Correction to: Angiopoietin-1 receptor Tie2 distinguishes multipotent differentiation capability in bovine coccygeal nucleus pulposus cells
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Correction to: Stem Cell Res Ther (2016) 7:75
We have now fixed this issue by providing a new Fig. 2 using the reported Tie2 primary antibody from Bioss and the secondary antibody labeled with Alexa 488 (cat# A-11008, Molecular Probes, Life Technologies, Zug, Switzerland) for FACS sorting.
The corrected Material and Methods Section for this part on page 4 of the original article should read:
“Immunohistochemical staining for proteoglycans was performed by incubation of the sections with a monoclonal mouse anti-human proteoglycan antibody (10 μg/ml, clone EFG-4; Millipore, Billerica, MA, USA, diluted 1:50) overnight at 4 °C after permeabilization with 100 % methanol for 2 min, rehydration and blocking with 10 % FBS in phosphate buffered saline (PBS) for 1 hour. Secondary antibody was then added after stringent washing for 1 hour on the next day, which was a goat anti-mouse antibody (Alexa Fluor 555 goat anti-mouse SFX-Kit IgG A-31621, Invitrogen, Fisher-Scientific, Basel, 1:200 diluted). Finally, slides were mounted in DAPI containing embedding medium (Fluoroshield™ cat# ab104139, abcam plc, Cambridge, UK). Images were then taken with a confocal laser scanning microscope at a 10x magnification and using 4x4 tile imaging (cLSM710, Carl Zeiss, Jena, Germany).”
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