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Diffraction studies on natural and model lipid bilayers

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Abstract

In this study we have used neutron diffraction to examine the swelling behaviour and bilayer parameters of membranes reconstituted from polar lipids extracted from B. subtilis and model systems composed of synthetic phospholipids. Evidence for phase separation in the model system (lacking in Lysyl-PG, L-PG) is discussed in relation to its possible contribution to membrane domain formation through lipid-lipid interactions. Comparing these results with those obtained from the bilayers composed of lipids extracted from bacterial cells gives us some indication of the role of L-PG in the B. subtilis plasma membrane.

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Authors and Affiliations

  1. Institut Laue Langevin, 6 rue Jules Horowitz, BP. 156, 38042, Grenoble, France

    F. Sebastiani, S. Khanniche, J.-B. Artero, M. Haertlein & G. Fragneto

  2. Dipartimento di Fisica, Università di Roma “Tor Vergata”, via della Ricerca Scientifica 1, 00133, Roma, Italy

    F. Sebastiani

  3. Institute of Pharmaceutical Science, King’s College London, Franklin-Wilkins Building, 150 Stamford Street, London, SE1 9NH, UK

    R. Harvey

  4. Macromolecular Structure Research Group, Keele University, Keele, Staffordshire, UK

    J.-B. Artero

Authors
  1. F. Sebastiani
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  2. R. Harvey
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  3. S. Khanniche
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  4. J.-B. Artero
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  5. M. Haertlein
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  6. G. Fragneto
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Correspondence to R. Harvey.

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Sebastiani, F., Harvey, R., Khanniche, S. et al. Diffraction studies on natural and model lipid bilayers. Eur. Phys. J. Spec. Top. 213, 355–365 (2012). https://doi.org/10.1140/epjst/e2012-01682-3

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  • DOI: https://doi.org/10.1140/epjst/e2012-01682-3

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