PDIL1-2 can indirectly and negatively regulate expression of the AGPL1 gene in bread wheat
ADP-glucose pyrophosphorylase (AGPase), the key enzyme in plant starch biosynthesis, is a heterotetramer composed of two identical large subunits and two identical small subunits. AGPase has plastidial and cytosolic isoforms in higher plants, whereas it is mainly detected in the cytosol of grain endosperms in cereal crops. Our previous results have shown that the expression of the TaAGPL1 gene, encoding the cytosolic large subunit of wheat AGPase, temporally coincides with the rate of starch accumulation and that its overexpression dramatically increases wheat AGPase activity and the rate of starch accumulation, suggesting an important role.
In this study, we performed yeast one-hybrid screening using the promoter of the TaAGPL1 gene as bait and a wheat grain cDNA library as prey to screen out the upstream regulators of TaAGPL1 gene. And the barley stripe mosaic virus-induced gene-silencing (BSMV-VIGS) method was used to verify the functional characterization of the identified regulators in starch biosynthesis.
Disulfide isomerase 1-2 protein (TaPDIL1-2) was screened out, and its binding to the TaAGPL1-1D promoter was further verified using another yeast one-hybrid screen. Transiently silenced wheat plants of the TaPDIL1-2 gene were obtained by using BSMV-VIGS method under field conditions. In grains of BSMV-VIGS-TaPDIL1-2-silenced wheat plants, the TaAGPL1 gene transcription levels, grain starch contents, and 1000-kernel weight also significantly increased.
As important chaperones involved in oxidative protein folding, PDIL proteins have been reported to form hetero-dimers with some transcription factors, and thus, our results suggested that TaPDIL1-2 protein could indirectly and negatively regulate the expression of the TaAGPL1 gene and function in starch biosynthesis.
KeywordsAGPase BSMV-VIGS Protein disulfide isomerase Starch biosynthesis Triticum aestivum L. Yeast one-hybrid
barley stripe mosaic virus-induced gene-silencing
wheat disulfide isomerase 1-2 protein
- LSU or AGPL
large/modulatory subunits of AGPase
- SSU or AGPS
small/catalytic subunits of AGPase
International Wheat Genome Sequencing Consortium
quantitative real-time PCR
glyceraldehyde 3-phosphate dehydrogenase
one-way analysis of variance
open reading frame
Bread wheat is one of the most important cereal crops, accounting for approximately 20% of the daily calorie consumption worldwide, and starch is composed of approximately 70% wheat grains and is the most abundant storage carbohydrate in this organ . AGPase uses the substrates glucose-1-phosphate (G-1-P) and ATP to produce pyrophosphate and ADP-glucose, the starch precursor, and is thought to be the key enzyme in starch synthesis . In plants, AGPase is a heterotetramer composed of two large/modulatory subunits (LSU, or AGPL) and two small/catalytic subunits (SSU or AGPS) . According to its cellular localization, AGPases have cytosolic and plastidial isoforms and these two types function independently to produce starch in cytosol and plastid, respectively. Thus, in plant cells, there are four types of AGPase subunits: cytosolic SSU, plastidial SSU, cytosolic LSU and plastidial LSU . In the plant cells of most dicotyledonous plants, AGPase is active exclusively in plastids, but it is mainly present in the cytosol in the grain endosperm of the grass family, demonstrating approximately 85%, 90%, and 95% of the total AGPase activity in barley, rice, and maize, respectively [5, 6, 7], suggesting a crucial role of cytosolic AGPase in starch synthesis in these species.
The number of AGPase subunits is species-specific. For example, four AGPase large subunits (AGPL1, AGPL2, AGPL3, and AGPL4) have been identified in rice and maize, only two subunits, AGPL1 and AGPL2, are present in barley, and the extents to which they play roles in starch biosynthesis are different [8, 9]. Our previous studies have demonstrated that the expression of the TaAGPL1 gene encoding the cytosolic large subunit of wheat AGPase shows similar changes to the rate of starch accumulation, and its transcripts in wheat grains are significantly and positively related to the activity of AGPase and the accumulation rate of grain starch . Moreover, overexpression of TaAGPL1 markedly enhanced AGPase activity and the rate of starch accumulation in wheat grains . These data demonstrate that TaAGPL1 plays an important role in AGPase enzyme and starch biosynthesis in bread wheat and could have potential applications in wheat breeding to develop high-yield wheat cultivars . However, the upstream regulatory mechanism of the TaAGPL1 gene family remains elusive.
Here we used the TaAGPL1 promoter as bait to screen an immature wheat grain cDNA library using a yeast one-hybrid (Y1H) assay to isolate upstream regulators. Among the identified proteins, one protein disulfide isomerase 1-2 (TaPDIL1-2) was selected to evaluate its binding to the TaAGPL1 promoter and test its potential function in regulating TaAGPL1 expression and starch biosynthesis.
Two semi-winter bread wheat cultivars, Zhoumai 18 and Bainong 207, characterized with high yield potentials, have widely been planted in the Yellow and Huai Valleys Winter Wheat Zone. The former was used in our previous studies [10, 12], and in this study, its leaf DNA was extracted to isolate the TaAGPL1-1D promoter and its grains were sampled for endosperm cDNA library synthesis and for detection of the TaAGPL1-1D promoter activity. The latter is relatively sensitive to plant viruses and was used for the BSMV-VIGS assay .
GUS activity of the TaAGPL1-1D gene assay
The sequence of TaAGPL1-1D gene was used as a query to search for its upstream promoter sequence in the International Wheat Genome Sequencing Consortium (IWGSC) database. A 1280-bp fragment was retrieved and used as its promoter sequence. A pair of primers were designed to amplify this fragment from cv. Zhoumai 18 (Additional file 1: Table S1), and then a series of nested 5′ deletions of this promoter were inserted into the pCAMBIA1301 vector containing the CaMV35S promoter to drive expression of the GUS gene, forming the Pro-1: GUS (− 1269 bp to + 11 bp), Pro-2: GUS (− 870 bp to + 11 bp), and Pro-3: GUS (− 483 bp to +11 bp) recombinant vectors, respectively. GUS activity of TaAGPL1-1D was assessed using a transient transformation system in N. benthamiana plants or immature wheat grains. In tobacco plants, the recombinant plasmids of Pro-1: GUS, Pro-2: GUS, and Pro-3: GUS were transferred into the Agrobacterium EHA105 lines, and then the EHA105 lines were coinfiltrated into the N. benthamiana leaves . For wheat grains, immature grains sampled at 20 days after anthesis were used to qualitatively and quantitatively determine the TaAGPL1-1D promoter activity using gene gun-mediated transient transformation protocol . The recombinant plasmids of Pro-1: GUS, Pro-2: GUS, and Pro-3: GUS were transfected using the PDS-1000/He Particle Delivery SystemTM (Bio-Rad, Hercules, CA, USA). Stained samples were photographed using a Canon Digital Camera EOS 70 D (Canon Co., Ltd., Zhuhai, China). Quantitative GUS activity was subsequently measured .
Identification of TaPDIL1-2 protein by Y1H screening
Approximately 2 mg of high-quality total RNA from wheat endosperm sampled at 5, 10, 15, 20, 25, 30, and 35 days after anthesis was mixed and extracted with TRIzol reagent (Invitrogen, Carlsbad, CA, USA). Wheat endosperm cDNA library was constructed using the Advantage 2 PCR Kit [Cat No. 639206, TaKaRa Biotechnology Co., Ltd., Dalian, China]. The TaAGPL1-1D promoter was used to construct the bait vector (pAbAi-TaAGPL1). The pAbAi-TaAGPL1 vector was then linearized and integrated into the genome of yeast strain Y1HGold. The bait strain Y1HGold/TaAGPL1-pro-pAbAi survived on SD/-Ura medium. The plasmids of the wheat endosperm cDNA library were next transformed into competent cells of the bait strain, and the transformed yeast cells grew on plates of SD/-Leu, -Ura, +AbA100 at 30 °C for 2–4 days. The positive yeast colonies were isolated and confirmed by PCR amplification with 5′ T7-F and 3′ AD-R primers (Additional file 1: Table S1).
The interaction between TaPDIL1-2 protein and TaAGPL1-1D promoter was measured using another Y1H assay. Three fragments (Pro-1, 1280 bp, Pro-2, 881 bp and Pro-3, 494 bp) of the TaAGPL1-1D promoter were separately ligated into the pAbAi vector. ORF (open reading frame) sequence of TaPDIL1-2 gene was cloned and then ligated into the pGADT7 vector to produce the prey vector. Two concentrations (0 and 100 ng/mL) of AbA were used, and the TaPDIL1-2 prey vector was transformed into yeast Y1HGold strains containing the Bait-Reporter vectors. pGADT7-TaPDIL1-2 and pAbAi-TaAGPL1 promoters were co-transformed into the Y1HGold yeast strains. The transformed cells were grown on SD/-Leu, -Ura selective medium with 100 ng/mL AbA at 30 °C for 2–4 days.
Transcriptional expression data for the TaAGPL1-1D gene in grain, spike, leaf/stem at reproductive and vegetative stages were obtained from the expVIP database and then viewed as a heat map using the HemI-1.0 tool (Heatmap illustrator) .
To alleviate functional complementation and allow complete silencing, we selected a conserved cDNA fragment (190 bp) of the TaPDIL1-2 gene, three homoeologs of which shared 99.1% similarity, possibly enabling simultaneous silencing using the BSMV-VIGS method. The primers used for the BSMV-TaPDIL1-2 vector are shown in Additional file 1: Table S1. Viral vector construction, viral RNA transcription, and viral inoculation of BSMV-VIGS vectors were performed as described in our previous studies [13, 18]. BSMV-GFP-inoculated wheat seedlings were used as the negative control, and the appearance of chlorosis on the inoculated wheat spikes confirmed the successful BSMV inoculation . In the field, BSMV inoculation was performed in the spike of wheat plants at the heading stage, and a total of 142 and 167 spikes were inoculated with BSMV-TaPDIL1-2 or BSMV-GFP, respectively, using a 20-µL transcript mixture for each spike.
Transcription levels of the TaPDIL1-2 gene in the wheat grains at 19, 24, 29, and 34 days after anthesis were measured using the quantitative real-time PCR (qPCR) . The wheat Actin and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (GenBank accession no. AB181991 and EF592180) genes were used as internal controls.
One-way analysis of variance (ANOVA) was performed using SPSS version 17.0 software. Data are the mean ± standard deviation (SD) of at least three independent experiments. A multiple range test was used to compare the mean values at the P < 0.05 level.
Results and discussion
Isolation of the TaAGPL1 promoter
Bread wheat genome contains three closely related, yet distinct, subgenomes (AABBDD) and three homoeologs, with over 95% sequence identity in their coding regions for the majority of genes . In bread wheat, rice and maize, AGPL1 gene transcripts showed high levels in endosperm, whereas they were not detected in leaves or other organs [7, 8, 9, 12], suggesting that the promoter could be endosperm-specific. The cDNA sequence of the TaAGPL1 gene, which was highly expressed in endosperm during the grain filling period in our previous study , was searched against the recently published IWGSC databases (RefSeq v1.0) , and a high level (100% identities) to a chromosome-located contig (TraesCS1D01G427400.1) was found, suggesting its localization on 1D chromosome.
The binding between TaPDIL1-2 gene and TaAGPL1 promoter
Wheat grains were sampled at 5, 10, 15, 20, 25, 30, and 35 days after anthesis, endosperm was reserved and other organs were removed. The mRNA isolated from the sampled endosperm was used as template to synthesize the full-length double-stranded complementary DNA and together with the linearized pGADT7-Rec vector was co-transformed into yeast strain Y1HGold cells for directional combination through homologous recombination. The combined plasmids were extracted and subsequently transformed into E. coli strain stellar cells. The total capacity was 1.08 ± 0.3 × 106 cfu with more than 77.9% inserted fragments greater than 750 bp (Additional file 4: Fig. S3), which was suitable for yeast screening.
Characterization and expression of TaPDIL1-2
At the transcriptional level, the in silico expression data revealed three homoeologs of the TaPDIL1-2 gene that were more highly expressed during the reproductive stage than the vegetative stages, and during the reproductive stage, their expression level were higher in the endosperm/grain and spikes than in stem and leaf (Fig. 3c). The bread wheat endosperm mainly accumulates starch (≥ 70%) and protein (10–14%), forming the bulk of the grain. These findings suggest that TaPDIL1-2 homoeologs can function in starch biosynthesis in wheat grain.
Negative regulation of the TaPDIL1-2 gene in wheat starch biosynthesis
Gene function in higher plants is often explored through the use transgenic and mutational assays. In bread wheat, however, the multiple copy insertions, low transformation efficiency, cultivar specificity, time consumption, and high cost of transgenic approaches have greatly limited gene function studies in this species . The functional redundancy among homoeologs in this species also causes some difficulties in terms of generating null mutants . BSMV-VIGS method facilitates the rapid generation of gene knockdown phenotypes in polyploid species because plant transformation is not required, accelerating the characterization of target genes . In this study, this method was used to evaluate the function of the TaPDIL1-2 gene. We constructed BSMV-TaPDIL1-2 and BSMV-GFP vectors, and the latter was used as the control. To prevent functional complementation and allow complete silencing, we selected a conserved cDNA fragment (190 bp) of the TaPDIL1-2 gene, three homoeologs of which shared 99.1% similarity (Additional file 7: Fig. S5), potentially enabling the simultaneous silencing of its three homoeologs using the BSMV-VIGS method.
At 19, 24, 29, and 34 days after anthesis, the transcription levels of the TaPDIL1-2 gene were determined by using qPCR, and the β-actin and GAPDH genes were used as two internal controls. Our results showed that transcription levels of the TaPDIL1-2 gene in grains of BSMV-TaPDIL1-2-inoculated spikes were markedly decreased by 42.2–79.2% (Fig. 4b, c), similar to previous studies [13, 29]. The starch contents and 1000-kernel weight in grains of BSMV-TaPDIL1-2-inoculated wheat spikes were increased by 16.6% and 6.8%, respectively (Fig. 4d, e), demonstrating that TaPDIL1-2 silencing caused enhanced starch biosynthesis in the wheat endosperm. Thus, TaPDIL1-2 might act as a negative regulator of starch biosynthesis. Similarly, the transcription levels of the TaAGPL1-1D gene in the endosperm of BSMV-TaPDIL1-2-infected wheat spikes at the four sampling time points increased remarkably (Fig. 4f, g). Because our previous studies have confirmed the important role of the TaAGPL1 gene in starch biosynthesis in wheat grain , we inferred that the TaPDIL1-2 gene could play a crucial role in wheat starch biosynthesis by negatively regulating TaAGPL1-1D gene expression.
Indirect regulation of TaPDIL1-2 gene on expression of TaAGPL1-1D gene
Most of PDIL proteins contain a KDEL at the C-terminus, and are localized in ER, where they combine with other protein substrates to form enzymatic complexes, in which their classical thioredoxin domains are responsible for interacting with substrates . In the enzymatic complexes, PDIL proteins function as chaperones to bind to the unfolded or partially folded protein substrates by ascertaining their correct folding and preventing their aggregation . Cereal endosperm storage proteins (e.g., gliadins and glutenins) have many disulfide bonds, which are bound to some PDIL proteins .
We isolated the promoter of TaAGPL1-1DL gene and constructed a wheat endosperm cDNA library. Through a yeast one-hybrid screening by using the promoter of TaAGPL1-1DL gene as bait, and wheat grain cDNA library as prey, we further identified a protein disulfide isomerase (TaPDIL1-2), which bound to the promoter of TaAGPL1-1DL gene. By using BSMV-VIGS method in field experiments, we furthermore found that TaPDIL1-2 gene could negatively regulate the bread wheat starch biosynthesis.
JD performed experimental works and data analysis; YZ, YF and JW participated in data analysis; YW and QZ designed the experiments; SY and YW participated experimental works; GK and GL wrote the article; GL, GK and XO provided the financial support. All authors read and approved the final manuscript.
This study was financially supported by the National Natural Science Foundation of China (31871550 and 31571575), the Henan Province Tackling Key Problems in Science and Technology (182102110102), and the Important Science and Technology Specific Project of Henan Province (151100110700).
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The authors declare that they have no competing interests.
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