Advertisement

Advantages of statistical analysis of giant vesicle flickering for bending elasticity measurements

  • P. Méléard
  • T. Pott
  • H. Bouvrais
  • J. H. Ipsen
Regular Article

Abstract

We show how to greatly improve precision when determining bending elasticity of giant unilamellar vesicles. Taking advantage of the well-known quasi-spherical model of liposome flickering, we analyze the full probability distributions of the configurational fluctuations instead of limiting the analysis to the second moment measurements only as usually done in previously published works. This leads to objective criteria to reject vesicles that do not behave according to the model. As a result, the confidence in the bending elasticity determination of individual vesicles that fit the model is improved and, consequently, the reproducibility of this measurement for a given membrane system. This approach uncovers new possibilities for bending elasticity studies like detection of minute influences by solutes in the buffer or into the membrane. In the same way, we are now able to detect the inhomogeneous behavior of giant vesicle systems such as the hazardous production of peroxide in bilayers containing fluorescent dyes.

Keywords

DPPE Membrane Tension Giant Vesicle Giant Unilamellar Vesicle Individual Vesicle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    G. Beblik, R.M. Servuss, W. Helfrich, J. Phys. (Paris) 46, 1773 (1985).CrossRefGoogle Scholar
  2. 2.
    H. Engelhardt, H.P. Duwe, E. Sackmann, J. Phys. (Paris) Lett. 46, L395 (1985).CrossRefGoogle Scholar
  3. 3.
    E. Evans, D. Needham, J. Phys. Chem. 91, 4219 (1987).CrossRefGoogle Scholar
  4. 4.
    J.F. Faucon et al., J. Phys. (Paris) 50, 2389 (1989).CrossRefGoogle Scholar
  5. 5.
    E. Evans, W. Rawicz, Phys. Rev. Lett. 64, 2094 (1990).ADSCrossRefGoogle Scholar
  6. 6.
    L.A. Bagatolli, BBA - Biomembranes 1758, 1541 (2006).CrossRefGoogle Scholar
  7. 7.
    L.A. Bagatolli, E. Gratton, Biophys. J. 78, 290 (2000).CrossRefGoogle Scholar
  8. 8.
    L.A. Bagatolli, E. Gratton, Biophys. J. 77, 2090 (1999).CrossRefGoogle Scholar
  9. 9.
    J. Korlach et al., Proc. Natl. Acad. Sci. U.S.A. 96, 8461 (1999).ADSCrossRefGoogle Scholar
  10. 10.
    L.R. Montes et al., Biophys. J. 93, 3548 (2007).ADSCrossRefGoogle Scholar
  11. 11.
    J. Bernardino de la Serna et al., J. Biol. Chem. 279, 40715 (2004).CrossRefGoogle Scholar
  12. 12.
    C. Dietrich et al., Biophys. J. 80, 1417 (2001).ADSCrossRefGoogle Scholar
  13. 13.
    I. Plasencia, L. Norlén, L.A. Bagatolli, Biophys. J. 93, 3142 (2007).ADSCrossRefGoogle Scholar
  14. 14.
    P. Méléard et al., Biophys. J. 72, 2616 (1997).CrossRefGoogle Scholar
  15. 15.
    S.L. Veatch, S.L. Keller, BBA - Mol. Cell Res. 1746, 172 (2005).Google Scholar
  16. 16.
    T. Pott, H. Bouvrais, P. Méléard, Chem. Phys. Lipids 154, 115 (2008).CrossRefGoogle Scholar
  17. 17.
    P. Méléard, L.A. Bagatolli, T. Pott, in Methods in Enzymology, edited by N. Düzgüneş, Vol. 465 (Academic Press, Burlington, 2009) p. 161Google Scholar
  18. 18.
    H. Bouvrais et al., Biophys. Chem. 137, 7 (2008).CrossRefGoogle Scholar
  19. 19.
    P. Walde et al., ChemBioChem 11, 848 (2010).CrossRefGoogle Scholar
  20. 20.
    T. Shimanouchi, H. Umakoshi, R. Kuboi, Langmuir 25, 4835 (2009).CrossRefGoogle Scholar
  21. 21.
    T.J. Politano et al., Colloid Surf. B 79, 75 (2010).CrossRefGoogle Scholar
  22. 22.
    K.S. Horger et al., J. Am. Chem. Soc. 131, 1810 (2009).CrossRefGoogle Scholar
  23. 23.
    W. Helfrich, Z. Naturforsch. 28c, 693 (1973).Google Scholar
  24. 24.
    F. Brochard, J.-F. Lennon, J. Phys. (Paris) 36, 1035 (1975).CrossRefGoogle Scholar
  25. 25.
    M.B. Schneider, J.T. Jenkins, W.W. Webb, Biophys. J. 45, 891 (1984).CrossRefGoogle Scholar
  26. 26.
    R.M. Servuss, W. Harbich, W. Helfrich, BBA - Biomembranes 436, 900 (1976).CrossRefGoogle Scholar
  27. 27.
    I. Bivas et al., J. Phys. (Paris) 48, 855 (1987).ADSCrossRefGoogle Scholar
  28. 28.
    H.P. Duwe, J. Käs, E. Sackmann, J. Phys. (Paris) 51, 945 (1990).CrossRefGoogle Scholar
  29. 29.
    L. Fernandez-Puente et al., Europhys. Lett. 28, 181 (1994).ADSCrossRefGoogle Scholar
  30. 30.
    J. Henriksen, A.C. Rowat, J.H. Ipsen, Eur. Biophys. J. 33, 732 (2004).CrossRefGoogle Scholar
  31. 31.
    P. Méléard et al., Europhys. Lett. 19, 267 (1992).CrossRefGoogle Scholar
  32. 32.
    P. Méléard et al., Biochimie 80, 401 (1998).CrossRefGoogle Scholar
  33. 33.
    J. Pécréaux et al., Eur. Phys. J. E 13, 277 (2004).CrossRefGoogle Scholar
  34. 34.
    E. Sackmann, H.P. Duwe, H. Engelhardt, Faraday Discuss. Chem. Soc. 81, 7132 (1986).CrossRefGoogle Scholar
  35. 35.
    M.B. Schneider, J.T. Jenkins, W.W. Webb, J. Phys. (Paris) 45, 1457 (1984).CrossRefGoogle Scholar
  36. 36.
    E. Evans, Biophys. J. 43, 27 (1983).ADSCrossRefGoogle Scholar
  37. 37.
    J.R. Henriksen, J.H. Ipsen, Eur. Phys. J. E 14, 149 (2004).CrossRefGoogle Scholar
  38. 38.
    W. Rawicz et al., Biophys. J. 79, 328 (2000).ADSCrossRefGoogle Scholar
  39. 39.
    L. Bo, R.E. Waugh, Biophys. J. 55, 509 (1989).ADSCrossRefGoogle Scholar
  40. 40.
    J. Song, R.E. Waugh, Biophys. J. 64, 1967 (1993).ADSCrossRefGoogle Scholar
  41. 41.
    R.E. Waugh, R.M. Hochmuth, Biophys. J. 52, 391 (1987).ADSCrossRefGoogle Scholar
  42. 42.
    R. Dimova, B. Pouligny, C. Dietrich, Biophys. J. 79, 340 (2000).CrossRefGoogle Scholar
  43. 43.
    C.H. Lee, W.C. Lin, J. Wang, Phys. Rev. E 64, 20901 (2001).ADSCrossRefGoogle Scholar
  44. 44.
    C.H. Lee, W.C. Lin, J. Wang, 2000 Conference on Lasers and Electro-Optics (Nice, 2000), p. 592Google Scholar
  45. 45.
    C.H. Lee, W.C. Lin, J. Wang, Opt. Commun. 135, 233 (1997).ADSCrossRefGoogle Scholar
  46. 46.
    M. Kummrow, W. Helfrich, Phys. Rev. A 44, 8356 (1991).ADSCrossRefGoogle Scholar
  47. 47.
    Y. Lyatskaya et al., Phys. Rev. E 63, 11907 (2000).CrossRefGoogle Scholar
  48. 48.
    J.P. Reeves, R.M. Dowben, J. Cell Physiol. 73, 49 (1969).CrossRefGoogle Scholar
  49. 49.
    H. Bouvrais et al., BBA - Biomembranes 1798, 1333 (2010).CrossRefGoogle Scholar
  50. 50.
    P. Méléard et al., Europhys. Lett. 19, 267 (1992).CrossRefGoogle Scholar
  51. 51.
    J. Genova et al., J. Optoelectron. Adv. Mater. 31, 68 (2005).Google Scholar
  52. 52.
    J. Genova et al., Bulg. J. Phys. 31, 68 (2004).Google Scholar
  53. 53.
    S.T. Milner, S.A. Safran, Phys. Rev. A 36, 4371 (1987).ADSCrossRefGoogle Scholar
  54. 54.
    G. Arfken, Mathematical Methods for Physicists, 3rd edition (Academic Press, Inc. Boston, 1985).Google Scholar
  55. 55.
    M.D. Mitov, Advances in Supramolecular Chemistry, edited by G.W. Gokel, Vol. II (Jai Press, Inc., Greenwich, 1992) p. 93Google Scholar
  56. 56.
    P. Méléard et al., Europhys. Lett. 11, 355 (1990).CrossRefGoogle Scholar
  57. 57.
    T. Pott, P. Méléard, Europhys. Lett. 59, 87 (2002).ADSCrossRefGoogle Scholar
  58. 58.
    W.H. Press, Numerical Recipes in C. The Art of Scientific Computing, 2nd edition (Cambridge University Press, New York, 1992).Google Scholar
  59. 59.
    W. Rawicz et al., Biophys. J. 94, 4725 (2008).ADSCrossRefGoogle Scholar
  60. 60.
    R. Rodriguez-Garcia et al., Phys. Rev. Lett. 102, 128101 (2009).ADSCrossRefGoogle Scholar
  61. 61.
    L.R. Arriaga et al., Eur. Phys. J. E 31, 105 (2010).CrossRefGoogle Scholar
  62. 62.
    H. Bouvrais et al., Biophys. J. 96, 161a (2009).ADSCrossRefGoogle Scholar
  63. 63.
    T. Honger et al., Phys. Rev. Lett. 72, 3911 (1994).ADSCrossRefGoogle Scholar
  64. 64.
    D. Marsh, Chem. Phys. Lipids 144, 146 (2006).CrossRefGoogle Scholar
  65. 65.
    A.G. Petrov, I. Bivas, Prog. Surf. Sci. 16, 389 (1984).ADSCrossRefGoogle Scholar
  66. 66.
    A.G. Ayuyan, F.S. Cohen, Biophys. J. 91, 2172 (2006).ADSCrossRefGoogle Scholar
  67. 67.
    G.W. Feigenson, BBA - Biomembranes 1788, 47 (2009).CrossRefGoogle Scholar
  68. 68.
    J. Yuan et al., J. Am. Chem. Soc. 130, 2067 (2008).CrossRefGoogle Scholar
  69. 69.
    J. Zhao et al., BBA - Biomembranes 1768, 2777 (2007).CrossRefGoogle Scholar

Copyright information

© EDP Sciences, SIF, Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  • P. Méléard
    • 1
  • T. Pott
    • 1
  • H. Bouvrais
    • 1
    • 2
  • J. H. Ipsen
    • 2
  1. 1.UMR CNRS-ENSCR 6226 “Sciences Chimiques de Rennes”, ENSCRUniversité Européenne de BretagneRennes Cedex 7France
  2. 2.Department of Physics and Chemistry, MEMPHYS - Center for Biomembrane PhysicsUniversity of Southern DenmarkOdenseDenmark

Personalised recommendations