The European Physical Journal Special Topics

, Volume 167, Issue 1, pp 185–189 | Cite as

Characterizing the structure of protein layers adsorbed onto functionalized surfaces by means of in-situ X-ray reflectivity

  • F. Evers
  • K. Shokuie
  • M. Paulus
  • S. Tiemeyer
  • Ch. Sternemann
  • C. Czeslik
  • M. Tolan
Regular Article

Abstract

In a biological cell, proteins face a highly complex environment comprising crowding and confinement effects as well as interactions with interfaces, cosolvents, and other biomolecules. In this study, the X-ray reflectivity technique has been used for the in-situ characterization of adsorbed protein layers at solid-liquid interfaces. The adsorption of bovine serum albumin at the hydrophobic polystyrene-water interface has been investigated in the presence and in the absence of salts. The data indicate that enhanced adsorption occurs at high salt concentrations.

Keywords

European Physical Journal Special Topic Adsorbed Layer High Salt Concentration Protein Layer Bovine Serum Albumin Molecule 

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References

  1. D.K. Eggers, J.S. Valentine, Protein Sci. 10, 250 (2001) Google Scholar
  2. A.P. Minton, J. Cell Sci. 119, 2863 (2006) Google Scholar
  3. C. Czeslik, Z. Phys. Chem. 218, 771 (2004) Google Scholar
  4. D.G. Castner, B.D. Ratner, Surf. Sci. 500, 28 (2002) Google Scholar
  5. B. Kasemo, Surf. Sci. 500, 656 (2002) Google Scholar
  6. D.S. Wilson, S. Nock, Angew. Chem. Int. Ed. 42, 494 (2003) Google Scholar
  7. M. Tolan, X-ray scattering from Soft Matter Thin Films (Springer, Berlin, 1999) Google Scholar
  8. F. Evers, K. Shokuie, M. Paulus, C. Sternemann, C. Czeslik, M. Tolan, Langmuir 24, 10216 (2008) Google Scholar
  9. M. Paulus, D. Lietz, C. Sternemann, K. Shokuie, F. Evers, M. Tolan, C. Czeslik, R. Winter, J. Syn. Rad. 15, 600 (2008) Google Scholar
  10. C.E. Miller, J. Majewski, T. Gog, T.L. Kuhl, Phys. Rev. Lett. 94, 238104 (2005) Google Scholar
  11. M. Mezger, H. Reichert, S. Schröder, J. Okasinski, H. Schröder, H. Dosch, D. Palms, J. Ralston, J. Honkimäki, PNAS 103, 18401 (2006) Google Scholar
  12. H. Reichert, O. Klein, H. Dosch, M. Denk, V. Honkimäki, T. Lippmann, G. Reiter, Nature 408, 839 (2000) Google Scholar
  13. J. Koo, T. Gutberlet, C. Czeslik, J. Phys. Chem. B 112, 6292 (2008) Google Scholar
  14. L.D. Unsworth, H. Sheardown, J.L. Brash, Langmuir 24, 1924 (2008) Google Scholar
  15. S.N. Timasheff, Annu. Rev. Biophys. Biomol. Struct. 22, 67 (1993) Google Scholar
  16. W. Kunz, P. Lo Nostro, B.W. Ninham, Curr. Opin. Coll. Interf. Sci. 9, 1 (2004) Google Scholar
  17. B.J. Bennion, V. Daggett, PNAS 100, 5142 (2003) Google Scholar
  18. N. Javid, K. Vogtt, C. Krywka, M. Tolan, R. Winter, Chem. Phys. Chem. 8, 679 (2007) Google Scholar
  19. P. Koelsch, P. Viswanath, H. Motschmann, V.L. Shapovalov, G. Brezesinski, H. Möhwald, D. Horinek, R.R. Netz, K. Giewekemeyer, T. Salditt, H. Schollmeyer, R.V. Klitzing, J. Daillant, P. Guenoun, Coll. Surf. A 303, 110 (2007) Google Scholar
  20. D. Voet, J.G. Voet, Biochemistry, 3rd edn. (Wiley & Sons, New York, 2004) Google Scholar
  21. D.C. Carter, J.X. Ho, Adv. Prot. Chem. 45, 153 (1994) Google Scholar
  22. T. Peters, Adv. Prot. Chem. 37, 161 (1985) Google Scholar
  23. D. Bendedouch, S.-H. Chen, J. Phys. Chem. 87, 1473 (1983) Google Scholar
  24. O. Hollmann, T. Gutberlet, C. Czeslik, Langmuir 23, 1347 (2007) Google Scholar
  25. T.J. Su, J.R. Lu, R.K. Thomas, Z.F. Cui, J. Phys. Chem. B 103, 3727 (1999) Google Scholar
  26. T.J. Su, J.R. Lu, R.K. Thomas, Z.F. Cui, J. Penfold, J. Phys. Chem. B 102, 8100 (1998) Google Scholar
  27. C. Reich, M.B. Hochrein, B. Krause, B. Nickel, Rev. Sci. Instrum. 76, 095103 (2005) Google Scholar
  28. C. Krywka, M. Paulus, C. Sternemann, M. Volmer, A. Remhof, G. Nowak, A. Nefedov, B. Pöter, M. Spiegel, M. Tolan, J. Syn. Rad. 13, 8 (2006) Google Scholar
  29. L.G. Parratt, Phys. Rev. 95, 359 (1954) Google Scholar
  30. M.K. Sanyal, J.K. Basu, A. Datta, S. Banerjee, Europhys. Lett. 36, 265 (1996) Google Scholar
  31. O.H. Seeck, I.D. Kaendler, M. Tolan, K. Shin, M.H. Rafailovich, J. Sokolov, R. Kolb, Appl. Phys. Lett. 76, 2713 (2000) Google Scholar
  32. M. Malmsten, J. Coll. Interf. Sci. 207, 186 (1998) Google Scholar
  33. D. Chandler, Nature 437, 640 (2005) Google Scholar
  34. T.J. Su, J.R. Lu, R.K. Thomas, Z.F. Cui, J. Penfold, J. Coll. Interf. Sci. 203, 419 (1998) Google Scholar

Copyright information

© EDP Sciences and Springer 2009

Authors and Affiliations

  • F. Evers
    • 1
  • K. Shokuie
    • 1
  • M. Paulus
    • 1
  • S. Tiemeyer
    • 1
  • Ch. Sternemann
    • 1
  • C. Czeslik
    • 2
  • M. Tolan
    • 1
  1. 1.Fakultät Physik/DELTA, TU Dortmund, Maria-Goeppert-Mayer-Str. 2DortmundGermany
  2. 2.Fakultät Chemie, TU Dortmund, Otto-Hahn-Str. 6DortmundGermany

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