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Genetic diversity in Spanish donkey breeds using microsatellite DNA markers

  • José Aranguren-Méndez
  • Jordi JordanaEmail author
  • Mariano Gomez
Open Access
Research

Abstract

Genetic diversity at 13 equine microsatellite loci was compared in five endangered Spanish donkey breeds: Andaluza, Catalana, Mallorquina, Encartaciones and Zamorano-Leonesa. All of the equine microsatellites used in this study were amplified and were polymorphic in the domestic donkey breeds with the exception of HMS1, which was monomorphic, and ASB2, which failed to amplify. Allele number, frequency distributions and mean heterozygosities were very similar among the Spanish donkey breeds. The unbiased expected heterozygosity (HE) over all the populations varied between 0.637 and 0.684 in this study. The low GST value showed that only 3.6% of the diversity was between breeds (P < 0.01). Significant deviations from Hardy-Weinberg equilibrium were shown for a number of locus-population combinations, except HMS5 that showed agreement in all analysed populations. The cumulative exclusion probability (PE) was 0.999 in each breed, suggesting that the loci would be suitable for donkey parentage testing. The constructed dendrogram from the DA distance matrix showed little differentiation between Spanish breeds, but great differentiation between them and the Moroccan ass and also with the horse, used as an outgroup. These results confirm the potential use of equine microsatellite loci as a tool for genetic studies in domestic donkey populations, which could also be useful for conservation plans.

Keywords

donkey endangered breed microsatellite diversity genetic variability 

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Copyright information

© INRA, EDP Sciences 2001

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Authors and Affiliations

  • José Aranguren-Méndez
    • 1
    • 2
  • Jordi Jordana
    • 1
    Email author
  • Mariano Gomez
    • 3
  1. 1.Unitat de Genètica i Millora Animal, Departament de Ciència Animal i dels Aliments, Facultat de VeterinàriaUniversitat Autònoma de BarcelonaBellaterra, BarcelonaSpain
  2. 2.Facultad de Ciencias Veterinarias, Departamento de Producción AnimalUniversidad del ZuliaMaracaiboVenezuela
  3. 3.Servicio de GanaderíaDiputación Foral de BizkaiaBilbaoSpain

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