Advertisement

Reproductive Sciences

, Volume 18, Issue 12, pp 1222–1229 | Cite as

Expression of Keratinocyte Growth Factor in Goat Ovaries and Its Effects on Preantral Follicles Within Cultured Ovarian Cortex

  • L. R. FaustinoEmail author
  • R. Rossetto
  • I. M. T. Lima
  • C. M. G. Silva
  • M. V. A. Saraiva
  • L. F. Lima
  • A. W. B. Silva
  • M. A. M. Donato
  • C. C. Campello
  • C. A. Peixoto
  • J. R. Figueiredo
  • A. P. R. Rodrigues
Original Articles

Abstract

The aims of this study were to evaluate the expression of keratinocyte growth factor (KGF) in goat ovaries and to study its effects on preantral follicle survival and development. The ovaries were used for immunohistochemistry or for in vitro culture for 1 or 7 days with KGF (0, 1, 10, 50, 100, 150, or 200 ng/mL). Noncultured (fresh control) and cultured ovarian slices were processed for histological analysis and transmission electron microscopy (TEM). The results showed that after 7 days of in vitro culture, all treatments had a significant reduction in the percentage of normal follicles compared with the fresh control. After 7 days of culture, the highest KGF concentrations (150 and 200 ng/mL) induced a significant reduction in the percentage of normal follicles compared with the tissues cultured in the absence (α-MEM+ alone) or presence of 1, 10, and 50 ng/mL KGF. Transmission electron microscopy confirmed follicular integrity after 7 days of culture in 1 ng/mL KGF. In addition, compared with the fresh control, the percentage of growing follicles was significantly increased in all treatments after 1 or 7 days of culture. Immunohistochemical analyses showed the expression of KGF in oocytes and granulosa cells in all follicle developmental stages as well as in thecal and stromal cells. In conclusion, this study demonstrated that, at the lowest concentration (1 ng/mL), KGF maintained the ultrastructure of goat preantral follicles cultured in vitro for up to 7 days. Furthermore, the KGF protein was widely distributed in goat ovaries, especially in ovarian follicles.

Keywords

KGF follicle survival folliculogenesis ovary 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Hirshfield AN. Development of follicles in the mammalian ovary. Int Rev Cytol. 1991;124:43–101.Google Scholar
  2. 2.
    Adashi EY. Endocrinology of the ovary. Hum Reprod. 1994;9(5):36–51.Google Scholar
  3. 3.
    McGee EA, Hsueh AJ. Initial and cyclic recruitment of ovarian follicles. Endocr Rev. 2000;21(2):200–214.Google Scholar
  4. 4.
    Nayudu PL, Fehrenbach A, Kiesel P, Vitt UA, Pancharatna K, Osborn S. Progress toward understanding follicle development in vitro appearances are not deceiving. Arch Med Res. 2001;32(6):587–594.PubMedGoogle Scholar
  5. 5.
    Picton HM, Harris SE, Muruvi W, Chambers EL. The in vitro growth and maturation of follicles. Reproduction. 2008;136(6):703–715.Google Scholar
  6. 6.
    Picton H, Briggs D, Gosden R. The molecular basis of oocyte growth and development. Mol Cell Endocrinol. 1998;145(l-2):27–37.Google Scholar
  7. 7.
    Rubin JS, Bottaro DP, Chedid M, et al. Keratinocyte growth factor. Cell Biol Int. 1995;19(5):399–411.Google Scholar
  8. 8.
    Imagawa W, Cunha GR, Young P, Nandi S. Keratinocyte growth factor and acidic fibroblast growth factor are mitogens for primary cultures of mammary epithelium. Biochem Biophys Res Commun. 1994;204(3):1165–1169.Google Scholar
  9. 9.
    Casey ML, MacDonald PC. Keratinocyte growth factor expression in the mesenchymal cells of human amnion. J Clin Endocrinol Metab. 1997;82(18):3319–3323.Google Scholar
  10. 10.
    Werner S, Smola H, Liao X, et al. The function of KGF in morphogenesis of epithelium and reepithelialization of wounds. Science. 1994;266(5186):819–822.Google Scholar
  11. 11.
    Yi ES, Salgado M, Williams S, et al. Keratinocyte growth factor decreases pulmonary edema, transforming growth factor-b and platelet-derived growth factor-BB expression, and alveolar type II cell loss in bleomycin-induced lung injury. Inflammation. 1998;22(3):315–325.Google Scholar
  12. 12.
    Finch PW, Cunha GR, Rubin JS, Wong J, Ron D. Pattern of keratinocyte growth factor and keratinocyte growth factor receptor expression during mouse fetal development suggests a role in mediating morphogenetic mesenchymal-epithelial interactions. Dev Dyn. 1995;203(2):223–240.Google Scholar
  13. 13.
    Parrott JA, Vigne JL, Chu BZ, Skinner MK. Mesenchymal-epithelial interactions in the ovarian follicle involve keratinocyte and hepatocyte growth factor production by thecal cells and their action on granulosa cells. Endocrinology. 1994;135(2):569–575.Google Scholar
  14. 14.
    Kezele P, Nilsson EE, Skinner MK. Keratinocyte growth factor acts as a mesenchymal factor that promotes ovarian primordial to primary follicle transition. Biol Reprod. 2005;73(5):967–973.Google Scholar
  15. 15.
    McGee EA, Chun S-Y, Lai S, He Y-E, Hsueh AJW. Keratinocyte growth factor promotes the survival, growth, and differentiation of preantral ovarian follicles. Fertil Steril. 1999;71(4):732–738.Google Scholar
  16. 16.
    Chaves RN, Martins FS, Saraiva MV, et al. Chilling ovarian fragments during transportation improves viability and growth of goat preantral follicles cultured in vitro. Reprod Fertil Dev. 2008;20(5):640–647.Google Scholar
  17. 17.
    Bruno JB, Celestino JJH, Lima-Verde IB, et al. Expression of vascular endothelial growth factor (VEGF) receptor in goat ovaries and improvement of in vitro caprine preantral follicle survival and growth with VEGF. Reprod Fertil Dev. 2009;21(5):679–687.Google Scholar
  18. 18.
    Donato MAM, Saraiva KLA, Silva AKS, Wanderley MI, Peixoto CA. Follicle development and luteal cell morphology altered by phosphodiesterase-5 inhibitor. Micron. 2009;40(8):845–850.Google Scholar
  19. 19.
    Silva JRV, van den Hurk R, Matos MHT, et al. Influences of FSH and EGF on primordial follicles during in vitro culture of caprine ovarian cortical tissue. Theriogenology. 2004;61(9):1691–1704.Google Scholar
  20. 20.
    Steel RGD, Torrie JH, Dickey DA. Principles and Procedures of Statistics: A Biometrical Approach. 3rd ed. New York, NY: McGraw-Hill; 1997.Google Scholar
  21. 21.
    Salehnia M, Moghadam EA, Velojerdi MR. Ultrastructure of follicles after vitrification of mouse ovarian tissue. Fertil Steril. 2002;78(3):644–645.Google Scholar
  22. 22.
    Wang LH, Mullen SF, Li Y, Zhong JQ, Crister JK, Chen ZJ. Morphological and apoptotic comparison of primordial and primary follicles in cryopreserved human ovarian tissue. Reprod Domest Anim. 2009;44(6):879–883.Google Scholar
  23. 23.
    Magoffin DA. Ovarian theca cell. Int J Biochem Cell Biol. 2005;37(7):1344–1349.Google Scholar
  24. 24.
    Silva JRV, Tharasanit T, Taverne MAM, et al. The activin-follistatin system and in vitro early follicle development in goats. J Endocrinol. 2006;189(1):113–125.Google Scholar
  25. 25.
    Hartshorne GM. In vitro culture of ovarian follicles. Rev Reprod. 1997;2(2):94–104.Google Scholar
  26. 26.
    Chaves RN, Alves AMCV, Duarte ABG, et al. Nerve growth factor promotes the survival of goat preantral follicles cultured in vitro. Cells Tissues Organs. 2010;192(4):272–282.Google Scholar
  27. 27.
    Abir R, Fisch B, Zhang XY, et al. Keratinocyte growth factor and its receptor in human ovaries from fetuses, girls and women. Mol Hum Reprod. 2009;15(2):69–75.Google Scholar
  28. 28.
    Berisha B, Sinowatz F, Schams D. Expression and localization of fibroblast growth factor (FGF) family members during the final growth of bovine ovarian follicles. Mol Reprod Dev. 2004;67(2):162–171.Google Scholar

Copyright information

© Society for Reproductive Investigation 2011

Authors and Affiliations

  • L. R. Faustino
    • 1
    • 4
    Email author
  • R. Rossetto
    • 1
  • I. M. T. Lima
    • 1
  • C. M. G. Silva
    • 1
  • M. V. A. Saraiva
    • 1
  • L. F. Lima
    • 1
  • A. W. B. Silva
    • 2
  • M. A. M. Donato
    • 3
  • C. C. Campello
    • 1
  • C. A. Peixoto
    • 3
  • J. R. Figueiredo
    • 1
  • A. P. R. Rodrigues
    • 1
  1. 1.Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of VeterinaryState University of CearáFortalezaBrazil
  2. 2.Biotechnology Nucleus of Sobral (NUBIS)Federal University of CearáSobralBrazil
  3. 3.Laboratory of Ultrastructure, CPqAM/FIOCRUZFederal University of PernambucoRecife-PEBrazil
  4. 4.Programa de Pós-Graduação em Ciências Veterinárias (PPGCV), Laboratório de Manipulação de Oócitos e Folículos Pré-Antrais (LAMOFOPA)Universidade Estadual do Ceará (UECE)FortalezaBrazil

Personalised recommendations