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RETRACTED ARTICLE: Diagnostic and prognostic value of plasma microRNA-195 in patients with non-small cell lung cancer

  • Keli Su
  • Tingcui Zhang
  • Yongrui Wang
  • Guijun HaoEmail author
Research

Abstract

Background

Recently, circulating microRNAs (miRNAs) have been reported to be stably detectable in plasma/serum and to function as potent biomarkers in various cancers. The aim of this study was to evaluate the expression level of plasma miRNA-195 in patients with non-small cell lung cancer (NSCLC) and investigate its diagnostic and prognostic value.

Methods

Quantitative real-time PCR was performed to evaluate plasma miRNA-195 levels in 100 NSCLC patients and 100 healthy volunteers. The association between miRNA-195 expression and clinicopathological factors as well as the overall survival was analyzed. Receiver-operating characteristic (ROC) curve analysis was carried out to assess the potential value of plasma miRNA-195 for NSCLC diagnosis.

Results

Plasma miRNA-195 was downregulated in NSCLC patients compared with healthy controls (P < 0.001). Decreased plasma miRNA-195 expression was significantly associated with lymph node metastasis and advanced clinical stage. ROC curve analysis showed that plasma miRNA-195 was a useful marker for NSCLC diagnosis. Multivariate Cox regression analysis confirmed low plasma miRNA-195 expression as an independent unfavorable prognostic factor for NSCLC patients.

Conclusions

These findings indicate that plasma miRNA-195 might serve as a promising biomarker for the early detection and prognosis evaluation of NSCLC.

Keywords

miRNAs Biological markers Non-small cell lung cancer Diagnosis Prognosis 

Abbreviations

AUC

Area under the curve

miRNA

MicroRNA

NSCLC

Non-small cell lung cancer

ROC

Receiver-operating characteristic

RR

Relative risk

RT-PCR

Reverse transcription-polymerase chain reaction

Notes

Acknowledgements

None.

Funding

No funds were received in support of this work.

Availability of data and materials

Our data will not be shared temporarily because further studies including these data are being performed.

Authors’ contributions

KS and GH conceived and designed the experiments. TZ and YW performed the experiments. TZ analyzed the data. GH contributed reagents/materials/analysis tools. KS and GH wrote the paper. All authors read and approved the final manuscript.

Competing interests

The authors declare that they have no competing interests.

Consent for publication

Not applicable.

Ethics approval and consent to participate

The Ethical Committee of The Fourth People’s Hospital of Jinan and The Central Hospital of Jinan approved this study protocol. Written informed consent was obtained from all participants.

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Copyright information

© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Authors and Affiliations

  • Keli Su
    • 1
  • Tingcui Zhang
    • 2
  • Yongrui Wang
    • 3
  • Guijun Hao
    • 1
    Email author
  1. 1.Department of OncologyThe Fourth People’s Hospital of JinanJinanChina
  2. 2.Department of Internal MedicineThe Central Hospital of JinanJinanChina
  3. 3.Department of Clinical LaboratoryThe Fourth People’s Hospital of JinanJinanChina

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