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Genetics Selection Evolution

, 34:105 | Cite as

Association of a missense mutation in the bovine leptin gene with carcass fat content and leptin mRNA levels

  • Fiona C. BuchananEmail author
  • Carolyn J. Fitzsimmons
  • Andrew G. Van Kessel
  • Tracey D. Thue
  • Dianne C. Winkelman-Sim
  • Sheila M. Schmutz
Open Access
Research

Abstract

Previously, we have shown that alleles of the BM1500 microsatellite, located 3.6 kb downstream of the leptin gene in cattle, were associated with carcass fat measures in a population of 154 unrelated beef bulls. Subsequently, a cytosine (C) to thymine (T) transition that encoded an amino acid change of an arginine to a cysteine was identified in exon 2 of the leptin gene. A PCR-RFLP was designed and allele frequencies in four beef breeds were correlated with levels of carcass fat. The T allele was associated with fatter carcasses and the C allele with leaner carcasses. The frequencies of the SNP alleles among breeds indicated that British breeds have a higher frequency of the T allele whereas the continental breeds have a higher occurrence of the C allele. A ribonuclease protection assay was developed to quantify leptin mRNA in a separate group of animals selected by genotype. Animals homozygous for thymine expressed higher levels of leptin mRNA. This may suggest that the T allele, which adds an extra cysteine to the protein, imparts a partial loss of biological function and hence could be the causative mutation.

Keywords

leptin cattle obese fat marbling 

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Copyright information

© INRA, EDP Sciences 2002

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Authors and Affiliations

  • Fiona C. Buchanan
    • 1
    Email author
  • Carolyn J. Fitzsimmons
    • 2
  • Andrew G. Van Kessel
    • 1
  • Tracey D. Thue
    • 1
  • Dianne C. Winkelman-Sim
    • 1
  • Sheila M. Schmutz
    • 1
  1. 1.Department of Animal and Poultry ScienceUniversity of SaskatchewanSaskatoonCanada
  2. 2.Department of Animal ScienceIowa State UniversityAmesUSA

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