Reproductive Sciences

, Volume 18, Issue 4, pp 359–373 | Cite as

Na+,K+-ATPase Activity and Subunit Protein Expression: Ontogeny and Effects of Exogenous and Endogenous Steroids on the Cerebral Cortex and Renal Cortex of Sheep

  • Chang-Ryul Kim
  • Grazyna B. Sadowska
  • Stephanie A. Newton
  • Maricruz Merino
  • Katherine H. Petersson
  • James F. Padbury
  • Barbara S. StonestreetEmail author
Original Articles


We examined the effects of development, exogenous, and endogenous glucocorticoids on Na+,K+-ATPase activity and subunit protein expression in ovine cerebral cortices and renal cortices. Ewes at 60%, 80%, and 90% gestation, newborns, and adults received 4 dexamethasone or placebo injections. Cerebral cortex Na+,K+-ATPase activity was higher (P <.05) in placebo-treated newborns than fetuses of placebo-treated ewes and adults, α1-expression was higher at 90% gestation than the other ages; α2-expression was higher in newborns than fetuses; α3-expression was higher in newborns than 60% gestation; β1-expression was higher in newborns than the other ages, and β2-expression higher at 60% than 80% and 90% gestation, and in adults. Renal cortex Na+,K+-ATPase activity was higher in placebo-treated adults and newborns than fetuses. Cerebral cortex Na+,K+-ATPase activity was higher in dexamethasone- than placebo-treated adults, and α1-expression higher in fetuses of dexamethasone- than placebo-treated ewes at 60% and 80% gestation. Renal cortex Na+,K+-ATPase activity and α1-expression were higher in fetuses of dexamethasone-than placebo-treated ewes at each gestational age, and β1-expression was higher in fetuses of dexamethasone-than placebo-treated ewes at 90% gestation and in dexamethasone-than placebo-treated adults. Cerebral cortex Na+,K+-ATPase activity, α1-expression, β1-expression, and renal cortex α1-expression correlated directly with increases in fetal cortisol. In conclusion, Na+,K+-ATPase activity and subunit expression exhibit specific developmental patterns in brain and kidney; exogenous glucocorticoids regulate activity and subunit expression in brain and kidney at some ages; endogenous increases in fetal cortisol regulate cerebral Na+,K+-ATPase, but exogenous glucocorticoids have a greater effect on renal than cerebral Na+,K+-ATPase.


brain kidney glucocorticoids Na+,K+ 


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Copyright information

© Society for Reproductive Investigation 2011

Authors and Affiliations

  • Chang-Ryul Kim
    • 1
    • 2
  • Grazyna B. Sadowska
    • 1
  • Stephanie A. Newton
    • 1
  • Maricruz Merino
    • 1
  • Katherine H. Petersson
    • 1
  • James F. Padbury
    • 1
  • Barbara S. Stonestreet
    • 1
    Email author
  1. 1.Department of Pediatrics, Women and Infants Hospital of Rhode IslandThe Warren Alpert Medical School of Brown UniversityProvidenceUSA
  2. 2.Department of PediatricsHanyang University College of MedicineSeoulKorea

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