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Test for positional candidate genes for body composition on pig chromosome 6

  • Óvilo CristinaEmail author
  • Angels Oliver
  • José Luis Noguera
  • Alex Clop
  • Carmen Barragán
  • Luis Varona
  • Carmen Rodríguez
  • Miguel Toro
  • Armand Sánchez
  • Miguel Pérez-Enciso
  • Luis Silió
Open Access
Research

Abstract

One QTL affecting backfat thickness (BF), intramuscular fat content (IMF) and eye muscle area (MA) was previously localized on porcine chromosome 6 in an F2 cross between Iberian and Landrace pigs. This work was done to study the effect of two positional candidate genes on these traits: H-FABP and LEPR genes. The QTL mapping analysis was repeated with a regression method using genotypes for seven microsatellites and two PCR-RFLPs in the H-FABP and LEPR genes. H-FABP and LEPR genes were located at 85.4 and 107 cM respectively, by linkage analysis. The effects of the candidate gene polymorphisms were analyzed in two ways. When an animal model was fitted, both genes showed significant effects on fatness traits, the H-FABP polymorphism showed significant effects on IMF and MA, and the LEPR polymorphism on BF and IMF. But when the candidate gene effect was included in a QTL regression analysis these associations were not observed, suggesting that they must not be the causal mutations responsible for the effects found. Differences in the results of both analyses showed the inadequacy of the animal model approach for the evaluation of positional candidate genes in populations with linkage disequilibrium, when the probabilities of the parental origin of the QTL alleles are not included in the model.

Keywords

candidate gene H-FABP LEPR QTL pigs 

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Copyright information

© INRA, EDP Sciences 2002

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Authors and Affiliations

  • Óvilo Cristina
    • 1
    Email author
  • Angels Oliver
    • 2
  • José Luis Noguera
    • 3
  • Alex Clop
    • 4
  • Carmen Barragán
    • 1
  • Luis Varona
    • 3
  • Carmen Rodríguez
    • 1
  • Miguel Toro
    • 1
  • Armand Sánchez
    • 4
  • Miguel Pérez-Enciso
    • 3
  • Luis Silió
    • 1
  1. 1.Departamento de Mejora Genética AnimalSGIT-INIAMadridSpain
  2. 2.Centre de Tecnologia de la Carn, IRTAMonells, GironaSpain
  3. 3.Area de Producció Animal, Centre UdL-IRTALleidaSpain
  4. 4.Departament de Ciència Animal i dels AlimentsUniversitat Autònoma de BarcelonaBellaterra, BarcelonaSpain

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