Identification of a novel Rev-interacting cellular protein
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Human cell types respond differently to infection by human immunodeficiency virus (HIV). Defining specific interactions between host cells and viral proteins is essential in understanding how viruses exploit cellular functions and the innate strategies underlying cellular control of HIV replication. The HIV Rev protein is a post-transcriptional inducer of HIV gene expression and an important target for interaction with cellular proteins. Identification of Rev-modulating cellular factors may eventually contribute to the design of novel antiviral therapies.
Yeast-two hybrid screening of a T-cell cDNA library with Rev as bait led to isolation of a novel human cDNA product (16.4.1). 16.4.1-containing fusion proteins showed predominant cytoplasmic localization, which was dependent on CRM1-mediated export from the nucleus. Nuclear export activity of 16.4.1 was mapped to a 60 amino acid region and a novel transport signal identified. Interaction of 16.4.1 with Rev in human cells was shown in a mammalian two-hybrid assay and by colocalization of Rev and 16.4.1 in nucleoli, indicating that Rev can recruit 16.4.1 to the nucleus/nucleoli. Rev-dependent reporter expression was inhibited by overexpressing 16.4.1 and stimulated by siRNAs targeted to 16.4.1 sequences, demonstrating that 16.4.1 expression influences the transactivation function of Rev.
These results suggest that 16.4.1 may act as a modulator of Rev activity. The experimental strategies outlined in this study are applicable to the identification and biological characterization of further novel Rev-interacting cellular factors.
KeywordsHuman Immunodeficiency Virus Nuclear Export Cytoplasmic Localization Nuclear Export Signal Amino Acid Region
The human immunodeficiency virus (HIV) Rev protein is a small (116 amino acids) post-transcriptional activator of expression of incompletely spliced and unspliced HIV mRNAs. Since these HIV transcripts direct production of progeny virions, Rev is a crucial factor in HIV replication (for overview see ). Rev interacts with HIV mRNAs by binding to a structured RNA element called the RRE (Rev response element). Rev offsets the activities of inhibitory sequences (INS) in HIV-1 mRNAs [2, 3] and promotes their export to the cytoplasm. Once in the cytoplasm, Rev may also stimulate production of viral proteins on translational level (reviewed in ).
Rev characteristically localizes to the nucleus, where it accumulates in nucleoli. However, a proportion of the Rev molecules expressed in a cell continuously shuttles between nucleus and cytoplasm by using active transport mechanisms both for entry into and exit from the nucleus.
Biochemical analyses indicate that Rev directly binds the nuclear transport receptors Importin β and CRM1/Exportin 1 [7, 8, 9]. Interaction of Rev with CRM1/Exportin 1 was confirmed by two-hybrid assays in yeast  and in human cells [11, 12]. Together with results from various other experimental approaches (reviewed in ), these observations have led to the concept that import of Rev into the nucleus is mediated by interaction of the ARM/NLS with Importin Open image in new window and export of the Rev-RNA complex from the nucleus by interaction of the Rev-NES with CRM1/Exportin 1.
Various other Rev-interacting cellular factors have been identified by using Rev or segments of Rev for yeast two-hybrid screening of cDNA libraries or for biochemical purification of interacting factors from cell extracts.
Cellular factors shown to interact with the ARM of Rev include p32 [13, 14] and B23 [15, 16]. Human p32 was recently reported to block splicing of Rev-dependent HIV transcripts . The nucleolar protein B23 was shown to stimulate nuclear import of Rev  and counteract aggregation of Rev in vitro.
The C-terminal domain of Rev interacts with various human nucleoporins, including hRIP/hRab, NLP-1, Nup98, and Nup214 [20, 21, 22, 23, 24, 25, 26]. Other factors shown to interact with this domain of Rev are eIF-5A  and the nuclear kinesin-like protein REBP . hRIP/hRab, Nup 98 and eIF-5A interact with CRM1 as well as Rev [10, 25, 26, 29], suggesting that Rev can associate with CRM1 in multifactorial complexes in which CRM1 "bridges" the interaction of Rev with other factors. Rev-CRM1 complexes containing hRIP/hRab or eIF-5A may be crucial for Rev-dependent export of HIV RNAs, since eIF-5A and hRIP/hRab have been shown to be essential for Rev-directed RNA export in Xenopus oocytes and in human cells, respectively [30, 31].
Nuclear export of Rev has proven to be exemplary for many viral and cellular factors (reviewed in [32, 33]). Since the discovery of leucine-rich signals in Rev  and in the cellular regulatory factor PKIα , these sequences have been shown to mediate the export of numerous factors from the nucleus by CRM1/Exportin1 . The drug Leptomycin B (LMB), first shown to block nuclear export of Rev , proved to be a potent inhibitor of CRM1-dependent export [38, 39] and is now widely used to identify transport substrates of CRM1. Elucidating interactions of Rev with cellular factors is highly relevant to understanding pathogenicity of HIV and may have an impact on the design of therapeutic anti-HIV strategies. The functional diversity of Rev and its activities in both nuclear and cytoplasmic compartments of the cell suggest the existence of still unidentified Rev-interacting factors. Therefore we reasoned that screening of a human cDNA library with Rev as "bait" should lead to isolation of novel Rev-interacting human factors. Of particular interest would be the identification of unknown human gene products, since their interaction Rev would not only be relevant for Rev function but would also provide a key for biological characterisation of these novel factors.
Here we identify a human cDNA that encodes a novel protein (16.4.1) that interacts specifically with Rev via sequences in the N-terminal half of Rev. We show that 16.4.1 is exported from the nucleus by CRM1 and localizes to the cytoplasm. In Rev-expressing cells, 16.4.1 is recruited to nucleoli. 16.4.1 has a negative effect on Rev function in a Rev-reporter assay. These results suggest that 16.4.1 can act as a modulator of Rev function.
Identification of novel HIV-1 Rev-interacting proteins
To identify novel Rev-interacting proteins, we screened a library of cDNAs derived from the human Jurkat T-cell line with full-length Rev as bait in a yeast two-hybrid system. Repeated selection procedures led to isolation of two library plasmids (11.5.1 and 16.4.1) encoding specific interactors of Rev.
Sequence analyses and data base comparisons revealed that the 936 bp insert in plasmid 11.5.1 is identical with a segment of a 1543 bp cDNA encoding human DNA binding protein B (dbpB; NCBI accession number BC002411) . The predicted coding sequences in the 11.5.1 cDNA comprise the C-terminal 139 amino acids of the dbpB protein (324 amino acids; NCBI accession number M24070). Several biological activities have been attributed to dbpB, including binding to DNA  and RNA [42, 43] and regulation of transcription .
The other library plasmid 16.4.1 contained a 696 bp insert of which a region of over 450 nucleotides showed strong similarity to a sequence within a human fetal heart cDNA (NCBI accession number W67699). In the fetal heart cDNA the matching region encompasses a predicted open reading frame. Alignment of the 16.4.1 and the fetal heart cDNA sequences yielded a sequence encoding a hypothetical 171 amino acid 16.4.1 protein. Since interaction with Rev is the first biological activity associated with this gene product, we analysed interaction of Rev with the 16.4.1 protein in more detail.
To investigate which regions of Rev contribute to interaction with the 16.4.1 protein, we analysed the capacity of various known mutants of Rev to interact with 16.4.1 in the yeast two-hybrid assay. The amino acid exchanges in these mutants map to regions associated with major biological properties of Rev (Fig. 1B), including multimerization (RevM4  and RevSLT40 ), RNA binding and nuclear localization/accumulation (RevM5 ) and nuclear export of Rev (RevM10BL ). Expression of LexA-Rev-mutant bait proteins in yeast transformants was confirmed by Western blot analysis with polyclonal antibodies against Rev (data not shown). As positive control for Rev interaction, interaction analysis was performed with LexA-Rev bait and B42AD-Rev prey, confirming oligomerization of wildtype Rev molecules with each other (data not shown).
While Rev mutants RevM4 and Rev M10BL were capable of interacting with 16.4.1, no interaction was observed with Rev mutants RevM5 and RevSLT40 (Fig. 1B). These results indicate that amino acid residues R38 or R39 of the ARM and I59 or L60 of the multimerization region II (MII) are required for interaction of Rev with the 16.4.1 protein. Furthermore, they suggest that the 16.4.1 interacting sequences in Rev are located between aa positions 38 and 60.
For more detailed study of the interaction of the 16.4.1 protein with Rev, yeast two-hybrid analysis was performed with various segments of the 16.4.1 cDNA as prey and wildtype Rev as bait (Fig. 1C). Amino acid regions of 16.4.1 extending from position 2 to 133 and from position 39 to 171 showed similar Rev-binding capacity as full-length 16.4.1 protein. In contrast, both the N-terminal region (2 to 38) and the C-terminal region (134 to 171) of 16.4.1 failed to interact with Rev. While 16.4.1 protein fragments from position 2 to 73 or position 74 to 171 clearly interacted with Rev, interactions were weaker than that of full-length 16.4.1. These results indicate that the Rev-interacting region of the 16.4.1 protein is located between amino acid positions 39 and 133 and that, within this region, sequences N- and C-terminal of position 73 contribute to interaction with Rev.
Interaction of the 16.4.1 protein with Rev, CRM1 and itself in human cells
The interaction of the 16.4.1 protein with Rev in yeast raises the question whether the 16.4.1 protein can also interact with Rev in human cells. It was also of interest whether 16.4.1 is capable of interacting with human CRM1, since CRM1 has been shown to interact with several Rev-associated factors (see Background).
In all three cases, induction of luciferase activity was significantly (p < 0.04) increased over induction levels obtained in control assays with unfused VP16 and Gal4-16.4.1 (3.3-fold; n = 7).
These results indicate that the 16.4.1 domain is capable of interacting with Rev as well as with the export receptor CRM1 and of forming homo-oligomers in human cells.
Cytoplasmic localization of 16.4.1 is CRM1/Exportin 1 dependent
The 16.4.1-GFP fusion protein showed similar cytoplasmic localization as IgG1-16.4.1 (Fig. 3B). Quantitative evaluation of subcellular distribution of GFP fluorescence  revealed that only 25% of total fluorescence was contained in the nuclei of 16.4.1-GFP expressing cells. This localization is comparable to that of GFP fusion proteins containing PKIα (PKIα-GFP) or the carboxyterminal half of Rev (Rev(52–116)-GFP), which localize to 23% and 25%, respectively, in the nucleus (Fig. 3B). PKIα and the carboxyterminal half of Rev contain well-characterized recognition signals for CRM1/Exportin 1-dependent export . Similar cytoplasmic localization of 16.4.1-GFP and interaction of 16.4.1 with CRM1/Exportin 1 in human cells (Fig. 2) raised the possibility that cytoplasmic localization of 16.4.1-GFP at steady state may involve nuclear export of 16.4.1 by CRM1/Exportin 1. Therefore we analysed the effect of Leptomycin B (LMB), an inhibitor of CRM1-dependent nuclear export [11, 37, 38] on subcellular distribution of 16.4.1-GFP. LMB treatment significantly increased the nuclear proportion of 16.4.1-GFP from 25% to 44%. LMB-induced nuclear redistribution was similar in cells expressing PKIα-GFP and Rev(52–116)-GFP, whose nuclear proportion increased to 49% and 46%, respectively. Quantitative analysis demonstrated that 45% of unfused GFP localized to the nucleus, in agreement with its known capacity to diffuse throughout the cell . LMB had no significant effect on subcellular distribution of unfused GFP.
These results indicate that cytoplasmic localization of 16.4.1 involves nuclear export by CRM1/Exportin1. Amino acid region 74 to 133 of 16.4.1 seems to be crucial for those transport processes.
Identification of a candidate nuclear export signal in 16.4.1
Finally we investigated whether the candidate transport signal also shows nuclear export activity in the context of the complete 16.4.1 protein. As shown in figure 6B, the leucine and two isoleucine residues of the 16.4.1 core NES were changed to Alanin and the subcellular distribution of the 16.4.1(NESmut)-GFP was compared to the wildtype 16.4.1 fused to GFP. The mutant 16.4.1-GFP fusion protein localized to significantly higher levels in the nucleus than wildtype 16.4.1-GFP (34% versus 27%). However, the nuclear proportion of the mutant 16.4.1-GFP remained below that of unfused GFP (Fig. 3B), indicating residual nuclear export of the mutant 16.4.1-GFP.
In summary, combined computational and functional analyses indicate that amino acid residues 86 to 105 act as a nuclear export signal, with amino acids 92 to 99 constituting a potential core NES. Mutational analysis indicates that the leucine/isoleucine of the 16.4.1 core NES contribute to but are not sole determinants of cytoplasmic localization of 16.4.1.
Colocalization of 16.4.1 and Rev
This report demonstrates interaction of 16.4.1 and Rev in yeast and mammalian two-hybrid assays (Figs. 1 and 2). In these approaches, candidate interaction partners are artificially targeted to the nucleus to measure interaction-dependent reporter gene expression.
To analyse whether 16.4.1 and Rev interact under conditions in which they retain their natural localization behavior, we analysed cells coexpressing 16.4.1 and Rev for colocalization of both proteins.
To this end, we first established a HeLa cell line stably expressing 16.4.1-GFP and a corresponding control cell line expressing unfused GFP. The expression of 16.4.1-GFP for more than 20 passages did not affect cell growth monitored by measurement of growth curves and did not lead to cell toxicity detectable as release of lactate dehydrogenase (LDH) or ATP into cell culture supernatants (data not shown). Furthermore, long-term expression did not alter the predominantly cytoplasmic localization of 16.4.1-GFP.
These results indicate that Rev is capable of directing 16.4.1 to nucleoli and provide further evidence for interaction of Rev and 16.4.1 in human cells.
Influence of 16.4.1 on Rev functions
The transactivation capacity of Rev in the absence of exogenous 16.4.1 was set at 100%. The result of 5 independent experiments demonstrate an approximately 50% reduction of Rev activity by coexpression of 16.4.1 fusion proteins (Fig. 8A). A dose-dependent effect of 16.4.1-GFP expression on Rev activity was observed (Fig. 8B). No effect was observed for numerous other gene products of a human cDNA-library tested in this assay (Wolff et al, manuscript in preparation).
Subsequently we investigated the effect of siRNA-16.4.1 and the negative control siRNA-nsp in 293T cells in the Rev-reporter assay described above (Fig. 9C). The negative control siRNA (siRNA-nsp) had no effect on Rev transactivation capacity compared to mock transfected cells (1% increase of Rev activity). In contrast, siRNA-16.4.1 increased Rev transactivation capacity by 17% compared to mock-transfected controls. A specific enhancing effect of siRNA-16.4.1 was observed in three independent experiments. These results indicate that endogenous 16.4.1 gene products are capable of modulating Rev activity.
Expression of 16.4.1 proteins
Database searches identified several cDNAs of various lengths that contain the complete 16.4.1 sequence within a predicted open reading frame [For overview see additional file 1: Figure A1 and additional file 2: Table A1]. These are derived from various human tissues and cells. The predicted molecular masses of the hypothetical proteins encoded by these cDNAs range from ~145 kDa to 18.5 kDa. This suggests existence of several human 16.4.1 protein species encoded by various cDNAs, rather than a single 16.4.1 protein generated from a single cDNA.
Production of 16.4.1 proteins has not been reported so far and is currently under investigation in our laboratory (Kramer-Hämmerle et al., in preparation). In the context of this ongoing study, a monoclonal antibody (Mab) was generated against recombinant 16.4.1. Indirect immunofluorescence of HeLa cells transfected with the IgG1-16.4.1 expression plasmid revealed a cytoplasmic staining pattern that was indistinguishable from that obtained with antibodies against IgG1 [see additional file 3: Figure A2, A) a, for image]. The 16.4.1 Mab, but not the secondary antibody, also stained untransfected HeLa cells, yielding a cytoplasmic, granular pattern [see additional file 3, Figure A2, A) b for image]. In Western Blot analysis of HeLa-16.4.1-GFP cells, the 16.4.1 Mab recognized a band with the predicted molecular mass of ~45 kDa for the 16.4.1-GFP fusion protein as well as additional proteins [see additional file 3, Figure A2, B]. These results confirm specific recognition of 16.4.1 antigens by the 16.4.1 Mab and indicate expression of endogenous 16.4.1 proteins.
The 16.4.1 Mab has also been used to analyze cells from different lines and primary tissues, including brain and peripheral blood mononuclear cells. A staining pattern similar to that in HeLa cells was observed for 4 out 5 cell lines analyzed by indirect immunofluorescence (not shown). Western blot pagesanalysis of the cell lines/tissues investigated so far yielded a total of 4 distinct bands, ranging in size from > 150 kDa to < 30 kDa [for summary see additional file 3, Table A2]. The occurrence of these bands depended on the cell line/tissue investigated. These results confirm expression of 16.4.1 proteins in human cells and tissues and suggest cell-specific expression patterns of 16.4.1 proteins.
Future studies are directed at identifying the full range of 16.4.1 protein species with a panel of antibodies and characterizing 16.4.1 expression patterns on cDNA and protein levels in various cell types.
In this study we identified a cDNA encoding a novel cellular gene product (16.4.1) that interacts with the HIV-1 Rev protein in yeast and mammalian cells. In human cells, 16.4.1 is a substrate for CRM1-dependent export and shows predominant cytoplasmic localization. Colocalization of Rev and 16.4.1 was observed in nuclei, particularly in the nucleoli, of cells expressing both proteins. Overexpression and RNAi experiments indicate that 16.4.1 can influence transactivation function of Rev.
Comparison of cytoplasmic localization properties of 16.4.1, Rev and PKI
We demonstrate that 16.4.1 interacts with CRM1 (Fig. 2) and shows similar Leptomycin B sensitive, cytoplasmic localization behaviour as PKIα and the carboxyterminal half of Rev (Fig. 3B) which are known substrates for CRM1-dependent export . These results indicate that cytoplasmic localization of 16.4.1 involves CRM1-mediated nuclear export.
A nuclear export signal was mapped in 16.4.1 (Fig 5). Mutation of the Leucine/Isoleucine residues of the 16.4.1 NES only partially inhibited nuclear export of full-length 16.4.1, whereas leucine/isoleucine residues in the NES have been shown to be essential for export of Rev and PKIα [35, 53]. This suggests differences in the export functions of the 16.4.1 NES and the NES of Rev and PKIα. This conclusion is supported further by the bioinformatics analysis, which showed that the group of NES sequences recognized by the same matrix as the 16.4.1 transport signal did not include the NES of Rev or PKIα (Fig. 6).
GFP fusion proteins containing a single copy of the 16.4.1 NES showed weaker cytoplasmic localization than GFP fusion proteins with tandem copies of this region or full-length 16.4.1-GFP (Fig. 4). This suggests that cytoplasmic localization of 16.4.1 does not depend solely on the functionality of a single copy of the 16.4.1 NES. The formation of homo-oligomers of 16.4.1, as shown by mammalian two-hybrid analysis (Fig. 2), could allow cooperative activity of multiple 16.4.1 NES. In addition, sequences beyond the NES could also contribute to cytoplasmic localization, for example by increasing cytoplasmic retention of 16.4.1. Sequences beyond the NES of 16.4.1 could also promote interactions with export-enhancing co-factors, several of which have been identified so far. These include the Ran-binding protein 3 (RanBP3) [54, 55], NXT1  and eukaryotic initiation factor 5A (eIF-5A). eIF-5A was demonstrated to be involved in export of Rev-like NES but not of the PKIα-NES [30, 57], suggesting the existence of substrate-specific export cofactors. Future studies will be directed at identifying cellular interaction partners of the 16.4.1 protein and investigating their influence on its export activity.
Interactions of 16.4.1 and Rev
In this study we show that 16.4.1 and Rev are capable of influencing biological properties of one another.
In cells expressing 16.4.1 and Rev, Rev can alter localization properties of 16.4.1 by recruiting 16.4.1 to the nucleus, in particular nucleoli. This is shown by colocalization of Rev and 16.4.1 in the nucleoli of cells expressing both proteins (Fig. 7). Cytoplasmic localization of 16.4.1 suggests that 16.4.1 interacts with Rev in the cytoplasm and is then translocated together with Rev to the nucleus and to nucleoli. The region of 16.4.1 that mediates interaction with Rev (amino acids 39–133, Fig. 1) contains the 16.4.1 NES (amino acids 86–105). Thus CRM1 could "bridge" interaction of 16.4.1 with Rev. CRM1-mediated interaction with Rev has been observed for several cellular proteins proposed to function as cofactors for nuclear export of Rev (see Background). However, amino acid positions of Rev essential for interaction with 16.4.1 are located outside the Rev NES, and an export-deficient NES-mutant of Rev (RevM10Bl) was capable of interacting with 16.4.1 (Fig. 1). This suggests that 16.4.1 does not function as an essential cofactor for nuclear export of Rev.
We have demonstrated that overexpression of 16.4.1 inhibits transactivation function of Rev (Fig. 8). The molecular mechanism underlying this inhibitory effect is unclear. A possible model to explain an inhibitory effect of 16.4.1 on Rev activity is that 16.4.1 recruited to nucleoli by Rev promotes association of Rev and CRM1 in inactive complexes. The strong interaction of 16.4.1 with CRM1 may increase the amount of CRM1 associated with Rev to inhibitory levels. In support of this model, experimental evidence has been obtained demonstrating that (1) Rev associates with CRM1 in nucleoli, influencing its mobility, (2) high levels of CRM1 inhibit Rev activity and (3) Rev is capable of recruiting other CRM1-interacting factors to nucleoli that are capable of inhibiting Rev activity [26, 58, 59]. This model will be investigated in future experiments.
The RNAi experiments suggest that endogenously expressed 16.4.1 gene products can also affect Rev function. As expected, the stimulatory effect of RNAi-mediated inhibition of 16.4.1 expression was small, since Rev is known to function efficiently in 293T cells . We attempted to study the long-term effect of inhibition of endogenous 16.4.1 on Rev function by establishing cell lines stably expressing siRNA against 16.4.1. However, this approach was not feasible because of cell death after 2–3 weeks of expression of 16.4.1 siRNAs. This indicates that 16.4.1 gene products are crucial for cell viability. On the other hand, overexpression of 16.4.1 is well tolerated as demonstrated by the establishment of a cell line stably expressing 16.4.1-GFP.
The physiological role of interaction of Rev with 16.4.1 is not clear yet and may be positive or negative, depending on the levels of expression of 16.4.1. At low levels 16.4.1 proteins may act as a molecular chaperones of Rev, counteracting the strong tendency of Rev to aggregate with itself and/or preventing incorrect interactions with other cellular proteins. The occurrence of cytoplasmic cellular factors that inhibit Rev multimerization is suggested by a recent report demonstrating only weak Rev-Rev interaction in the cytoplasm of living cells . At high concentrations, 16.4.1 may decrease transactivation function of Rev, for example by sequestering Rev in inactive complexes in nucleoli. Inactivation of Rev by 16.4.1 could play a role in protecting the cells from Rev-mediated cytotoxicity .
HIV-1 infection of human cells involves various interactions between cellular and viral factors (reviewed in ). Some cell types (e.g. astrocytes) can control HIV-1 replication demonstrating the impact of cellular factors on HIV infection [60, 64]. Identification of cellular factors that are able to interfere with viral replication will contribute to understanding of cellular defence mechanisms against viral intruders and may also lead to identification of new targets for therapeutic approaches for virus restriction. Using HIV-1 Rev as "bait" we were able to identify a previously undescribed cellular interaction partner of an HIV-1 protein, 16.4.1. The 16.4.1 protein is exported from the nucleus by CRM1 and accumulates in the cytoplasm. An important feature of 16.4.1 is its ability to impair transactivation capacity of Rev, although both proteins localize to different cellular compartments. Conversely, Rev is capable of affecting localization of the 16.4.1 protein by recruiting it to the nuclei/nucleoli of eukaryotic cells. Because of its properties we suggest naming the 16.4.1 protein "Risp" (Rev interacting shuttling protein).
Data base analyses and preliminary studies with a specific monoclonal antibody suggest that human proteins with Risp sequences are expressed in various human cell types including HIV-1 target cells. The objective of future studies will be to characterize Risp proteins, their cellular functions and their influence on Rev activity and HIV-1 replication in different HIV-1 target cells.
This study represents a further step toward elucidating the network of host cell factors that interact with the HIV-1 Rev protein and influence its functions. This study also illustrates the power of viral proteins as tools for identification and biological characterization of novel cellular factors. Use of similar experimental strategies as presented here will help to gain deeper understanding of virus-cell interactions.
The inserts of all plasmid constructs used in this study were verified by sequence analysis (Sequiserve, Germany).
Expression plasmids for yeast two-hybrid analysis
pEG202  was used to express bait proteins containing various Rev sequences fused to the LexA DNA binding domain in yeast. pEG202 also expresses the yeast selection marker His3.
For construction of pEG202-sRev, pBsRev [64, 66] was used as template for PCR amplification to generate the rev sequence of HIV-1 isolate HXB2; the PCR product was inserted into the EcoRI site of pEG202. The same procedure was used for construction of bait plasmids pEG202-RevM4, pEG202-RevM10BL, pEG202-RevM5, pEG202-RevSLT40, using pcTat-RevM4 , pCsRevM10BLsg143 , pcRevM5  and pcRevSLT40  as PCR templates. Bait plasmids used as controls for unspecific interaction contained the wildtype rev sequence in antisense orientation (pEG202-sRev(antisense)) or encode a bait protein unrelated to Rev (pEG202-LexCD2).
pJG4-5 and its derivative pJG4-6 was used for galactose-inducible expression of prey proteins containing the protein of interest fused to the NLS of SV40 T antigen, and the B42 transcriptional activator . pJG4-5/6 also express the yeast selection marker Trp1.
pSH18-34 reporter plasmid contains 8 LexA operators that direct expression of the lacZ reporter gene.
The expression library used for two-hybrid screening contained cDNAs from human Jurkat T-cells inserted into the EcoRI/XhoI sites of pJG4-5 .
For mapping of Rev-interacting regions of 16.4.1, sequences encoding full-length 16.4.1 or various fragments of 16.4.1 were generated by PCR amplification using pC16.4.1sg143 as template and primers adding a 5' MluI site and a 3' NotI site. PCR products were inserted into pJG4-6 cleaved with MluI and NotI.
Plasmids pJG4-5, pJG4-6, pEG202, pSH18-34 and the Jurkat T-cell cDNA expression library were kindly provided by Waldemar Kolanus, University of Bonn, Germany.
Expression plasmids for mammalian two-hybrid analysis
Protein interactions in human cells were analysed with the CheckMate™ Mammalian Two-Hybrid System (Promega, Madison, USA), which uses pACT and pBIND vectors and the G5luc reporter plasmid. pACT and pBIND direct expression of fusion proteins containing the transcriptional activation domain of Herpes virus simplex VP-16 (pACT) or the Gal4 DNA binding domain (pBIND) at the N-terminus and potential interactor domains at the C-terminus. pG5luc contains 5 Gal4-binding motifs and a minimal promoter for inducible expression of the firefly luciferase reporter gene.
pACT and pBIND expression plasmids were constructed by PCR amplification of coding sequences from plasmid templates with primers adding restriction sites for insertion into the multiple cloning regions of the target vectors. The rev sequence was generated from pEG202-sRev and inserted into the SalI site of pACT (pACT-Rev). 16.4.1 sequence was generated from clone DKFZp434O171Q (RZPD, Berlin, Germany;) and inserted into the BamHI sites of pACT and pBIND (pACT-16.4.1 and pBIND-16.4.1). The human CRM1 sequence was amplified from pChCRM1sg143  and inserted into the BamHI site of pACT (pACT-hCRM1).
Plasmids encoding GFP-tagged proteins
The vector pFRED143 contains a humanized version of a strong fluorescent GFP mutant (sg143) under the control of the CMV immediate early promoter . pC-sg143 plasmids were constructed by using the cloning strategy described in , involving insertion of protein coding sequences without translational start and stop codons in frame with gfp-sequences into pFRED at a unique NheI site located immediately downstream of codon 1 of the GFP ORF.
The 16.4.1 sequence in pJG4-5 contains a 163 amino acid reading frame which is terminated by a stop codon but lacks an initiation codon. A potential translational initiation codon was identified 24 nucleotides upstream of and in frame with the 16.4.1 sequence in a human fetal cDNA (Gene Bank Accession Nr. W67699). For construction of pC16.4.1sg143, the 16.4.1 sequence in pJG4-5 was amplified with a 5' primer incorporating sequences encoding amino acids 2–7 into the PCR product, which was inserted into the NheI site of pFRED143. Sequence analysis of pC16.4.1sg143 verified formation of a single open reading frame by 16.4.1 and GFP sequences, resulting in expression of a protein with a predicted molecular mass of approximately 46 kDa.
For construction of pC16.4.1(74–133)sg143 the sequence encoding amino acids 74 to 133 was generated by PCR from pC16.4.1sg143 and inserted into the NheI site of pFRED143. pC16.4.1(74–133)2sg143 contains tandem sequences encoding amino acids 74 to 133 of 16.4.1.
The plasmid pC16.4.1(NESmut)sg143 expresses a mutant 16.4.1-GFP fusion protein in which L92, I97 and I99 within the core nuclear export of 16.4.1 are replaced by alanine residues. pC16.41(NESmut)sg143 was constructed by PCR based site directed mutagenesis from pC16.4.1sg143.
For construction of pCRev(52–116)sg143, sequences encoding amino acids 52–116 of Rev were amplified from pCsRevsg143  and inserted into the BspEI site of the pFRED143 variant pFRED143BspEI.
pCPKIα sg143 directs expression of GFP-tagged human PKI and was constructed as described in .
Plasmids encoding IgG1-tagged proteins
pIg was kindly provided by Waldemar Kolanus and was used for construction of plasmids directing expression of 16.4.1 fusion proteins with an N-terminal IgG1 tag. pIg contains CH2 and CH3 domain segments from human IgG1 cDNA in the mammalian expression vector pRK5 [67, 70]. Sequences encoding 16.4.1 or various regions of 16.4.1 were amplified from pC16.4.1sg143. PCR products were inserted into the MluI/NotI sites of the pIg polylinker, resulting in construction of the following plasmids: pIgG1-16.4.1, pIgG1-16.4.1(2–38), pIgG1-16.4.1(2–133), pIgG1-16.4.1(38–171), pIgG1-16.4.1(74–171) and pIgG1-16.4.1(134–171).
Plasmids used in Rev activity assay
pLRed(INS)2R reporter plasmid was constructed in a similar manner as described for pLRed(p17/p24INS)R . Briefly, a DNA fragment with HIV-1 gag-sequences containing INS 1 and 2 (nucleotides 379–1424 of the HXB2 genome) was isolated from pB37R  by Cla I-digestion and inserted into Cla I-digested and dephosphorylated pLRedR . pLRed(p17/p24INS)R contains two copies of the HIV-1 gag sequences in sense orientation. This plasmid directs Rev and Tat-dependent expression of red fluorescent protein (RFP).
pL3Tat contains the HIV-1 tat gene under the control of the HIV-1 LTR .
pCsRev-CFP was established by replacement of the GFP encoding sequence of pCsRevsg143 mentioned above with the coding sequence for cyan fluorescent protein (CFP).
Yeast two-hybrid assay
The yeast interaction trap was performed essentially as described in [65, 67], using yeast strain EGY48 which contains the LEU2 gene under the control of LexA operators and has defective leu2, his3, trp1 and ura3 genes. The pEG202-sRev bait plasmid was transformed into yeast strain EGY48 bearing the pSH18-34 reporter plasmid. This "bait" strain was transformed with the Jurkat T-cell library contained in the yeast expression plasmid pJG4-5. Criteria for protein-protein interactions were growth on medium containing galactose and lacking uracil, histidine, tryptophane and leucine, no growth in the same medium containing glucose instead of galactose, and expression of beta-galactosidase. Forty-six yeast transformants were obtained that grew on selective medium and expressed the lacZ reporter gene. cDNAs from those yeast clones were purified by passaging through E. coli KC8 and retested for interaction with pEG202-sRev but not with control bait plasmids pEG202-sRev(antisense) and pEG202-LexCD2 to confirm specific interaction.
Mammalian two-hybrid assay
Mammalian two-hybrid assay was performed in HEK293 cells, using the CheckMate™ Mammalian Two-Hybrid System (Promega, Madison, USA).
HEK293 cells were cotransfected with pBIND and pACT constructs for expression of VP16 and Gal4 proteins fused to potential interactor domains (1 μg each) and with the pG5luc reporter plasmid (2 μg). For each interactor assay, parallel transfections were performed with G5luc and pBIND and pACT vectors expressing Vp16 and Gal4 without interactor domains to determine background expression of the luciferase gene. Two days after transfection cells were lysed, and firefly luciferase activity (in relative light units per second, RLU/s) quantified using the Luciferase Reporter Assay System (PROMEGA) and the ORION I Microplate Luminometer (Berthold Detection Systems, Pforzheim, Germany). The total amount of protein in cell lysates was quantified using the BCA Protein Assay Reagent Kit (Pierce Chemical Co., Rockford, USA) and luciferase activity (RLU/s) standardized to 1mg of total protein in the cell lysate. Values are expressed as fold-induction of luciferase activity over basal expression levels.
Cell culture, transfection and Leptomycin B treatment
HeLa and HEK293 cells were maintained in Dulbecco's Modified Eagle Medium containing 2 nM Glutamax I (Life Technologies, Karlsruhe, Germany) and 10% fetal calf serum (Seromed, Berlin, Germany).
All transfection experiments were performed in 35-mm-diameter dishes (BD Biosciences, Bedford, MA, USA). Cells were seeded at a density of 1 × 105 cells per dish one day prior to transfection and cultured for 24 h after transfection. HEK293 cells were transfected by calcium phosphate coprecipitation using the CellPhect kit (Pharmacia, Freiburg, Germany). Transfection of HeLa cells was performed with the FuGENE™6 Transfection Reagent (Roche Diagnostics, Mannheim, Germany) using 500 ng plasmid DNA per dish.
Leptomycin B (LMB, Sigma-Aldrich, Munich, Germany) treatments were performed 24 hours after transfection at a concentration of 5 nM LMB for 2 hours. For microinjection experiments, LMB was added at a concentration of 10 nM 2 hours prior to injection.
For evaluation and quantification of fluorescence, cells were fixed with 4% paraformaldehyde (PFA) for 30 minutes at room temperature; nuclei were stained with Hoechst 33343 (Molecular Probes Europe BV, Leiden, Netherlands) for 10 minutes.
Toxic influences of long-term expression of 16.4.1-GFP in HeLa cells were assessed by CytoTox-ONE™ and CellTiter-Glo™ cell viability assays (Promega, Madison, USA) according to manufacturer's instructions.
The cell line HeLa 16.4.1-GFP expresses 16.4.1-GFP constitutively and was established by transfection with pC16.4.1sg143 followed by G418-selection (80 μg/ml). Non-fluorescent antibiotic resistant cells were excluded by FACS sorting.
Cells were fixed with PFA (4%) and permeabilized with TritonX-100 (0.2%).
IgG1-16.4.1 fusion proteins were detected by direct staining with a Cy3-conjugated goat anti-human IgG1 antibodies (Dianova, Hamburg, Germany), diluted 1:200 in phosphate buffered saline containing 1% BSA. For detection of 16.4.1 antigens, a monoclonal antibody against 16.4.1 (Kramer-Hämmerle et al. in preparation) was used as primary antibody and a Cy3-conjugated goat anti-rat antibody (Dianova, Hamburg, Germany) as secondary antibody.
Whole cell lysates were prepared with RIPA buffer containing protease inhibitors (protease inhibitor cocktail; Sigma, Taufkirchen, Germany) and separated on either precast 4–12% Bis-Tris or 3–8% Tris-Acetate gradient gels (Invitrogen, Karlsruhe, Germany). After transfer onto nitrocellulose membranes, proteins were probed with primary polyclonal rabbit antibodies against GFP (Chemicon Europe, Hofheim, Germany) or with the 16.4.1-specific monoclonal antibody and with HRP-conjugated secondary goat anti-rabbit or anti-rat antibodies (Dianova, Hamburg, Germany). Protein bands were detected by an enhanced chemiluminescence system (Perbio Science, Bonn, Germany).
Quantitative fluorescence microscopy
Microscopy of cells expressing fluorescent proteins and quantitative analysis of subcellular distribution of fluorescence was performed as described . Images for quantification were taken at 32-fold magnification with flexible exposure times and evaluated by IPlab software for fluorescence values below pixel saturation. Each cell group was photographed as phase contrast and fluorescence images for Hoechst 33342 and GFP.
3D deconvolution and widefield multichannel unmixing microscopy
Fluorescence microscopic imaging, 3D deconvolution and widefield multichannel unmixing was carried out with a computer-controlled Zeiss Axiovert 200 M research microscope ("Cell Observer") with scanning stage (Carl Zeiss, Goettingen, Germany) and Software AxioVision 4.2 (Carl Zeiss Vision, Hallbergmoos, Germany). Images of 2% PFA-fixed specimen were acquired using a Zeiss 40×/1.3 Plan Neofluar objective and Zeiss filter sets No. 44 (GFP), 49 (Hoechst33342) and 47 (CFP). Z-stacks with 100 optical sections at 0.325 μm intervals were captured with a Zeiss AxioCam HRm CCD-Camera with full resolution of 1388 × 1044 pixels. Deconvolution of fluorescence images  was carried out with AxioVision 4.2 software using a constrained iterative algorithm and auto linear normalization. Subsequently widefield multichannel unmixing was performed on the deconvolved image-stacks to correct for fluorescence bleedthrough of Hoechst-, CFP- and GFP- signals. Three reference samples with either one of the three fluorochromes were prepared, reference measurements were performed and a 3 × 3 matrix was generated that was used to unmix the sample image-stack. Processed images were then arranged for presentation and exported with AxioVision 4.2 software.
Compounds for microinjection were generated and microinjections were performed as described previously [49, 51]. Briefly, bovine serum albumin (BSA) was first labeled with Alexa-red (excitation 568 nm, Molecular probes) and subsequently conjugated to the following peptides:
(1.) HIV-1 Rev-NES represented by -CGG-LQPPLERLTLD and (2.) 16.4.1 amino acids 86 to 105 -CG-KTLESNLFDDNIDIFADLTV. Both peptides were synthesized by Sigma-Genosys. For coinjections unconjugated BSA labeled with Alexa-green (excitation 488 nm, Molecular probes) was used. Peptide solutions with a concentration of 1 mg/ml were injected into the nuclear compartment of HeLa cells. Two hours after injection, cells were fixed with 4% PFA, images of green and red channels were taken and the percentages of red (BSA-conjugated peptides) and green (unconjugated BSA) fluorescent signals were determined. The ratio between red and green fluorescence in the nuclear and cytoplasmic compartments indicates the relative translocation activity of the peptide.
Rev activity assay
The Rev activity assay was performed essentially as described previously . Transfections were performed in 6-well plates using FUGENE™6 according to the manufacturer's protocol with 1 μg pLRed(INS)2R, 0.2 μg pL3Tat, 0.1 μg pCsRev-CFP and 0.1 μg pFRED143. To evaluate the influence of 16.4.1-GFP on Rev-activity, pC16.4.1sg143 (0.5 μg or 1 μg) was added to the transfection mixture. Expression of fluorescent fusion proteins was checked by microscopy 24 hours after transfection and cells were analysed by flow cytometry. Typically 100,000 cells of each transfected well were analysed with a Becton Dickinson FACSCalibur flow cytometer equipped with a 488 nm argon laser and controlled by the software CellQuest Pro. GFP-fluorescence was analysed in channel FL-1 and RFP-fluorescence in FL-3. The percentage of reporter positive cells (RFP) in the transfected cell population (GFP) was determined.
RNAi interference in combination with Rev activity assay
For down-regulation of gene expression, 16.4.1 specific (siRNA-16.4.1) and non-specific (siRNA-nsp) and a GFP-specific siRNA were designed and synthesized by Qiagen. Transfections were carried out using RNAiFect transfection reagent (Qiagen) according to supplier's protocol. Target cells were seeded one day prior to transfection in 6-well plates, 5 μg siRNA per well were used for each transfection. Silencing effects of GFP-fusion proteins were determined by FACS analyses 48 hours after transfection. For the combined RNAi-Rev activity experiments, siRNA transfection was performed 24 h prior to DNA transfection.
Similarities between nuclear export signals in the NES database NESbase 1.0  were analysed with a set of amino acid weight matrices adapted from the MatInspector algorithm  using the BLOSSOM similarity matrix values to account for conserved amino acid substitutions. Reading frames were predicted with the tool ATGpr .
Statistical analysis of data was carried out with the GraphPadPRISM program (GraphPad Software Inc., San Diego, CA, USA). Significances of differences between data sets were determined by calculating two-tailed P values by the Mann-Whitney U test.
We are very grateful to Waldemar Kolanus, University of Bonn, for providing us with the yeast strain, plasmids and cDNA library for yeast two-hybrid analysis and with the mammalian expression plasmid pIgG1. We thank Alexandra Ludvigsen for her contributions to the yeast two-hybrid screening, Kerstin Lux and Andrea Brebeck for cloning of the M2H constructs and the Rev-CFP expression plasmid and Petra Willnauer for performing microinjections. We are especially grateful to Elisabeth Kremmer for establishment of the rat monoclonal antibody against 16.4.1. We thank Kerstin Cartharius (Genomatix) for adapting the MatInspector algorithm to protein sequences.
This work was partially supported by the Deutsche Forschungsgemeinschaft (F.C.S.; SFB 464).
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