gene of S. cerevisiae var. diastaticus, which is also repressed by glucose, is unaffected by mig1Δ . Therefore, other proteins in addition to Mig1 are required for glucose repression. One of these proteins is Mig2, which shares sequence similarity with Mig1 in their zinc finger regions [12, 13]. Genetic analysis suggests that Mig2 plays a minor role relative to Mig1.
Recently, a previously uncharacterized gene, NRG1 (Negative regulator of glucose-repressed genes), was shown to be required for glucose repression of the STA1 gene in S. cerevisiae var. diastaticus . These studies demonstrated that LexA-Nrg1 behaves as a repressor of a reporter construct and that this repression is dependent on glucose, Ssn6, and Tup1. In addition, Nrg1 and Ssn6 interact with each other in two-hybrid and GST pull-down assays, indicating that Nrg1 may repress via the same pathway as Mig1. Consistent with these results, Nrg1 appears to bind to two sites within the STA1 promoter.
The SUC2 gene of S. cerevisiae has been extensively studied with respect to its glucose repression [1,2]. Glucose repression of SUC2 is mediated by Ssn6/Tup1 and SUC2 has two Mig1 binding sites in its regulatory region. Additionally, in high glucose its promoter is also occupied by positioned nucleosomes, which cause transcriptional repression themselves [14, 15]. Derepression in low glucose is correlated with a loss of both Mig1- and nucleosome-mediated repression, although the precise relationship between the two pathways is not clear.
Genetic screens have identified a large number of genes, named SNF (Sucrose Non-Fermenting) that are required for derepression of SUC2 transcription in the absence of glucose [16,17,18]. Genetic analyses and subsequent studies have traditionally divided SNF genes into two groups. One group encodes the protein kinase Snf1 and its associated regulator Snf4, required to antagonize the repression caused by Mig1 [10, 19]. The other group consists of members of the Swi/Snf complex required to counter the repressive effects of chromatin by remodeling nucleosomes in an ATP-dependent manner (for review see . Suppressors of swi/snf mutations, such as spt6, do not suppress snf1Δ , and ssn6, a strong suppressor of snf1Δ, only partially suppress swi/snf mutations .
In this work, we report the identification of Nrg1 in a genetic screen for new regulators of SUC2 transcription. We show that Nrg1 plays a role in the glucose repression of SUC2 and GAL genes in S. cerevisiae. Thus, at these genes, Mig1, Mig2 and Nrg1 are partially redundant for mediating repression by glucose. Consistent with our findings, recent results have demonstrated an interaction between Snf1 and Nrg1 . We also present experiments that test the genetic interactions between mig1Δ, nrg1Δ and deletions of various genes encoding activators that function at the SUC2 promoter.
Isolation of a high-copy-number suppressor of snf2Δ
The Swi/Snf complex is required for normal levels of expression of SUC2 when cells are grown in low glucose. To identify factors that might be functionally related to Swi/Snf, we screened for high-copy-number plasmids that could suppress a snf2Δ mutation (see Materials and Methods). To sensitize the screen, we used an allele of SUC2, SUC2-36, that allows an elevated level of SUC2 transcription in the absence of Swi/Snf . The SUC2-36 mutation is a single base pair change, AT to GC at position -401 relative to the SUC2 ATG. SUC2-36 strains still have a Raf- phenotype in a snf2Δ mutant.
MATα his3Δ 200 snf2Δ1::HIS3 ura3-52
MAT a his3Δ200 lys2-128δ snf2Δ1::HIS3 SUC2-36 ura3-52
MAT a /MATα his3Δ200/HIS3 LEU2/leu2Δ0 ura3Δ0/ura3Δ0
MAT a his3Δ200 leu2Δ0 ura3Δ0 nrg1Δ1::URA3
MATα his3Δ200 leu2Δ0 lys2Δ0 swp73Δ1::LEU2 ura3Δ0
MAT a leu2Δ0 snf1Δ10
MAT a his3Δ200 leu2Δ0 lys2Δ0 snf1Δ10 nrg1Δ1::URA3 ura3Δ0
MAT a his3Δ200 leu2Δ0 met15Δ0 snf2Δ2::LEU2 ura3Δ0
MAT a ade8 his3Δ200 leu2Δ0 met15Δ0 swi1Δ1::LEU2 ura3Δ0
MAT a his3Δ200 leu2Δ0 lys2Δ0 swp73Δ1::LEU2 nrg1Δ1::URA3 ura3Δ0
MAT a his3Δ200 leu2Δ0 snf2Δ2::LEU2 ura3Δ0 nrg1Δ1::URA3
MAT a his3Δ200 leu2Δ0 swilM::LEU2 nrg1Δ1::URA3 ura3Δ0
MATα his3Δ200 leu2Δ0 lys2-128δ ura3Δ0
MATα his3Δ200 leu2Δ0 lys2-128δ mig1-Δ2::LEU2 ura3Δ0
MAT a his3Δ200 leu2Δ0 lys2-128δ mig2Δ1::HIS3 ura3Δ0 nrg1Δ1::URA3
MAT a his3Δ200 leu2Δ0 lys2-128δ mig1-Δ2::LEU2 nrg1Δ1::URA3 ura3Δ0
MAT a his3Δ200 leu2Δ0 met15Δ0 mig1-Δ2::LEU2 mig2Δ1::HIS3 nrg1Δ1::URA3
MAT a his3Δ200 leu2Δ0 met15Δ0 mig1-Δ2::LEU2 mig2Δ1::HIS3 ura3Δ0
MAT a his3Δ200 leu2Δ0 met15Δ0 mig2Δ1::HIS3 ura3Δ0
MAT a his3Δ200 leu2Δ0 lys2-128δ mig1-Δ2::LEU2 ura3Δ0
MAT a his3Δ200 leu2Δ0 lys2-128δ mig1-Δ2::URA3 snf2Δ2::LEU2 ura3Δ0
MAT a his3Δ200 leu2Δ0 lys2-128δ mig1-Δ2::URA3 swi1Δ1::LEU2 ura3Δ0
MAT a his3Δ200 leu2Δ0 lys2-128δ mig1-Δ2::URA3 swp73Δ1::LEU2 ura3Δ0
MAT a his3Δ200 leu2Δ0 lys2-128δ mig1-Δ2::URA3 snf1Δ10 ura3Δ0
MATα his3Δ200 leu2Δ0 lys2-128δ swi1Δ1::LEU2
NRG1 is predicted to encode a protein of 231 amino acids with two C2H2 zinc fingers in the carboxyl terminus. Sequence analysis revealed that the 2μ plasmid that confers suppression of snf2Δ encodes just the amino terminal region of Nrg1, lacking the zinc fingers. To test if the complete NRG1 gene causes the same high copy number phenotype, we subcloned the complete NRG1 gene into a 2μ plasmid and tested it for suppression of snf2Δ. Our results demonstrate that the complete NRG1 gene on a 2μ plasmid does not suppress snf2Δ (Figure 1).
NRG1 encodes a repressor of transcription
To characterize further the role of Nrg1 with respect to SUC2 transcription, we constructed and analyzed an nrg1Δ mutant. The nrg1Δ mutant grows normally on media containing glucose, sucrose, or galactose, demonstrating that NRG1 is not essential for grwoth and that nrg1Δ mutants can utilize several different carbon sources.
Glucose repression of transcription is defective in nrg1Δ
We also tested if an nrg1Δ affects glucose repression of the GAL genes as described in Materials and Methods. Both nrg1Δ and mig1Δ mutations cause a defect in the glucose repression of GAL1 and GAL10, whereas mig2Δ alone had no effect (Figure 3B). As for SUC2, additive effects were observed in double and triple mutant strains, up to a 13-fold effect for the nrg1Δ mig1Δ mig2Δ triple mutant (Figure 3B). These data indicate that all three proteins are involved in glucose repression of GAL1-GAL10, with Mig2 playing only a minor role.
Deletion of MIG1 or NRG1 suppresses mutations in both SNF1 and SWI/SNF genes
Activation of SUC2 transcription depends upon both the Snf1/Snf4 kinase complex and the Swi/Snf nucleosome remodeling complex. To address the relationship of Nrg1 to both complexes and to compare it to Mig1, we tested the abilities of nrg1Δ and mig1Δ to suppress the Gal-, Suc-, and Raf- phenotypes of mutations in SNF1 and SWI/SNF genes.
Our results demonstrate that Nrg1 plays a role in glucose repression of the SUC2 and GAL genes of S. cerevisiae. Consistent with a role in glucose repression, an nrg1Δ mutation suppresses the defects of a snf1Δ mutant. Recent results from an independent study have demonstrated an interaction between Snf1 and Nrg1 . Our results also suggest that Nrg1 is partially redundant with two other factors required for glucose repression, Mig1 and Mig2. At SUC2 and GAL1-10, all three proteins appear to be involved in glucose repression, because double- and triple-deletion mutations have additive effects. Interestingly, both nrg1Δ and mig1Δ can also suppress the defects caused by mutations in genes encoding members of the Swi/Snf complex.
While Nrg1, Mig1, and Mig2 are partially redundant, current evidence suggestions that they do not function in the same relative fashion at all glucose-repressible promoters. For example, while mig1Δ and nrg1Δ cause comparable defects at GAL1-GAL10, nrg1Δ causes a weaker defect at SUC2. Mig2 appears to have only a minimal function at either promoter. In addition, Nrg1 is the major repressor at STA1, whose glucose-repression does not require Mig1 . Therefore, some gene-specific specialization exists among these three glucose-dependent repressors.
A previous study of Nrg1 provided evidence that it interacts with Ssn6 and confers repression by recruitment of Ssn6/Tup1 . We initially identified NRG1 in our studies by the isolation of a high-copy-number plasmid encoding a fragment of Nrg1, lacking the zinc-finger domain. Likely, the phenotype caused by this plasmid is caused by interference of repression by Ssn6/Tup1.
Our studies have not yet distinguished between a direct or indirect effect of Nrg1 on glucose repression at SUC2 and GAL1-GAL10. One possible indirect effect of Nrg1 could be by regulation of MIG1 transcription. However, Northern analysis showed that MIG1 mRNA levels are unaffected by an nrg1Δ mutation (H. Zhou and F. Winston, unpublished data). We tested Nrg1 for binding to the SUC2 promoter and those experiments are briefly summarized here. We screened for DNA binding of Nrg1 to the SUC2 promoter region using a previously described GST-Nrg1 fusion protein  and a gel shift assay. Our results demonstrated specific DNA binding to two sites within the -1022 to -825 region 5' of SUC2 (H. Zhou and F. Winston, unpublished results). However, a deletion of this region does not alter SUC2 expression. Based on the similarity between the zinc fingers of Nrg1 and Mig1 and our binding studies, the binding site of Nrg1 may contain a GC-rich core. Another such site in the SUC2 promoter may occur at -570 with the sequence AGGCCCA. Although we did not detect a gel shift of a fragment containing this site, it is still possible that it is recognized and bound by Nrg1 in vivo. Furthermore, although an Nrg1 consensus binding  exists at -976 of SUC2, we were unable to detect binding to this site by GST-Nrg1. This region also did not compete the binding that we detected by GST-Nrg1. This discrepancy between our findings and previous results can be explained by the fact that Park et al  used 10-fold more GST-Nrg1 in their binding studies than we did. Finally, we did not detect any binding of Nrg1 to the Mig1 binding sites. Thus, the DNA binding of Nrg1 to SUC2 remains to be resolved.
In conclusion, these studies have identified Nrg1 as a third repressor required for glucose repression at SUC2 and the GAL genes. Based on the similarity between the zinc fingers of Nrg1 and Mig1, the phenotypes of nrg1Δ and mig1Δ, and the reported interaction between Nrg1 and Ssn6 , Nrg1 likely functions by binding to the target promoters and recruiting the Ssn6/Tup1 complex. The relative and possible cooperative roles of each of these repressors in recruiting Ssn6-Tup1 remains to be determined.
Materials and methods
All S. cerevisiae strains are listed in Table 1 and are in the S288C genetic background [25, 26]. Deletion of MIG1 was achieved by transforming strain yHZ416 with the Hind III digest of pJN22 (for migl-Δ2::LEU2) or pJN41 (for mig1-Δ2::URA3) , and selecting for Leu+ or Ura+ transformants, respectively. PCR-directed gene replacement  was used to construct deletions of NRG1 and MIG2. PCR reactions were carried out using as templates pRS vectors carrying the desired markers [25, 28]. For NRG1, the oligos used were HZ034, 5' TCG ACC AGC ATA TTA CTA CCC TTC GCA AAC TTT CAG GCA CTG TGC GGT ATT TCA CAC CG 3'; and HZ035, 5' GTA GTA CTG CTA ATG AGA AAA ACA CGG GTA TAC CGT CAA AGA TTG TAC TGA GAG TGC AC 3'. For MIG2, the oligos were HZ045, 5' TGA CCT CGA GAA CAA ACA AAA TAA AAA TAA AAA AAG AGA CTG TGC GGT ATT TCA CAC CG 3'; and HZ046, 5' TTA GAG GAA AAA TGG TGA GAT AAA AAG GGG CCG TAA AGG AGA TTG TAC TGA GAG TGC AC 3'. The PCR fragment was used to transform a haploid strain directly. All gene replacements were verified by PCR, Southern analyses, and tetrad analyses.
The media used in this study were previously described . Glucose, galactose, sucrose or raffinose was added to 2% final weight per volume. For solid media containing a carbon source other than glucose or glycerol, antimycin A was also added to a concentration of 1 μg/ml. To test for glucose repression of SUC2 and GAL genes, 2-deoxyglucose was added to YP sucrose-antimycin A and YP galactose-antimycin A plates to a final concentration of 200 μg/ml . We discovered during the course of this study that a ura3Δ0 strain had half the amount of GAL 1-10 mRNA of a URA3 strain when grown in SD media containing 2% glucose and 2% galactose. A ura3Δ0 strain also grew more slowly than a URA3 strain on minimal media containing sucrose or galactose. We do not yet have an explanation for this phenomenon. To overcome this growth defect, uracil was added to YP plates to a final concentration of 80 μM.
Subcloning of NRG1 constructs
The 1.8 kb SacI-SalI fragment of the original library clone, containing only the 5' half of NrG1 without the zinc fingers, was cloned into the SacI-SalI sites of pRS426 to create pHZ56. To clone the complete NRG1 ORF, HZ032 and HZ033 were used to PCR from genomic DNA the complete wild-type NRG1 from -1119 to +719. The PCR fragment was digested with Sad and cloned into the SacI-SmaI sites of pRS426 to generate pHZ52.
Cell cultures were grown in liquid media as indicated to mid-log phase (1-2 × l07 cells/ml), and total RNA was prepared as previously described [27,30]. RNA was separated by electrophoresis on 1% agarose-formaldehyde gels, transferred to membrane and blotted with specific radio-labeled probes. The probes were: for SUC2, the 1.3 kb Bam HI-Hind III fragment of pRB59 ; for GAL1-10, the 2 kb Eco RI-Eco RI fragment of BNN45  and for SPT15, the 0.8 kb Spe I-Hind III fragment of pIP45 (I. Pinto, personal communication). All probes were labeled by random priming.
This work was supported by NIH grant GM32967.
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