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BMC Bioinformatics

, 10:A7 | Cite as

Reconstructing the virulome of the human pathogen Streptococcus pyogenes using NMPDR subsystems-based annotation

  • Ramy K Aziz
  • Leslie K Mcneil
Open Access
Meeting abstract
  • 2.4k Downloads

Keywords

Gene Regulatory Network Strain Emergence Bioinformatics Resource Genomic Revolution Streptococcal Strain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Background

The increasing number of published complete microbial genomes has revolutionized biological sciences and is driving a paradigm shift in microbiology. While this genomic revolution has made the reconstruction of an organism's metabolism from genomic data achievable, predicting the pathogenic potential of host-associated microbes is still in its early stages; hence, developing innovative bioinformatics tools that integrate microbiologists' expertise and experimental laboratory data with sequence data remains a necessity. For this purpose, the NIH-funded National Microbial Pathogen Data Resource (NMPDR, http://www.nmpdr.org) was established as a bioinformatics resource center for specific bacterial pathogens, including staphylococci, streptococci, and sexually transmitted bacteria [1]. Genomes in NMPDR are annotated by the recently developed subsystems annotation technology [2, 3], available from the SEED environment http://theseed.uchicago.edu/FIG/index.cgi. This technology relies on analyzing genes in their chromosomal context and combines the accuracy of human curation with the speed of automated propagation [2].

Methods and results

In this study, we apply the subsystems annotation technology to reconstruct the virulome of the human pathogen Streptococcus pyogenes that claims 500,000 lives every year [4], and causes a wide range of diseases that affect adults and children [5]. In particular, we use NMPDR tools for pathogenomic comparison of the fully sequenced streptococcal serotypes, and highlight the impact of prophages and highly recombinatorial genomic segments, including the newly discovered pilus locus [6], on streptococcal strain emergence and diversification [7]. Our analysis defines the core and dispensable elements of the streptococcal virulome, which includes – in addition to the ancestral, species-specific virulence proteins – the phage-encoded toxins and their pseudogenes. Finally, we use comparative analysis of streptococcal subsystems in context of actual transcriptome data to gain insight into the complex gene regulatory networks that control virulence.

References

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Copyright information

© Aziz and Mcneil; licensee BioMed Central Ltd. 2009

This article is published under license to BioMed Central Ltd.

Authors and Affiliations

  1. 1.Department of Microbiology and Immunology, Faculty of PharmacyCairo UniversityCairoEgypt
  2. 2.Computation InstituteUniversity of ChicagoChicagoUSA
  3. 3.National Center for Supercomputing ApplicationsUniversity of IllinoisUrbanaUSA

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