Prostaglandin F2α (PGF2α) production possibility and its receptors expression in the early- and late-cleaved preimplantation bovine embryos
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Prostaglandin F2α (PGF2α) is an important component for the physiology of female reproductive processes. In the literature, the data pertaining to the synthesis and action of PGF2α in early embryonic bovine development are limited. In our study, we used the bovine in vitro culture model based on the time of first cleavage to determine the mRNA expression and immunolocalization of PGF2α synthase and its receptor in bovine embryos from the 2-cell stage to the hatched blastocyst stage. We also evaluated PGF2α production at 2, 5 and 7 days of in vitro culture.
We found a significantly higher proportion of blastocysts obtained from the early-cleaved embryos than from the late-cleaved embryos (37.7% vs. 26.1% respectively, P < 0.05). The PGFS mRNA expression was significantly higher in the late-cleaved group than in the early-cleaved group at the 2-, 4- and 16-cell stages (P < 0.05). For PTGFR, we observed that within the late-cleaved group, the mRNA abundance was significantly higher in embryos at the 2- and 16-cell stages than in embryos at the 4- and 8-cell stages (P < 0.05). We observed that PTGFR mRNA expression was significantly higher in the 2- and 16-cell embryos in the late-cleaved group than that in the early-cleaved group embryos (P < 0.05). Among the blastocysts, the PGFS and PTGFR expression levels showed a trend towards higher mRNA expression in the late-cleaved group than in the early-cleaved group. Analysis of PGF2α production showed that within the early-cleaved group, the content of PGF2α in the in vitro culture medium was significantly higher on day 7 than it was on day 2 (P < 0.05).
The mRNA expression levels of PGF2α synthase and its receptor depend on the developmental stage and the embryo quality. Analyses of PGFS and PTGFR expression in bovine blastocysts and of PGF2α embryo production suggest that prostaglandin F2α can act in an autocrine and paracrine manner in bovine in vitro-produced preimplantation embryos. Moreover, the tendency of PTGFR and PGFS mRNA expression to be upregulated in embryos with low developmental potential can indicate a compensation mechanism related to high PGFS and PTGFR mRNA expression levels in low-quality embryos.
KeywordsEmbryo Bovine Prostaglandin F2α
Angiopoietin like 3
Analysis of variance
Assisted reproductive technology
Bovine serum albumin
Enzyme-linked immunosorbent assay
Polymerase chain reaction
Secreted seminal-vesicle Ly-6 protein 1
Prostaglandin F2 alpha (PGF2α) and other prostanoids are synthesized from arachidonic acid (AA), which is converted into unstable prostaglandin H2 (PGH2) by either prostaglandin endoperoxide synthase 1 or 2 (PTGS1 or PTGS2) [1, 2]; PGH2 is then metabolized into PGF2α by the 9,11-endoperoxide reductase (referred to as prostaglandin F2α synthase (PGFS); originating from the aldo-keto-reductase 1C (AKR1C) family) or through aldose reductase with 20α-hydroxysteroid dehydrogenase activity (AKR1B5, originating from the AKR1B5 family) [2, 3, 4]. The alternative pathway of PGF2α biosynthesis involves the reduction of PGE2 by 9-keto-prostaglandin-reductase (9 K-PGR), which is localized in the endometrium, placenta and corpus luteum of ruminants [5, 6, 7, 8]. PGF2α exerts its biological action through prostaglandin F2α receptor (PTGFR), which belongs to the group of G-protein-coupled receptors. Prostanoids belong to the group of biologically active lipids, which are well-known primary mediators of pathological conditions such as inflammation and cancer but are also essential for the physiology of female reproductive processes . In cows, increased uterine concentrations of PGF2α are correlated with high embryonic mortality and decreased pregnancy rates [10, 11]. Moreover, the addition of PGF2α to culture medium decreases the in vitro development of rabbit  and rat  embryos as well as the in vitro and in vivo development of bovine embryos . Additionally, Hockett et al.  showed that the administration of PGF2α exerts a negative influence on embryo quality and the ability to develop past the morula stage.
The developmental competence of oocytes is closely related to their quality and is the key limiting factor in the effective performance of assisted reproduction techniques in females. During in vitro embryo production, the selection of competent oocytes is crucial for successful fertilization, correct embryo development and subsequent high pregnancy rates. The knowledge of oocyte and embryo physiology during certain, defined steps of in vitro development may stimulate the further development of other techniques, such as the marker-assisted and genomic selection of preimplantation embryos or even benefit-assisted reproduction in human beings. There are several crucial steps during in vitro bovine embryo development. After fertilization, the zygote undergoes cleavage divisions into the 2-, 4-, 8- and 16-cell stages. Following this, at 32–64 cells, the blastomere overcomes polarization, which results in the compaction and formation of the morula stage. Compacting embryos encompass cellular events, including the development of Ca2+-dependent cell adhesion, the formation of gap junctions for cell communication between blastomeres, the initiation of cell contact-induced polarization, and the formation of cell-to-cell tight junctions, which can divide the plasma membranes of the outer blastomeres into apical and basolateral membrane domains. Thereafter, the complex process of cavitation results in blastocyst formation. During cavitation, trophoectoderm development occurs, which initiates the first contact with the uterus during implantation .
In the literature, we can find two types of models for the investigation of oocyte developmental competence. Lonergan et al.  and Patel et al.  described the bovine model based on the time of the first cleavage. According to their studies, good quality embryos cleave early, approximately 30 h post-fertilization, whereas embryos with low developmental competence cleave 6 h later. It has also been documented that early-cleaved bovine embryos develop to the blastocyst stage at a higher rate than their late-cleaved counterparts . The differences in the timing of the first cleavage result in a dissimilarity in transcriptome composition between the early- and late-cleaved embryos at the two-cell stage . However, the difference in the rigorous, time-dependent expression of the defined gene programme between the early- and late-cleaved embryos at all the previously mentioned crucial stages of their preimplantation development remains unclear. This time-dependent expression of the defined gene programme remains to be an area of particular interest for our research and includes the determination of the expression of enzymes, receptors and factors involved in PGF2α synthesis and action. Taking the above into consideration in our studies, we planned to describe the specific pathway of PGF2α synthesis and its receptor expression in early- and late-cleaved preimplantation bovine embryos during the crucial stages of their development in vitro. Moreover, we examined PGF2α production by early- and late-cleaved bovine embryos on days 2, 5 and 7 of in vitro culture.
Developmental rates of the early- and late-cleavage embryos at day 5 and day 7 of in vitro culture
Morula formation rates depending on the time of first cleavage
number of fertilized oocytes, n
fertilization rate, n
early-cleaved embryos (30 hpi), n
(48 hpi), n
morula formation rate, n
morulas from early-cleaved embryos at Day 5, n (%)
morulas from late-cleaved embryos at Day 5, n (%)
Blastocysts formation rates depending on the time of first cleavage
number of fertilized oocytes, n
fertilization rate, n
early-cleaved embryos (30 hpi), n
(48 hpi), n
blastocyst formation rate, n
blastocysts from early-cleaved embryos at Day 7, n (%)
blastocysts from late-cleaved embryos at Day 7, n (%)
The expression profile of PGFS and PTGFR in the preimplantation embryos
Immunolocalization of PGFS and PTGFR in bovine preimplantation embryos
The production of PGF2α by bovine preimplantation embryos
It is well known that embryos produced in vivo are of a higher quality in relation to their counterparts produced in vitro [19, 20]. Moreover, until the blastocyst stage, embryos have some autonomy. The oocytes aspirated from the ovarian follicles can be inseminated in vitro and cultured for approximately 7 days. During this time, they do not need exposure to the maternal reproductive tract to establish pregnancy. Similarly, the recipient’s oviduct does not need contact with the embryo to establish pregnancy during the embryo transfer procedure . Therefore, a detailed examination of the molecular mechanisms regulating preimplantation embryo development could increase the basic knowledge about the in vitro embryo production procedure and could identify factors involved in bovine reproduction; this will improve the procedure itself and will help to optimize reproductive efficiency for livestock production. To accomplish these goals, we should understand the mechanisms that control preimplantation embryo development and focus on the expression patterns and functions of genes that may be involved during these crucial steps. Prostaglandins are the key mediators of the female reproductive system, including ovulation, fertilization, pregnancy maintenance or parturition . In the present study, we investigated the expression of genes involved in the synthesis and action of PGF2α in two different groups of in vitro cultured bovine embryos at different developmental stages based on the time of their first cleavage. The obtained results proved that in the bovine preimplantation embryos, the mRNA expression of PGFS and PTGFR changed substantially according to the developmental stage of the preimplantation embryo as well as its quality. PGFS mRNA expression has been detected previously in bovine embryos but only at day 7 of in vitro culture . To the best of our knowledge, our report is the first to demonstrate the exact transcript expression of PGFS and PTGFR in bovine embryos from the 2-cell stage to the blastocyst stage. In our research, we observed that in the early stages of embryo development (2-, 4-, and 16-cells), the PGFS mRNA expression was higher in embryos originating from late-cleaved zygotes than from early-cleaved zygotes. This result could indicate that the PGF2α autocrine regulatory pathway exists, which is highly active in late-cleaved embryos. Hatamani et al.  demonstrated that during the first embryonic cleavage, major zygotic genome activation occurs. They observed that at the 2-cell and 4-cell stages, important processes are involved in the preparation of basic cellular mechanisms. It was demonstrated that this is the time when embryo development depends on maternal transcription . The literature data show a few models to investigate the oocyte and embryo quality . The model proposed by Lonergan et al.  proves that embryos that cleave 48 hpi reflect lower developmental competence than embryos that undergo the first cleavage after 30 hpi. Pers-Kemczyc et al.  used this model to investigate chromosome aberrations and the speed of development of early- and late-cleaved in vitro-produced bovine embryos. The authors detected that early-cleaved embryos were less often chromosomally abnormal and underwent divisions with a higher speed than late-cleaved embryos. They also documented that late-cleaved embryos were more often arrested at the 8–16-cell stage, the time of embryo development after which major gene activation (MGA) takes place, than early-cleaved embroys [27, 28]. MGA occurs between the fourth and fifth cell cycles and initializes biological and morphological alterations in preimplantation embryo development [23, 24]. Interestingly, in our study between the 8- and 16-cell stage embryos, we noticed the increased expression of PGFS in the late-cleaved embryos but noticed a decrease in the early-cleaved group. During the early stages of development (2- and 4-cells), the PGFS mRNA expression is under the control of maternal transcription. In the 8-cell stage embryo, when the maternal-to-zygotic genome transition (MZT) occurs, we observed an augmentation of PGFS mRNA expression in the early-cleaved embryos and a decline in the late-cleaved embryos. Finally, in the 16-cell embryo, we documented a decrease in the early-cleaved group and an increase in the late-cleaved group; at this time, the genomic control transitioned to embryonic control. This means that the differences in the PGFS mRNA expression within the early- and late-cleaved groups of embryos depend on the MZT. The next important step during early embryo development is compaction, which is initiated in cows at the 16–32-cell stage and continues until morula formation . During compaction, important processes encompass the cells within cell adhesion molecules and determine trophoblast differentiation and blastocyst morphogenesis [16, 29]. It was documented that incorrect cell-to-cell interactions manifested by the lack of E-cadherin or gap junction disruption may result in a decrease in blastocyst formation, developmental disturbances and embryonic death [30, 31, 32]. In our results, we observed that at the time of cavitation, the PGFS mRNA expression was downregulated from the 8- or 16-cell embryos to the morula stage in the early- and late-cleaved embryos, respectively. These changes in the PGFS mRNA expression, which occur during compaction, suggest some relevance of PGF2α synthesis during the time of blastomere polarization and the establishment of the first cell-to-cell contact in embryos, and they lead to the formation of a compact morula. After this developmental step, we did not observe differences in PGFS mRNA expression among all grades of the morula stage up to the blastocyst stage when cavitation takes place. Among the blastocyst stage, we can distinguish early, developed, expanded and hatched blastocysts. In cows, the PGFS mRNA expression was reported by Torres et al.  in day 7 pre-hatched blastocysts. Our research shows how the mRNA expression differs between the particular stage and the quality of the blastocysts (according to IETS) obtained from the early- and late-cleaved groups of embryos. At the early blastocyst stage (grade A), we observed that the PGFS mRNA expression was more abundant in the late-cleaved group than in the early-cleaved group. Similarly, we observed high PGFS gene expression in the developed (grade C) and hatched blastocysts obtained from the early- and late-cleaved zygotes. Moreover, we depicted that among the expanded blastocysts from the early-cleaved group, the PGFS mRNA expression was upregulated in grade C blastocysts in relation to their counterparts from grades A and B. Moreover, among the early-cleaved group of blastocysts, the percentage share was the highest for blastocyst 7A but was the lowest for 7C. Our results indicate that the PGFS mRNA expression is commonly high in blastocysts belonging to the late-cleaved group or grade C blastocysts, which are known to reflect low-quality embryos. This suggests that the potential PGF2α autocrine regulatory pathway is more intense in low-quality blastocysts.
In our research, we also analysed the expression pattern of PTGFR in bovine preimplantation embryos. The PGF2α receptor was previously detected in the bovine uterus , the corpus luteum  and blastocysts . The PGF2α receptor belongs to the G-protein coupled receptors whose activation stimulates the release of the intracellular Ca2+ concentration, which consequently activates protein kinase C (PKC) . Gao et al.  demonstrated that in the bovine endometrium, PCK-dependent PTGFR activation stimulated the expression of factors involved in cell proliferation, migration, differentiation, angiogenesis and tissue repair (PTGS-2, VEGF, CTGF, TGFB-1, IL-8). In the porcine endometrium, the increased expression of the PGF2α receptor activated the expression of genes that participate in tissue remodelling and angiogenesis at the time of implantation . It was shown that activation of the PGF2α receptor can result in the disruption of gap and tight junctions, the perturbation of cell-to-cell communication and the induction of apoptosis [39, 40, 41]. The above data indicate that the role of PGF2α in important intracellular processes is necessary for proper implantation and cell survival; in addition, these data indicate that PGF2 can act in an autocrine and paracrine manner in bovine preimplantation embryo development.
We detected that PTGFR expression was significantly higher in the late-cleaved group of 2- and 16-cell stage embryos than in the early-cleaved group. Moreover, among the late-cleaved group, we detected the downregulation of PTGFR transcript abundance between the 2- and 4-cell stages; we also detected upregulation between the 8-cell and 16-cell stages of development. These results suggest that after embryonic genome activation, in the late-cleaved group, these changes could be related to major zygotic genome activation and MGA processes, as mentioned above. Analysis of the morula stage of development showed that the PTGFR mRNA expression in the late-cleaved group was significantly higher in grade C morulas than in grade A and B morulas. Within grade C morulas the transcript abundance for PTGFR was higher in the late-cleaved group than in the early-cleaved group. Additional analysis of PTGFR expression between the 2- and 16-cell embryos and the morula stage indicated that in late-cleaved embryos, the mRNA transcript abundance was significantly higher in grade C morula compared to that in all remaining stages. This suggests that in low-quality embryos, the processes that take place during MGA and compaction can be regulated by PGF2α in a paracrine manner. Analysis of PTGFR expression among the blastocyst stage showed the stable expression of this gene in the early-cleaved group and the upregulation from grade A to grade C among the early and expanded blastocysts belonging to the late-cleaved group of embryos. Furthermore, during the analysis of the early and developed grade C embryos, which reflect a low quality, we observed a high expression of PTGFR in the late-cleaved group of embryos. Our results suggest that the low quality of embryos reflects a high level of the PTGFR transcript. In the literature, we found a widely described negative influence of PGF2α on embryo quality and development [10, 11, 15]. Kim et al.  observed that PGF2α addition to the culture medium decreased the mRNA expression of PTGS2, the correct level of which is correlated with proper blastocyst implantation, trophoblast proliferation and embryo-maternal interaction. They suggested that the downregulation of PTGS2 synthesis can reduce the embryo quality. This research group indicated that PGF2α suppressed SSLP1 expression, for which the increased expression was positively correlated with blastocyst hatching. Moreover, they described that CASP3, which is considered one of the main apoptotic factors, was upregulated in bovine blastocysts after stimulation of PGF2α. According to the above data (which show that the addition of PGF2α to the culture medium decreased the number of blastocysts and pregnancies) as well as our results (which indicate a high PTGFR expression in low-quality embryos), we can assume that the level of PTGFR expression in the bovine embryos can reflect their developmental competences. The trend of high PTGFR transcript abundance in low-quality embryos in connection with the high PGF2α synthesis possibility (expressed by PGFS mRNA expression) indicate that in vitro-produced bovine embryos might be the target and the source of PGF2α.
The measurement of the PGF2α concentration in the culture medium at days 2, 5 and 7 showed that the examined bovine preimplantation embryos from both the early- and late-cleaved groups were able to produce PGF2α in different patterns. Our results showed that PGF2α production changes during in vitro culture and depends on the quality of the embryos. PGF2α produced by in vitro cultured embryos has been reported previously in rabbits  sheep , mares , sows , women  and cows [35, 48]. The expression of the receptor for PGF2α was reported in the bovine endometrium and myometrium . Different studies indicate that PGF2α secretion as well as PGFS and PTGFR expression are positively correlated with endometrium growth during the oestrous cycle and early pregnancy . Kaczyński et al.  demonstrated that PGF2α can regulate the expression of genes involved in the establishment of pregnancy and angiogenesis in the endometrium during early pregnancy in sows. In the ovine uterus, PGF2α upregulated ANGPTL3 gene expression, which is associated with angiogenesis and consequently increased uterine blood flow during early pregnancy . Zhang et al.  demonstrated the influence of PTGFR agonists on cell proliferation and growth factors in the bovine endometrium during the oestrous cycle and early pregnancy. Moreover, activation of the PGF2α receptor may take part in the regulation of genes involved in endometrial repair during the oestrous cycle in the cow . Our results showed that PGF2α production in the culture medium increased from day 2 to day 7 of in vitro culture in the early-cleaved group of embryos and was stable in the late-cleaved group of embryos. The obtained data prove that PGF2α production is high when the embryo reaches the blastocyst stage and implantation occurs. This is consistent with the observation that PGF2α can impose a paracrine effect on the endometrial tissues and, in this way, may influence the processes involved in implantation, uterine receptivity and the establishment of pregnancy . Moreover, we observed significantly higher concentrations of PGF2α in the day 7 culture medium obtained from the early-cleaved group than those from the late-cleaved group. Pers-Kamczyc et al.  noticed that early-cleaved embryos reflect high quality. These results suggest that PGF2α production increased during in vitro culture in the early-cleaved group of embryos. PGF2α is produced more abundantly at day 7 of IVP by the early-cleaved group than by the late-cleaved group. The above data suggest that this prostanoid could reflect the quality of bovine embryos produced in vitro.
In summary, we described the mRNA expression of genes involved in PGF2α synthesis and the development of bovine preimplantation embryos from the 2-cell to the hatched blastocyst stage obtained from early- and late-cleaved zygotes. Additionally, the presence of PGFS and PTGFR in the analysed embryos was confirmed by immunofluorescence staining. The mRNA expression of PGF2α synthase and its receptor depends on the developmental stage and embryo quality. Moreover, analyses of PGFS and PTGFR expression in bovine blastocysts and of PGF2α embryo production suggest that prostaglandin F2α can act in an auto- and paracrine manner in bovine in vitro-produced preimplantation embryos. Our results show the tendency of PTGFR and PGFS mRNA expression to be upregulated in embryos with low developmental potential, which can indicate that some compensation mechanism related to high PGFS and PTGFR mRNA expression exists among late-cleaved, low-quality embryos. Moreover, late-cleaved embryos reflect high cleavage rates, and during in vitro culture, they develop to the blastocyst stage, which can indicate the possibility of using them in ART techniques just as early-cleaved embryos are used. However, the usefulness of these embryos and the implantation and pregnancy rates need to be confirmed with future research. Our data confirmed the utility of the model for examining the quality of embryos based on the time of first cleavage. The dynamic changes in mRNA expression of PGF2α synthase and its receptor in the analysed group of embryos could indicate that these factors can alter the embryonic development of in vitro-produced bovine preimplantation embryos.
Cumulus-oocyte complex collection
The described animal procedures were carried out after providing the approval from the Local Animal Care and Use Committee in Olsztyn, Poland (Agreement No. 76/2014/DTN). The material for all experiments were the ovaries obtained from non-pregnant Holstein cows with normal cycles from a local slaughterhouse (“Warmia”, Biskupiec, Poland). The ovaries were collected in the vial containing sterile 0,9% saline solution at 37 °C within 20 min of the slaughtered and were subjected to further processing in the laboratory after 40 min.
Aspiration of follicular fluid from less than 5 mm diameter subordinate ovarian follicles gave the cumulus-oocyte complexes (COCs). The oocytes were separated under the assessment by stereomicroscope and only COCs surrounded by cumulous cells homogenously by at least three layers of cumulus cells and without signs of ooplasm degradation were taken for the future studies. The COCs were washer two times in wash medium (M199; #M5017, Sigma Aldrich, Germany) supplemented with 20 mM HEPES (#H4034 Sigma Aldrich, Germany), 25 mM sodium bicarbonate (#S4019 Sigma Aldrich, Germany), 0,4% bovine serum albumin (#A9418 BSA; Sigma Aldrich, Germany) and 40 μg/ml gentamicin (#G1272, Sigma, Aldrich, Germany) and subsequently one time in maturation medium.
In vitro embryo production
After segregation, groups of 50 COCs were placed in four-well plates (#144444, Thermo Fisher Scientific, USA) containing 400 μl of maturation medium (TCM 199 Maturation Medium (19,990/0010, Minitube, Germany) suplemented with 0,02 IU/ml pregnant mare’s serum gonadotropin (PMSG, #G4527, Sigma Aldrich, Germany), 0,01 IU/ml human chorionic gonadotropin (hCG, #C0684, Sigma Aldrich, Germany) and 5% foetal bovine serum (FBS, #12106C, Sigma Aldrich, Germany) overlaid with 400 μl of culture oil (#M5310, Sigma Aldrich, Germany). The maturation was conducted for 24 h in humidified air atmosphere at 38.5 °C in 5% CO2. Thereafter, COCs were washed and fertilized in vitro in fertilization medium (TL fertilization medium 19,990/0030, Minitube, Germany), containing 10 μg/ml heparin (#H3393, Sigma Aldrich, Germany), 20 mM sodium pyruvate (#P3662, Sigma Aldrich, Germany) and 0,6% BSA using frozen semen obtained from the same bull. The semen was thawed and capacitated through the swim-up procedure in capacitation medium (TL sperm capacitation medium, 19,990/0020, Minitube, Germany) supplemented with 1 mM sodium pyruvate, 0,6% BSA and 0,1 mg/ml gentamicin for 1 h under the same conditions (38.5 °C, 5% CO2 humidified air atmosphere). After recovering the motile sperm, which was located in the upper two-thirds of the medium, it was centrifuged at 200×g for 10 min. For the fertilization only the sperm was used, which diluted in fertilization medium. The fertilization was conducted by co-incubation of 25 COCs groups with semen (concentration 106/ml) in four-well dishes containing 400 μl of fertilization medium overlied by 400 μl of mineral oil. The day of semen capacitation and in vitro insemination was regarded as day 0. After 30 h of incubation at 38.5 °C in a 5% CO2 humidified air atmosphere, the embryos were secluded from the cumulus cells by vortexing and followed by washing three times in the wash medium. The early- and late-cleaving groups of embryos were separated according to Patel et al. . The embryos cleaved 30 h after fertilization provided the group of early-cleaving embryos (n = 5; each pool consisted of five embryos for RNA extraction). The group of the late-cleaving embryos (n = 5; each pool consisted of five embryos for RNA extraction) were obtained from the same wells 36 h after fertilization. The early- and late-cleaving embryos were cultured in groups of 50 embryos in four-well dishes containing 400 μl culture medium (SOF; synthetic oviduct fluid medium; 19,990/0040, Minitube, Germany) supplemented with amino acids (10 μl/ml BME (#B6766, Sigma Aldrich, Germany) and 20 μl/ml MEM (#M7145, Sigma Aldrich, Germany), 3,3 mM sodium pyruvate and 5% FBS overlied by 400 μl of mineral oil. The embryos were incubated at 38.5 °C in an atmosphere of 5% CO2, 5% O2, and 95% N2 with high humidity up to day 8 of in vitro culture.
The developmental rates and quality for embryos from the early- and late-cleaving zygotes were provided by morphological examination on a stereo- or inverted microscope at 20, 50 or 100x magnification according to the International Embryo Transfer Society (IETS).
Sample collection for RNA isolation and reverse transcription
The pools of 5 embryos from early- and late-cleaving group at different stages of in vitro development suspended in the extraction buffer were used for RNA isolation according to the manufacturer’s instructions (#KIT0204, Arcturus PicoPure RNA Isolation Kit, Applied Biosystems, USA). For remove of genomic DNA contamination the RNase-Free DNase Set was used (#79254, Qiagen, Germany). After isolation, the samples were stored at − 80 °C. Reverse transcription (RT) was assessed using oligo (dT)12–18 primers (#18418–012, Invitrogen, USA) by Super Script III reverse transcriptase (#18080–044, Invitrogen, USA). The total volume of RT reaction stand at 20 μl. The reverse transcription reactions were performed by incubation at 65 °C for 5 min and 42 °C for 60 min, followed by 15 min step of denaturation at 70 °C. For degradation of the RNA strand of RNA-DNA hybrids we conducted by adding RNase H (#18021–071, Invitrogen, USA) to the samples (37 °C for 20 min). After RT, obtained products were stored at − 20 °C until real-time PCR amplification.
Quantitative real-time PCR
Primers used for Real-time PCR
GenBank accession No.
Primer sequence 5′ to 3′
The immunofluorescence staining was conducted for selected embryos. At the first step of staining the embryos were fixed in 4% paraformaldehyde for 1 h at 4 °C, washed in PBS, and permeabilized with 0.5% Triton X-100 for 10 min at room temperature. Permeabilized embryos were washed in PBS and incubated for 1 h in the blocking solution (Novocastra Protein Block, ref. RE7102, Novocastra Laboratories Ltd., Newcastle upon Tyne, United Kingdom). Afterwards, the embryos we incubated with the antibodies diluted 1:200 in blocking solution: rabbit polyclonal anti-PGFS (ab84327, Abcam, United Kingdom) and anti-PTGFR (#101802, Cayman Chemical), USA whose specificity was confirmed previously by WB and IHC. Following overnight incubation (4 °C) embryos were washed in PBS and incubated for 1 h at room temperature with Alexa Fluor 488 donkey anti-rabbit IgG antibody (concentration 1:1000 in blocking solution, A-21206, Invitrogen, Life Technologies, Foster City, CA, USA). For the negative control embryos were incubated only with the secondary antibodies. After final washing in PBS, the embryos were mounted in Vectashield with DAPI (#H-1200, Vector Laboratories, USA). The analysis of protein immunostaining was conducted under an Olympus Fluoview FV10i confocal laser scanning microscope.
The PGF2α concentration in the culture medium was determined. The samples were collected on days 2, 5 and 7 post-insemination with the time of first cleavage taken into account. The concentration of PGF2α in the conditioned media was measured using the PGF2α high sensitivity ELISA kit (#ADI-931-069, Enzo Life Sciences, USA) according to the manufacturer’s instructions. The standard curve for PGF2α ranged from 19.5 to 50,000 pg/ml. The samples were measured in duplicate.
The obtained data were analysed using the statistical software GraphPad PRISM 6.05 (GraphPad Software, Inc., La Jolla, CA, USA). All numerical data are presented as the mean ± SEM. The differences in the mRNA expression and the PGF2α concentration were analysed by two-way ANOVA, followed by Bonferroni’s multiple comparison test. The cleavage and blastocyst rates were analysed by a Chi-square test with Yate’s correction. The differences were considered statistically significant at the 95% confidence level (< 0.05).
The authors are indebted to Olympus Polska Sp. z o.o. for the possibility to use microscope Olympus Fluoview FV10i for capture the bovine embryos as well as to the owner of the slaughterhouse (Zaklady Miesne “Warmia”, Biskupiec, Poland), for facilitating material collection.
KG and IWP designed the study. KG performed the experiments and analyzed the data. DB, ES, IKZ and JSCh participated in execution of the experiments and the collection of data. KG wrote the paper. All authors read and approved the final manuscript.
This research was supported by Grants-in-Aid for Scientific Research from the Polish National Science Centre: NCN 2015/17/B/NZ9/011688. The Polish National Science Centre was the founder of the research and did not participate in the design of the study and collection, analysis, and interpretation of data as well as writing the manuscript.
Ethics approval and consent to participate
All animal procedures were approved by the Local Animal Care and Use Committee in Olsztyn, Poland (Agreement No. 76/2014/DTN).
Consent for publication
The authors declare that they have no competing interests.
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