Advertisement

Genetics Selection Evolution

, 35:685 | Cite as

Chromosomal rearrangements in cattle and pigs revealed by chromosome microdissection and chromosome painting

  • Alain Pinton
  • Alain DucosEmail author
  • Martine Yerle
Open Access
Research

Abstract

A pericentric inversion of chromosome 4 in a boar, as well as a case of (2q-;5p+) translocation mosaicism in a bull were analysed by chromosome painting using probes generated by conventional microdissection. For the porcine inversion, probes specific for p arms and q arms were produced and hybridised simultaneously on metaphases of a heterozygote carrier. In the case of the bovine translocation, two whole chromosome probes (chromosome 5, and derived chromosome 5) were elaborated and hybridised independently on chromosomal preparations of the bull who was a carrier of the mosaic translocation. The impossibility of differentiating chromosomes 2 and der(2) from other chromosomes of the metaphases did not allow the production of painting probes for these chromosomes. For all experiments, the quality of painting was comparable to that usually observed with probes obtained from flow-sorted chromosomes. The results obtained allowed confirmation of the interpretations proposed with G-banding karyotype analyses. In the bovine case, however, the reciprocity of the translocation could not be proven. The results presented in this paper show the usefulness of the microdissection technique for characterising chromosomal rearrangements in species for which commercial probes are not available. They also confirmed that the main limiting factor of the technique is the quality of the chromosomal preparations, which does not allow the identification of target chromosomes or chromosome fragments in all cases.

Keywords

chromosome rearrangement microdissection cattle pig chromosome painting 

(To access the full article, please see PDF)

Copyright information

© INRA, EDP Sciences 2003

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Authors and Affiliations

  1. 1.UMR INRA-ENVT cytogénétique des populations animalesÉcole nationale vétérinaire de ToulouseToulouse Cedex 3France
  2. 2.Laboratoire de génétique cellulaireInstitut national de la recherche agronomiqueCastanet-Tolosan CedexFrance

Personalised recommendations