Early onset of graft glomerulopathy in a patient with post-transplant diabetes mellitus after renal transplantation: a case report
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Post-transplant diabetes mellitus (PTDM) is an emerging problem in kidney transplantation, representing an important risk factor for kidney function loss. Diabetic nephropathy (DN) occurrence in transplanted kidneys is poorly investigated. Current knowledge describes DN recurrence in graft 5.9 years from kidney transplantation however there is little data about PTDM and DN.
Here, we report a clinical case peculiar for an early appearance of advanced glomerular diabetic lesions, after kidney transplantation.
A 45-year-old Caucasian male affected by autosomal polycystic kidney disease was transplanted with a cadaveric-kidney-donor from 58-year-old male. Induction immunosuppressive therapy included basiliximab and steroids while the maintenance treatment included, tacrolimus, mofetil micophenolate and methylprednisolone.
One month after transplantation the patient developed diabetes requiring treatment with repaglinide quickly replaced with insulin to obtain an acceptable glycemic control (HbA1c 52 mmol/mol). Glycosuria was detected persistently during the first six months after transplantation. To achieve further improvement in glycemic control, a shift from tacrolimus to cyclosporine (CyA) was made and steroids were rapidly tapered and stopped. To minimize calcineurin inhibitors toxicity, which was revealed in the 1-year-protocol-biopsy, everolimus was introduced thereby lowering CyA through levels. Moderate hypertension was well controlled with doxazosin. Thirty months after transplantation a second graft biopsy was performed owing to renal function decline and microalbuminuria appearance. Histological analysis surprisingly showed mesangiolysis and microaneurysms; glomerular sclero-hyalinosis and basal membrane thickness and typical nodular glomerulosclerosis. C4d staining was negative and no evidence of immune deposits were detected. Donor Specific Antibodies, serum C3 and C4 levels and autoimmunity tests were negative. Retrospective analysis on donor history didn’t show diabetes or insulin resistance and no diabetic lesions were found in kidney pre-implant biopsy.
In our knowledge, this is the first report describing a very early onset of advanced diabetic glomerular lesions in a graft biopsy after PTDM. We hypothesize that additional factors such as everolimus and hypertension, may have contribute to kidney damage.
KeywordsPTDM Kidney transplantation; diabetic nephropathy Mesangiolysis CNI Microalbuminuria HbA1c mTOR inhibitors
Angiotensin-Converting Enzyme Inhibitors
American Diabetes Association
Angiotensin II Receptor Blockers
Body Mass Index
End Stage Renal Disease
Human Leukocyte Antigen
mammalian Target Of Rapamycin
Post-Transplant Diabetes Mellitus
Renal Resistive Index
Post-transplant diabetes mellitus (PTDM) is a metabolic complication following renal transplant whose incidence ranges between 4 and 25%. Often the rapid onset and the accelerated course of diabetic nephropathy (DN) in post transplantation, if not recognized promptly, can have serious consequences. So a multidisciplinary approach in post transplantation and the endocrinologist’s role in these patients are crucial. PTDM exhibits similar complications to those seen in patients with type II diabetes, but with an accelerated rate, which can worsen the outcomes of transplant including graft failure and death .
Areas of mesangiolysis with glomerular capillary microaneurysm are seen in native kidneys after several years of diabetes and these are the hallmark of advanced diabetic DN.
We report an interesting case of advanced diabetic lesions such as mesangiolysis, microaneurysm and nodular glomerulosclerosis observed in a kidney biopsied after only 30 months from transplantation.
To the best of our knowledge, this is the first case reporting an early occurrence of advanced diabetic lesions in PTDM after kidney transplant.
One month following cadaveric kidney transplantation a 45-year-old Caucasian man, under tacrolimus, micophenolate mofetil (MMF) and steroids immunosuppression developed PTDM. Thirty months after transplantation histological graft changes characterized by mesangial sclerosis, mesangiolysis with glomerular capillary ectasia and microaneurysms appeared (Additional file 1). Diagnostic criteria for PDTM were consistent with current American Diabetes Association (ADA) clinical practice recommendation to diagnose diabetes in the general population .
Discussion and conclusions
Post transplant diabetes is an emerging problem in solid organ transplantation. Most of the anti-rejection drugs used such as glucocorticoids, tacrolimus and sirolimus are burdened by a diabetogenic effect. The prevailing factors causing diabetes by steroids seem to be the aggravation of insulin resistance, defects of insulin secretion and beta-cell apoptosis, while a decline in beta-cell survival and an impairment of insulin secretion are described as mechanism operated by CNI and sirolimus .
Nevertheless, the wide use of these drugs, especially tacrolimus, is justified by their powerful immunosuppressive effect.
While a great prevalence of PTDM is observed, not all the diabetic patients experience DN. Additionally, DN is not fully explained by poor glycemic control. Albeit the pathways that lead to DN in the native kidney have been extensively explored, there is still a paucity of data on DN after kidney transplantation .
Intrinsic susceptibility to diabetes-related damage in allograft kidneys could be the answer to several factors. Genetic predisposition is an important one; certain human leukocyte antigen (HLA) phenotypes, notably A30, B8, B15, B27, B42, and DR4, are associated with DN, but in our case neither donor nor recipient had HLA-predisposing phenotypes. The only familial factor risk was that the recipient’s mother became diabetic in old age.
Obesity is a known relevant contributor of glomerulosclerosis, but our patient always showed normal BMI values.
The reduced number of glomeruli per kidney has been suggested as another possible individual risk factor for the rapid progression of DN. Even though the donor and recipient were similar in size, the donor was 13 years older than the recipient, this fact could explain a potential reduced number of undamaged glomeruli. Broad evidence supports the association between low birth weight, premature childbirth and lower nephron number; unfortunately there is no such data available for our donor, consequently we cannot with confidence evaluate the equity of glomeruli .
In addition diabetes leads to severe impairment of renal vasomotor capacity, leaving the glomeruli unprotected by systemic high-pressure levels . The resulting glomerular over-perfusion may justify the mesangiolysis and glomerular microaneurysm formation, observed in the patient. Experimental models confirm our evidence, showing a link between hypertension, mesangiolysis, endothelial cell loss and glomerular capillary dilatation .
Although published clinical evidences show the benefits of ACE-I and/or ARBs on DN progression in native kidneys, limited information exists regarding the benefit of therapeutic interventions, e.g. ACE-I or ARBs, on reducing proteinuria or microalbuminuria after transplantation or PTDM nephropathy and kidney graft survival [5, 6].
In the transplanted patients we need to consider other probable mesangiolysis triggers like acute vascular CNI toxicity, immune-mediated glomerulonephritis and antibody-mediated rejection. However, serological and histological tests performed in the patient, ruled out those causes. The advanced stage of glomerulosclerosis can be justified even in the absence of immune deposits as demonstrated in previous published cases .
The anti-proliferative effect of mTOR inhibitors may negatively impact the glomerular repair, lead by growth factors, after mesangiolysis as was observed in the animal models [8, 9]; however in humans it is still an open issue. For the lack of evidence against everolimus, we didn’t plan the drug withdrawal in our patient.
The rapid recurrences of DN after kidney transplantation has been published, but in all cases the patients were already diabetic . The detection of such serious and advanced lesions after only 30 months from PDTM, as described in this case report, is a novelty.
Interestingly, mesangiolysis occurred very early after PTDM even in the presence of controlled glycemia. An HbA1c level < 7% (54 mmol/mol) is a reasonable goal for many patients but it’s not enough in those where hypertension is associated. Patients with PTDM should be strictly followed for microalbuminuria, glycemic and pressure control to minimize the risk of DN onset. Renal biopsy should drive the therapeutic changes and provide new insights into disease knowledge. In the presence of DN it would be recommended to correct all diabetes modifiable risk factors including a controlled lipid profile, no smoking and a healthy life-style. A careful evaluation of immunosuppressive therapy using non-diabetogenic drugs is important.
In conclusion, reducing the dose or discontinuing CNI and steroids could potentially limit the damage to beta cell, although the clinical evidence is poor. However it is difficult to weigh the risks related to rejection and/or PTDM in individual patients to choose the best immunosuppressive medication regimen.
Future perspective will evaluate whether belatacept, a new immunosuppressive drug with higher affinity to podocyte B7–1 antigen, expressed on hyperglycemia damaged podocytes, will be effective in preventing the development of DN after kidney transplantation.
Availability of data and material
The datasets used and/or analysed during the current study available from the corresponding author on reasonable request.
No funding was obtained for this study.
GM conceived the study, analysed and interpreted the data and drafted the manuscript. SV revised the manuscript critically for important intellectual content. PEF followed the patient while hospitalized and contributed to data collection. AA contributed to acquisition of literature data collection and analysis. RT revised the manuscript and approved the final version. All authors read and approved the final manuscript.
Ethics approval and consent to participate
The named local ethics committee did not require that formal ethics approval.
Consent for publication
Patient gave a written consent for their personal or clinical details along with any identifying images to be published in this study.
The authors declare no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
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