Skip to main content
Log in

The macromolecular crystallography beamlines at BESSY II of the Helmholtz-Zentrum Berlin: Current status and perspectives

  • Regular Article
  • Published:
The European Physical Journal Plus Aims and scope Submit manuscript

Abstract

For a little over a decade now, the Macromolecular Crystallography (MX) group at the Helmholtz-Zentrum Berlin (HZB) has been operating three state-of-the-art synchrotron beamlines for MX at the BESSY II storage ring in Berlin. The three HZB-MX beamlines, BL14.1, BL14.2 and BL14.3, serve a stable and growing user community of currently more than 100 independent research groups from Berlin, Germany and Europe. Every year, the beamlines provide close to 200 days of MX-beamtime. Over time, the HZB-MX beamlines and endstations, in particular BL14.1, have been continually developed and upgraded and, since 2010, they operate as the most productive MX beamlines in Germany. The environment of the beamlines includes various ancillary equipment as well as additional facilities, such as office space adjacent to the beamlines, a sample preparation laboratory, a safety level 1 biology laboratory (HZB-MX BioLab) and all necessary computing resources. In this paper, the current status of the beamlines as well as the ongoing developments are described.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. J.C. Kendrew, G. Bodo, H.M. Dintzis, R.G. Parrish, H. Wyckoff, D.C. Phillips, Nature 181, 662 (1958).

    Article  ADS  Google Scholar 

  2. M.F. Perutz, M.G. Rossmann, A.F. Cullis, H. Muirhead, G. Will, A.C.T. North, Nature 185, 416 (1960).

    Article  ADS  Google Scholar 

  3. H.M. Berman, J. Westbrook, Z. Feng, G. Gilliland, T.N. Bha, H. Weissig, I.N. Shindyalov, P.E. Bourne, Nucleic Acids Res. 28, 235 (2000).

    Article  Google Scholar 

  4. U. Mueller, N. Darowski, M.R. Fuchs, R. Förster, M. Hellmig, K.S. Paithankar, S. Pühringer, M. Steffien, G. Zocher, M.S. Weiss, J. Synch. Radiat. 19, 442 (2012).

    Article  Google Scholar 

  5. U. Heinemann, K. Bussow, U. Mueller, P. Umbach, Acc. Chem. Res. 36, 157 (2003).

    Article  Google Scholar 

  6. M.R. Fuchs, K. Holldack, Reichardt, U. Mueller, Aip Conf. Proc. 879, 1006 (2007).

    Article  ADS  Google Scholar 

  7. P. Fischer, B. Reime, N. Stuebe, T. Pakendorf, A. Meents, Acta Cryst. A 68, s268 (2012).

    Article  Google Scholar 

  8. S. Russi, D.H. Juers, J. Sanchez-Weatherby, E. Pellegrini, E. Mossou, V.T. Forsyth, J. Huet, A. Gobbo, F. Felisaz, R. Moya, S.M. McSweeney, S. Cusack, F. Cipriani, M.W. Bowler, J. Struct. Biol. 175, 236 (2011).

    Article  Google Scholar 

  9. D.L. Schönfeld, R.B. Ravelli, U. Mueller, A. Skerra, J. Mol. Biol. 384, 393 (2008).

    Article  Google Scholar 

  10. M.W. Bowler, U. Mueller, M.S. Weiss, J. Sanchez-Weatherby, T.L.-M. Sorensen, M. Thunnissen, T. Ursby, A. Gobbo, S. Russi, M.G. Bowler, S. Brockhauser, O. Svensson, F. Cipriani, Cryst. Growth Des. 15, 1043 (2015).

    Article  Google Scholar 

  11. L. Malinauskaite, M. Quick, L. Reinhard, J.A. Lyons, H. Yano, J.A. Javitch, P. Nissen, Nat. Struct. Mol. Biol. 21, 1006 (2014).

    Article  Google Scholar 

  12. T. Moschetti, U. Mueller, J. Schulze, M. Brunori, B. Vallone, Biophys. J. 97, 1700 (2009).

    Article  ADS  Google Scholar 

  13. U. Mueller, G. Zocher, R. Förster, M. Hellmig, M. Steffien, M.S. Weiss, in preparation (2015).

  14. Collaborative Computational Project, Number 4 Acta Cryst. D 50, 760 (1994).

    Article  Google Scholar 

  15. P.D. Adams et al., Acta Cryst. D 66, 213 (2010).

    Article  Google Scholar 

  16. G.M. Sheldrick, Acta Cryst. D 66, 479 (2010).

    Article  Google Scholar 

  17. M. Krug, M.S. Weiss, U. Heinemann, U. Mueller, J. Appl. Cryst. 45, 568 (2012).

    Article  Google Scholar 

  18. J. Gabadinho et al., J. Synchr. Radiat. 17, 700 (2010).

    Article  Google Scholar 

  19. H. Xu, N. Sträter, W. Schröder, C. Böttcher, K. Ludwig, W. Saenger, Acta Cryst. D 59, 815 (2003).

    Article  Google Scholar 

  20. M.N. Schulz, J. Fanghänel, M. Schäfer, V. Badock, H. Briem, U. Boemer, D. Nguyen, M. Husemann, M.C. Hilllig, Acta Cryst. D 67, 156 (2011).

    Article  Google Scholar 

  21. M. Gertz, F. Fischer, G.T.T. Nguyen, M. Lakshminarasimhan, M. Schutkowski, M. Weyand, C. Steegborn, Proc. Natl. Acad. Sci USA 110, E2772 (2013).

    Article  ADS  Google Scholar 

  22. D.C. Rees, M. Congreve, C.W. Murray, R. Carr, Nat. Rev. Drug Discov. 3, 660 (2004).

    Article  Google Scholar 

  23. Z. Dauter, M. Dauter, E. de La Fortelle, G. Bricogne, G.M. Sheldrick, J. Mol. Biol. 289, 83 (1999).

    Article  Google Scholar 

  24. C. Mueller-Dieckmann, S. Panjikar, A. Schmidt, S. Mueller, J. Kuper, A. Geerlof, M. Wilmanns, R.K. Singh, P.A. Tucker, M.S. Weiss, Acta Cryst. D 63, 366 (2007).

    Article  Google Scholar 

  25. K. Lakomek, A. Dickmanns, U. Mueller, K. Kollmann, F. Deuschl, A. Berndt, T. Lubke, R. Ficner, Acta Cryst. D 65, 220 (2009).

    Article  Google Scholar 

  26. C.M. Haas, Y. Roske, J. Protze, G. Krause, M.S. Weiss, U. Heinemann, in preparation (2015).

  27. S. Puehringer, M. Hellmig, S. Liu, M.S. Weiss, M.C. Wahl, U. Mueller, Acta Cryst. F 68, 981 (2012).

    Article  Google Scholar 

  28. L.M. Svensson, A.S. Jemth, M. Desroses, O. Loseva, T. Helleday, T.M. Högbom, P. Stenmark, FEBS Lett. 585, 2617 (2011).

    Article  Google Scholar 

  29. H. Gad et al., Nature 508, 215 (2014).

    Article  ADS  Google Scholar 

  30. S. Frielingsdorf, J. Fritsch, A. Schmidt, M. Hammer, J. Löwenstein, E. Siebert, V. Pelmenschikov, T. Jaenicke, J. Kalms, Y. Rippers, F. Lendzian, I. Zebger, Ch. Teutloff, M. Kaupp, R. Bittl, P. Hildebrandt, B. Friedrich, O. Lenz, P. Scheerer, Nat. Chem. Biol. 10, 378 (2014).

    Article  Google Scholar 

  31. J. Fritsch, O. Lenz, B. Friedrich, Nat. Rev. Microbiol. 11, 106 (2013).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Uwe Mueller.

Additional information

Contribution to the Focus Point on “Status of third-generation synchrotron crystallography beamlines: An overview” edited by Gaston Garcia.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Mueller, U., Förster, R., Hellmig, M. et al. The macromolecular crystallography beamlines at BESSY II of the Helmholtz-Zentrum Berlin: Current status and perspectives. Eur. Phys. J. Plus 130, 141 (2015). https://doi.org/10.1140/epjp/i2015-15141-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1140/epjp/i2015-15141-2

Keywords

Navigation