Ca2+ binding to complement-type repeat domains 5 and 6 from the low-density lipoprotein receptor-related protein
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The binding of ligands to clusters of complement-type repeat (CR)-domains in proteins of the low-density lipoprotein receptor (LDLR) family is dependent on Ca2+ ions. One reason for this cation requirement was identified from the crystal structure data for a CR-domain from the prototypic LDLR, which showed the burial of a Ca2+ ion as a necessity for correct folding and stabilization of this protein module. Additional Ca2+ binding data to other CR-domains from both LDLR and the LDLR-related protein (LRP) have suggested the presence of a conserved Ca2+ cage within CR-domains from this family of receptors that function in endocytosis and signalling.
We have previously described the binding of several ligands to a fragment comprising the fifth and the sixth CR-domain (CR56) from LRP, as well as qualitatively described the binding of Ca2+ ions to this CR-domain pair. In the present study we have applied the rate dialysis method to measure the affinity for Ca2+, and show that CR56 binds 2 Ca2+ ions with an average affinity of KD = 10.6 microM, and there is no indication of additional Ca2+ binding sites within this receptor fragment.
Both CR-domains of CR56 bind a single Ca2+ ion with an affinity of 10.6 microM within the range of affinities demonstrated for several other CR-domains.
KeywordsDialysis Time Average Affinity Receptor Fragment Backbone Carbonyl Group LDLR Family
complement-type repeat domain
epidermal growth factor
low-density lipoprotein receptor
The understanding of how LRP binds Ca2+ ions is important. The binding of all ligands is dependent on the presence of Ca2+ [12, 13, 14] and ligand dissociation within the endocytic pathway has been suggested to occur as a possible consequence of the decrease in pH and the accompanying loss of affinity for Ca2+ in the acidic endosomes .
Ligand recognition requires key residues in the CR-domains of the LDLR-like proteins as well as the presence of Ca2+ ions. One important residue for this interaction is located at the center position between the fourth and the fifth cysteine residue, where an acidic side chain is important for the efficient recognition of multiple protein ligands [15, 16]. The backbone carbonyl group of residues located at identical positions in several domains homologous to CR5 and CR6 is involved in the coordination of a Ca2+ ion [5, 6]. The coordination sphere of the bound Ca2+ ion is well defined in octahedral geometry, with four carboxylate oxygen atoms from the acidic motif in one plan and two carbonyl oxygen atoms completing the coordination sphere at the apices (Figure 1B).
We have previously demonstrated the specific binding of LRP ligands to a minimum receptor fragment comprising only 2 CR-domains , and focused on the ligand interaction with the tandem domain CR56 fragment [17, 18]. The affinity for a bound Ca2+ ion has been reported for several CR-domains, and in order to better understand the Ca2+-dependent ligand binding properties of CR56 we have determined the stoichiometry and affinity for Ca2+ binding to this CR-domain pair. Furthermore, we undertook a stringent/direct method of affinity determination using the microchamber rate dialysis method  independent of the local molecular environment at the Ca2+ localization site, and could compare the determined affinity with data obtained by less direct methods, e.g. fluorescence analysis. We conclude that in general the affinities do not vary significantly among the CR-domains investigated.
Results and Discussion
The demonstration of Ca2+ binding to the ubiquitin-fused CR56 protein  strongly suggested that CR56 contains at least one efficient Ca2+ binding site similar to other CR-domains [1, 5]. However, from the previously adopted approach we could not determine the stoichiometry and affinity between Ca2+ and CR56. This was the main objective of the current study. Furthermore, we wanted to test whether the coupling of a CR-domain to a neighboring domain would influence the affinity of the single CR-domain, compared to the increasing pool of data describing the binding of Ca2+ to isolated CR-domains [6, 20, 21, 22, 23]. After affinity purification of ubiquitin-fused CR56 containing the authentic amino acid sequence, we liberated the CR56 domain pair from its fusion partner by factor Xa cleavage (as described in ref. ). 2D gel analysis showed a high degree of purity, since the recombinant CR56 protein migrated as a single major spot (not shown). Ca2+ binding was measured using the microchamber rate dialysis technique [19, 24].
Ca2+ binding properties of various CR-domains
Ca2+ affinity(KD) μM
pH 7.4, 30°C
pH 5.0, 30°C
pH 7.4, 30°C
The present data
pH 7.4, 37°C
The present data
pH 7.4, 37°C
pH 7.4, 30°C
pH 7.4, 30°C
pH 7.4, 30°C
The Ca2+ ions in the hitherto solved domain structures are located in identical Ca2+ cages as for CR5LDLR described previously by Fass and colleagues  (Figure 1B). From a high level of sequence conservation for the sequences of CR5LRP and CR6LRP compared to the sequences of the CR-domains with a solved domain structure (Figure 1C), we suggest that the binding site for Ca2+ within both CR5 and CR6 are very similar to these. This is very important information for the assignment of nuclear magnetic resonances for the solution structure determination of CR56 (ongoing project).
The demonstration of independent folding of each CR-domain in tandem CR-domain pairs is substantiated by the reports of a total lack of interdomain interactions, and that Ca2+ coordination does not involve chelates from adjacent CR-domains [27, 28]. In line with this our data suggest that two and only two Ca2+ are bound per tandem fragment as also reported for domain pairs from LDLR comprising CR1LDLR-CR2LDLR and CR5LDLR-CR6LDLR [25, 27]. Since the Ca2+ affinity for CR5 and CR6 is similar to other known binding sites, it is tempting to believe that the Ca2+ ion is located in a similar Ca2+cage as for these domains [1, 5, 6], and therefore the chelating residues located at identical positions within the primary structure is also cation coordinating in these domains. If this indeed is the case, we have recently demonstrated that the two residues speculated to provide electrons for Ca2+coordination via their backbone functional group (Trp-953/Asp-958 in CR5 and Trp-994/Asp-999 in CR6) both contribute significantly to ligand binding [17, 18]. The possibility that Ca2+ most likely are intimately linked to these residues suggest that Ca2+ binding exerts influence on ligand binding to CR-domains, because of a lack of dynamic and flexibility of residues at this particular position. In addition, especially the acidic residues is also involved in the intramolecular binding of CR-domains to the EGF-precursor homology domain at low pH, speculated to result in structural rearrangement and ligand release within endosomes, underscoring the importance of understanding the local environment around the Ca2+ binding site .
Both CR-domains of the CR56-domain pair bind a single Ca2+ ion with an average affinity, KD~10.6 μM.
Production and RAP-affinity purification of the ubiquitin-fused CR-domain pair comprising the fifth and the sixth CR-domain from LRP (see Figure 1) was described previously . Purity was verified by two-dimensional gel electrophoresis showing the sample to be highly homogeneous (not shown).
Calcium binding analysis
Qualitative 45Ca blotting analysis to ubiquitin-fused CR56 has been described . The quantitative rate dialysis method  was applied to determine the Ca2+ binding constants for CR56. The binding experiments were performed in a medium containing 10 mM HEPES pH. 7.0, 150 mM NaCl and a final CR56 concentration at 15 μM. Buffers and protein solutions were passed through a Chelex 100 column (BioRAD) in order to obtain cation free solutions before use. The resin was pre-equilibrated with HEPES binding buffer before use. We have previously shown that this procedure is able to bring the Ca2+ content of the solutions to negligible levels as determined by atomic absorption spectrometry . The dialysis membrane was of cellulose, Type 14.10, molecular cut-off 5000, from Diachema (Munich, Germany). The following equation was used to calculate the free Ca2+ concentration from the total Ca2+ concentration:
[Ca2+]free = - [Ca2+]total [k*(t+t0)]-1 ln [(Aleft - Aright)/(Aleft + Aright)]
where k is a pre-determined rate constant, t is time of dialysis and t0 is an experimentally determined value which is dependent upon the procedure of filling, withdrawal, and rinsing of chambers and varies with the substance dialysed . Aleft and Aright denote the radioactivity in the left and right solution, respectively, after dialysis measured by liquid scintillation counting in an LKB Wallac 1218 Rackbeta scintillation counter. The values of k and t0 for the Ca2+ ligand used were 0.04650 min-1 and 0.18 min. They were determined in separate experiments with no protein present and using varying dialysis times as described in detail . In short, values of ln [(Aleft - Aright)/(Aleft + Aright)] are plotted versus the dialysis time. The rate constant k is then determined from the slope of the curve while t0 is determined as the numerical value of the intercept with the time-axis (x-axis). The average number of Ca2+ ions bound per protein molecule, r, was calculated from
r = ([Ca2+]total - [Ca2+]free)/ [CR56]
Under the presumption that CR56 contains a number, n, of identical and independent Ca2+-binding sites the binding isotherm was fitted to the Scatchard equation by linear regression:
r/ [Ca2+]free = - r/KD + n/KD,
where KD is the dissociation constant.
We thank Kirsten Peterslund for excellent technically assistance. This work was supported by Grant 9901730 from the Danish National Science Research Council (HCT), the Danish Medical Research Council and the Novo Nordisk Foundation.
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