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Respiratory Research

, 20:207 | Cite as

Factors leading to failure to diagnose pulmonary malignant tumors using endobronchial ultrasound with guide sheath within the target lesion

  • Yoichi Nishii
  • Taro Yasuma
  • Kentaro Ito
  • Yuta Suzuki
  • Fumiaki Watanabe
  • Tetsu Kobayashi
  • Kota Nishihama
  • Corina N. D’Alessandro-Gabazza
  • Hajime Fujimoto
  • Esteban C. GabazzaEmail author
  • Fumihiro Asano
  • Osamu Taguchi
  • Osamu Hataji
Open Access
Research

Abstract

Background

The diagnostic yield of peripheral pulmonary lesions has significantly increased with the use of radial endobronchial ultrasound with guide sheath within the lesion. Here, we retrospectively evaluated factors leading to misdiagnosis of pulmonary malignant tumors using endobronchial ultrasound with the guide sheath within the lesion.

Methods

We assessed the final histopathological diagnosis of biopsy samples taken from 130 patients with lung malignant tumors that underwent endobronchial ultrasound with guide sheath within the lesion.

Results

Among 130 patients, 8 (6%) showed no definite malignant findings in biopsy samples but the presence of malignant cells (primary lung cancer 7, diffuse large B cell lymphoma 1) was subsequently confirmed by histopathological study of specimens taken by computed tomography-guided needle biopsy or surgery. Of the eight cases with diagnostic failure, the size of the biopsy sample was insufficient in five due to technical difficulties during the diagnostic procedure, and the diagnosis of malignant tumor was difficult in five cases because of extensive scarring tissue or central necrosis.

Conclusions

The results of this study showed that technical difficulties and/or pathological heterogeneity of the tumor might lead to failure to diagnose lung malignant tumor in cases using endobronchial ultrasound with guide sheath within the lesion.

Keywords

Bronchoscopy Diagnostic errors Lung Ultrasonography Neoplasms 

Abbreviations

adeno

Adenocarcinoma

CT

Computed tomography

DLBCL

Diffuse large B cell lymphoma

EBUS-i

Endobronchial ultrasound image

GS

Guide sheath

LtB

Left bronchus

MD

Moderately differentiated

PD

Poorly differentiated

R-EBUS

Radial endobronchial ultrasound

RtB

Right bronchus

SCC

Squamous cell carcinoma

WD

Well-differentiated

Background

The procedure of bronchoscopy for tissue sampling of lung peripheral masses has been traditionally carried out under fluoroscopic guidance, and the bronchial branch connected to the lesion has been classically estimated from the 2-dimensional lung computed tomography images taken prior to the bronchoscopic study [1]. However, the diagnostic yield of peripheral pulmonary lesions of less than 2 cm is very low (34%) when this conventional bronchoscopic method is used [1, 2, 3]. Radial endobronchial ultrasound (EBUS) and virtual bronchoscopic navigation system have been recently developed to improve the diagnostic yield of bronchoscopic techniques [4]. Indeed, recent studies have shown that the combined use of both techniques have dramatically increased (> 80%) the diagnostic yield of peripheral pulmonary lesions of less than 2 cm [2, 3]. Further, the diagnostic yield is even higher when the EBUS shows that the guide sheath is within the target lesion [5, 6]. Nonetheless, about 10% of pulmonary malignant lesions are not diagnosed despite being the guide sheath within the lesion [4]. The cause of this diagnostic failure is unclear. The aim of the present study was to describe retrospectively the clinical and histopathological characteristics of cases with diagnostic failure after EBUS with the guide sheath within the lesion.

Patients and methods

We retrospectively evaluated 347 patients with lung mass that underwent EBUS with guide sheath (EBUS-GS) at the Respiratory Center of Matsusaka Municipal Hospital between February 2015 and August 2016. Among 347 patients, 84 with benign lesion, 42 under follow-up, and 13 lost to follow-up were excluded from the study. Of the remaining 208 patients with lung mass, 26 with ground glass opacity and 13 with cavitation were excluded because it was difficult to confirm whether the guide sheath was within the lesion. During the EBUS-GS procedure performed in the remaining 169 patients, the guide sheath was within the target lesion in 132 cases, and among these, the histopathological study revealed malignant tumor in 122 cases and no malignant findings in 10 cases. Of these 10 cases, 2 were excluded because the final diagnosis of malignant tumor was possible only by clinical exclusion, and 8 underwent CT-guided needle biopsy or surgery for final diagnosis (Fig. 1). The histopathological diagnosis was malignant tumor in the 8 cases.
Fig. 1

Study selection procedure. The records of 347 patients with lung mass that underwent endobronchial ultrasound with guide sheath were evaluated and 130 cases with confirmed diagnosis and 8 cases with misdiagnosis were selected following the described criteria

The bronchoscopic procedure was performed as previously described [2]. A flexible bronchoscope was inserted after spraying 2% lidocaine hydrochloride on the pharyngeal mucosa and intravenous administration of 2 mg midazolam (SANDOZ Japan, Tokyo). Additional injection of midazolam was performed as required. The flexible bronchoscope used in the study was Olympus BF Type P260F (Olympus Medical Systems, Tokyo, Japan) with an external diameter of 4 mm and a channel diameter of 2 mm or Olympus BF Type P290 (Olympus Medical Systems, Tokyo, Japan) with an external diameter of 4.2 mm and a channel diameter of 2 mm. Insertion of the bronchoscope was assisted using a virtual bronchoscopic navigation system (Bf-NAVI; Cybernet Systems, Tokyo) as described [7] . The virtual bronchoscopic images were prepared using the computed tomography scan (TSX-101A/QA, Canon Medical Corporation, Tochigi, Otawara, Japan) data of the lungs as previously described [2]. A radial type EBUS probe of 1.4 mm (UM-S20-17S; Olympus Medical Systems, Tokyo) was introduced with a guide sheath (SG-200C; Olympus Medical Systems, Tokyo, Japan) through an endoscopic channel to visualize the lung lesions. Lung tissue samples were collected using forceps of 1.5 mm (FB-233D;Olympus Medical Systems, Tokyo, Japan) inserted into the guide sheath. After confirming visualization of the lesion, the EBUS probe was withdrawn. Brushing and washing were performed after completing the transbronchial biopsy.

Statistical analysis

Data are expressed as the median and range. The different between two groups with continuous variable was evaluated by student test and the difference in distribution by Fisher’s exact test. Statistical analyses were done using the Graph Prism version 8.0.1 (Graphpad Software, San Diego, CA). A p < 0.05 was considered as statistical significance.

Results

The backgrounds and characteristics of the patients are described in Table 1. There was no significant different in the age, sex distribution, tumor size, tumor location or in the number of times the virtual bronchoscopic navigation and rapid on site cytology was performed between misdiagnosis and correct diagnosis groups (Table 1). The lesions were grouped according to EBUS findings based on the classification proposed by Kurimoto et al. (Fig. 2) as follows: type IIa (hyperechoic dots and linear arcs without vessels; n = 1), type IIb (hyperechoic dots and linear arcs with patent vessels; n = 1), type IIIa (heterogeneous pattern with hyperechoic dots and short lines; n = 4) and IIIb (heterogeneous pattern without hyperechoic dots or short lines; n = 2) (Fig. 3) [8]. EBUS images showed heterogeneous patterns in cases 3 to 8, and there was significant difference in the EBUS pattern between both misdiagnosis and correct diagnosis groups (Table 1). There were 7 cases of primary lung cancer and 1 case of diffuse large B cell lymphoma but there was no significant difference in the final diagnosis between both misdiagnosis and correct diagnosis groups (Table 1).
Table 1

Characteristics of the patients

Variables

Cases with Misdiagnosis (n = 8)

Cases with correct diagnosis (n = 122)

P values

Median age (range)

73 (37–83)

74 (31–95)

0.76

Sex

  

0.27

 Male

4

84

 

 Female

4

38

 

Lesion size (mm, median range)

27.8 (15.0–48.0)

32.9 (5.5–81.0)

0.30

Location

  

0.058

 Central

0

11

 

 Intermediate

0

41

 

 Peripheral

8

70

 

Virtual bronchoscopic navigation, n(%)

8 (100%)

101 (82.8%)

0.35

Rapid on site cytology, n(%)

7 (87.5%)

89 (73.0%)

0.62

EBUS-image

  

0.04

 Type IIa

1

0

 

 Type IIb

1

4

 

 Type IIIa

4

57

 

 Type IIIb

2

57

 

 Unknown

0

4

 

Final diagnosis

  

0.32

 Primary lung cancer

7

117

 

 Malignant lymphoma

1

0

 

 Metastatic lung cancer

0

5

 
Fig. 2

Endobronchial ultrasound patterns in patients with misdiagnosis. The findings of the radial endobronchial ultrasound in 8 patients with misdiagnosis were grouped according to the classification reported by Kurimoto et al

Fig. 3

Computed tomography, endobronchial ultrasound and histopathological findings. Case 3 (a, b, c): tumor with fibrotic and granulomatous tissue. Case 4 (d, e, f): tumor with areas of fibrosis and necrosis. Case 5 (g, h, i): tissue sampling by transbronchial biopsy with tissue showing atypical cells. Case 6 (j, k, l): tumor with central necrosis. EBUS, endobronchial ultrasound; CTNB, CT-guided needle biopsy

Transbronchial biopsy was conducted three times in one case and over five times in the other cases. The diagnostic report of cytological smear disclosed negative findings in cases 1, 2, 3 and 6, presence of atypical cells in cases 5 and 7, and suspicious findings of cancer in cases 4 and 8. The report of histopathological examination of biopsy samples collected during bronchoscopy and EBUS-GS showed negative findings in cases 2 and 3, atypical cells in cases 1, 3, 5 and 7, and insufficient amount of tissue in cases 2 and 8. During the bronchoscopic procedure, case 1 had persistent cough, cases 2 and 8 showed irregular respiratory rhythms and cases 1, 2, 4 and 5 presented bronchial bifurcations with steep angles. Tissue sampling was not technically difficult in cases 3, 6 and 7 (Table 2).
Table 2

Factors associated with diagnostic failure

Cases

Bronchi

EBUS-i

Diagnosis

Technical factors

Pathological factors

1

LtB1 + 2a

IIa

WDadeno

Bending and difficult stabilization of guide sheath

Tumor with central necrosis

2

RtB6c

IIb

MD adeno

Bending and difficult stabilization of guide sheath

Homogeneous distribution of tumor cells

3

RtB1bii

IIIa

MD adeno

No difficulty

Heterogeneous tissue. Tumor cells in granulomatous and fibrotic tissue

4

LtB3ci

IIIa

MD adeno

Bending of guide sheath

Tumor central necrosis

5

RtB10bii

IIIa

WD adeno

Bending and difficult stabilization of guide sheath

Tumor cells with predominant distribution in central areas

6

RtB4aiβ

IIIb

PD SCC

No difficulty

Tumor with central necrosis

7

RtB2bii

IIIa

WDadeno

No difficulty

Tumor with central necrosis

8

LtB8biiα

IIIb

DLBCL

Difficult stabilization of guide sheath

Homogeneous distribution of tumor cells

EBUS-1 endobronchial ultrasound image, LtB left bronchus, RtB right bronchus, adeno adenocarcinoma, SCC squamous cell carcinoma, WD well-differentiated, MD moderately differentiated, PD poorly differentiated, DLBCL diffuse large B cell lymphoma

Histopathological examination of lung tissue specimens obtained by CT-guided needle biopsy or during surgery disclosed tumor central necrosis in cases 1, 4, 6 and 7, extensive areas of granulomatous and fibrotic tissue in case 3, lack of cancer cells in peripheral areas in case 5, and homogeneous distribution of cancer cells in cases 2 and 8 (Table 2; Fig. 3).

Discussion

The diagnostic yield of peripheral pulmonary lesions of less than 3 cm by bronchoscopy has substantially improved with the use of R-EBUS or virtual bronchoscopic navigation reaching 60 to 80% when both virtual bronchoscopic navigation and EBUS-GS are used in combination [4, 9]. The diagnostic yield is particularly high when the EBUS guide sheath is placed within the target lesion [4]. However, misdiagnosis has been reported even in cases with the guide sheath within the lesion but the cause remains unclear. Here, we reported 8 cases of diagnostic failure despite being the guide sheath within the tumor site. Six patients (cases 3, 4, 5, 6, 7, 8) from the misdiagnosis group showed heterogeneous EBUS images and the EBUS pattern was significantly different between both misdiagnosis and correct diagnosis groups. Further, six patients (cases 1, 3, 4, 5, 6, 7) of the misdiagnosis group also showed inhomogeneous distribution of malignant cells within the tumor. Therefore, heterogeneity in the EBUS findings and in the intra-tumoral distribution of malignant cells appears to be important factors leading to failure of diagnosis. Sampling of large-sized or multiple specimens may improve the diagnostic yield. Yamada et al reported diagnostic yield of 96.5% in cases with 5 biopsied specimens and 100% in cases with 10 specimens [6]. The use of large forceps or cryobiopsy may also be an alternative approach to collect large-sized specimens [10, 11].

We found here that technical difficulty is another important cause of misdiagnosis. Sample collection was difficult in 5 patients (cases 1, 2, 4, 5) because steep angle of the bronchial branch restrained complete bending of the bronchoscope tip. In these cases, the use of ultra-thin bronchoscope in combination with R-BUS may help to overcome technical difficulties and to improve diagnostic yield as reported by Oki et al [12]. We also experienced technical difficulty in one patient with persistent cough (case 1) and in 2 patients with irregular respiratory rhythm (cases 2, 8). We believe that insufficient sedation during the bronchoscopic procedure could have been the cause. In this regards, the guidelines of the British Thoracic Society recommends sedation with benzodiazepines or/and opioids to improve tolerance and facilitate diagnostic procedures during bronchoscopy [13]. Adequate sedation can prevent throat reflex and coughing and facilitate specimen sampling [13].

Conclusion

In brief, the results of this study showed that technical difficulties and/or heterogeneity of EBUS and histopathological findings of the tumor might lead to failure to diagnose lung malignant tumor even in cases using EBUS with the guide sheath within the tumor. The cause of diagnosis failure reported in this study may serve as important hints for developing countermeasures to improve the diagnostic yield in patients with lung peripheral malignant tumors.

Notes

Acknowledgements

Not applicable.

Authors’ contributions

YN, TK, OH, and ECG contributed in conceptualization and idea of the study. KI, YS, FW, TY, KN, CND-G and HF made contributions in the supervision and data analysis. TY, ECG, FA and CND-G edited the manuscript. OT and OH contributed in resources and in the interpretation of the data. All authors read and approved the final manuscript.

Funding

No grant was received for conducting this study.

Ethics approval and consent to participate

Informed consent was obtained from all patients before the diagnostic procedures. The Institutional Review Board of Matsusaka Municipal Hospital approved the study protocol (Approval No.180601–6-5; Date: 2018/6/14), and the study was performed following the Principles of Helsinki Declaration.

Consent for publication

Consent has been obtained for publication of images.

Competing interests

The authors declare that they have no competing interests.

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Copyright information

© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Authors and Affiliations

  • Yoichi Nishii
    • 1
  • Taro Yasuma
    • 2
  • Kentaro Ito
    • 1
  • Yuta Suzuki
    • 1
  • Fumiaki Watanabe
    • 1
  • Tetsu Kobayashi
    • 3
  • Kota Nishihama
    • 2
  • Corina N. D’Alessandro-Gabazza
    • 2
  • Hajime Fujimoto
    • 3
  • Esteban C. Gabazza
    • 2
    Email author
  • Fumihiro Asano
    • 4
  • Osamu Taguchi
    • 1
  • Osamu Hataji
    • 1
  1. 1.Respiratory Center, Matsusaka Municipal HospitalMatsusakaJapan
  2. 2.Department of ImmunologyMie University Faculty and Graduate School of MedicineTsu-cityJapan
  3. 3.Department of Pulmonary and Critical Care MedicineMie University Faculty and Graduate School of MedicineTsuJapan
  4. 4.Gifu Prefectural General Medical CenterGifuJapan

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