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A specific pattern of splicing for the horse α S1-Casein mRNA and partial genomic characterization of the relevant locus

  • Dragan Milenkovic
  • Patrice Martin
  • Gérard Guérin
  • Christine LerouxEmail author
Open Access
Research

Abstract

Mares' milk has a composition very different from that of cows' milk. It is much more similar to human milk, in particular in its casein fraction. This study reports on the sequence of a 994 bp amplified fragment corresponding to a horse α S1-Casein (αS1-Cn) cDNA and its comparison with its caprine, pig, rabbit and human counterparts. The alignment of these sequences revealed a specific pattern of splicing for this horse primary transcript. As in humans, exons 3', 6' and 13' are present whereas exons 5, 13 and 14 are absent in this equine mRNA sequence. BAC clones, screened from a horse BAC library, containing the αS1-Cn gene allowed the mapping of its locus by FISH on equine chromosome 3q22.2-q22.3 which is in agreement with the Zoo-FISH results. Genomic analysis of the αS1-Cn gene showed that the region from the second exon to the last exon is scattered within a nucleotide stretch nearly 15-kb in length which is quite similar in size to its ruminant and rabbit counterparts. The region between αS1- and β-Cn genes, suspected to contain cis-acting elements involved in the expression of all clustered casein genes, is similar in size (ca. 15-kb) to the caprine and mouse intergenic region.

Keywords

horse αS1-Casein cDNA localisation 

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Copyright information

© INRA, EDP Sciences 2002

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Authors and Affiliations

  • Dragan Milenkovic
    • 1
    • 2
  • Patrice Martin
    • 1
    • 2
  • Gérard Guérin
    • 1
    • 2
  • Christine Leroux
    • 1
    • 2
    Email author
  1. 1.Institut national de la recherche agronomique, Département de génétique animale, Laboratoire de génétique biochimique et de cytogénétique, Centre de recherches de JouyJouy-en-Josas CedexFrance
  2. 2.Institut national de la recherche agronomique, Département d'élevage et de nutrition des animaux, unité de recherches sur les herbivores, Centre de recherches de Theixsaint-genès-ChampanelleFrance

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