Advertisement

Internal structures of agar-gelatin co-hydrogels by light scattering, small-angle neutron scattering and rheology

Regular Article

Abstract

Internal structures of agar-gelatin co-hydrogels were investigated as a function of their volumetric mixing ratio, \(\ensuremath r=[\mathrm{AG}]:[\mathrm{Ge}]=0.5\) , 1.0 and 2.0 using dynamic light scattering (DLS), small-angle neutron scattering (SANS) and rheology. The degree of non-ergodicity ( \(\ensuremath X=0.2\pm0.02\) , which was extracted as a heterodyne contribution from the measured dynamic structure factor data remained less than that of homogeneous solutions where ergodicity is expected (X = 1 . The static structure factor, I(q) , results obtained from SANS were interpreted in the Guinier regime (low-q , which implied the existence of \( \approx\) 250 nm long rod-like structures (double-helix bundles), and the power law (intermediate-q regions yielded \(\ensuremath I(q)\sim q^{-\alpha}\) , with \( \alpha\) = 2.3 , 1.8 and 1.6 for r = 0.5 , 1.0 and 2.0. This is indicative of the presence of Gaussian chains at low r , while at r = 2 there was a propensity of rod-shaped structures. The gel strength and transition temperatures measured from frequency sweep and temperature ramp studies were suggestive of the presence of a stronger association between the two biopolymer networks at higher r . The results indicate that the internal structures of agar-gelatin co-hydrogels were highly dependent on the volumetric mixing ratio.

Keywords

Gelatin Fractal Dimension Dynamic Light Scattering Dynamic Light Scattering Dynamic Structure Factor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    H.B. Bohidar, P.L. Dubin, Y. Osada, Polymer Gels: Fundamentals and Applications (American Chemical Society, 2002)Google Scholar
  2. 2.
    H. Tanaka, S. Jabbari-Farouji, J. Meunier, D. Bonn, Phys. Rev. E 71, 021402 (2005)ADSCrossRefGoogle Scholar
  3. 3.
    A. Fluerasu, Moussaid, A. Madsen, Schofield, Phys. Rev. E 76, 01040 (2007)CrossRefGoogle Scholar
  4. 4.
    Zheng Li, L.E. Buerkle, M.R. Orseno, K.A. Stretletzky, S. Seifert, A. M. Jamieson, S.J. Rowan, Langmuir 26, 10093 (2010)CrossRefGoogle Scholar
  5. 5.
    M.H. Nordby, Anna-Lena Kjoniksen, B. Nystrom, J. Roots, Biomacromolecules 4, 337 (2003)CrossRefGoogle Scholar
  6. 6.
    F. Ferri, M. Greco, G. Arcovito, F. Andresai Bassi, M. De Spirito, E. Paganini, M. Rocco, Phys. Rev. E 63, 031401 (2001)ADSCrossRefGoogle Scholar
  7. 7.
    S.S. Singh, V.K. Aswal, H.B. Bohidar, Int. J. Biol. Macromolecules 4, 301 (2007)CrossRefGoogle Scholar
  8. 8.
    A.B. Kayitmazer, H.B. Bohidar, K.M. Mattison, A. Bose, J. Sarkar, A. Hashidzume, P.S. Russo, W. Jaeger, P.L. Dubin, Soft Matter 3, 1064 (2007)ADSCrossRefGoogle Scholar
  9. 9.
    B. Mohanty, A. Gupta, S. Bandyopadhyay, H.B. Bohidar, J. Polym. Sci. Part B, Polym. Phys. 45, 1511 (2007)ADSCrossRefGoogle Scholar
  10. 10.
    B. Mohanty, V.K. Aswal, J. Kohlbrecher, H.B. Bohidar, J. Polym. Sci. Part B, Polym. Phys. 44, 1653 (2006)ADSCrossRefGoogle Scholar
  11. 11.
    A.B. Kayitmaze, S.P. Strand, C. Tribet, A. Bose, W. Jaeger, P.L. Dubin, Biomacromolecules 8, 3568 (2007)CrossRefGoogle Scholar
  12. 12.
    I.C. Dea, M. Mckinnon, D.A. Rees, J. Mol. Biol. 68, 153 (1972)CrossRefGoogle Scholar
  13. 13.
    D.L. Seigfried, J.A. Manson, L.H. Sperling, J. Polym. Sci. Polym. Phys. Ed. 16, 583 (1978)ADSCrossRefGoogle Scholar
  14. 14.
    A.H. Clark, R.K. Richardso, S.B. Ross-Murphy, J.M. Stubbs, Macromolecules 16, 1367 (1983)ADSCrossRefGoogle Scholar
  15. 15.
    M.J. Ridont, S. Garza, G.J. Brownsey, V.J. Morris, Int. J. Biol. Macromolecules 18, 5 (1996)CrossRefGoogle Scholar
  16. 16.
    C. Rochas, M. Rinando, S. Landry, Carbohyd. Polym. 10, 115 (1989)CrossRefGoogle Scholar
  17. 17.
    M.H. Norziah, S.L. Foo, A.A. Karim, Food Hydrocolloids 20, 204 (2006)CrossRefGoogle Scholar
  18. 18.
    F. Horkay, Colloid Polym. Sci. 135, 10 (2008)Google Scholar
  19. 19.
    A.H. Clark, R.K. Richardson, S.B. Ross-Murphy, J.M. Stubbs, Macromolecules 16, 1367 (1983)ADSCrossRefGoogle Scholar
  20. 20.
    M. Watase, K. Nishinari, Rheol. Acta 22, 580 (1982)CrossRefGoogle Scholar
  21. 21.
    A. Veis, The Macromolecular Chemistry of Gelatin (Academic Press, 1964)Google Scholar
  22. 22.
    E. Pines, W. Prins, Macromolecules 16, 888 (1972)Google Scholar
  23. 23.
    A.K. Siddhanta, R. Meena, K. Prasad, B.K. Ramavat, P.K. Ghosh, K. Eswaran, US Patent Publication No. US 2005/0267296 A1 (2005)Google Scholar
  24. 24.
    S. Boral, A. Saxena, H.B. Bohidar, J. Phys. Chem. B 112, 3625 (2008)CrossRefGoogle Scholar
  25. 25.
    J. Kohlbrecher, W. Wagner, J. Appl. Crystallogr. 33, 804 (2000)CrossRefGoogle Scholar
  26. 26.
    B. Jacort, G. Zaccai, Biopolymers 20, 2413 (1981)CrossRefGoogle Scholar
  27. 27.
    S. Romer, C. Urban, V. Lobaskin, F. Scheffold, A. Stradner, J. Kohlbrecher, P. Schurtenberger, J. Appl. Crystallogr. 36, 1 (2003)CrossRefGoogle Scholar
  28. 28.
    G.D. Wignall, F.S. Bates, J. Appl. Crystallogr. 20, 28 (1987)CrossRefGoogle Scholar
  29. 29.
    G.L. Squires, Thermal Neutron Scattering (Cambridge, 1987)Google Scholar
  30. 30.
    L.A. Feigin, D.I. Svergun, Structure Analysis by Small Angle X-ray and Neutron Scattering (Plenum, 1987)Google Scholar
  31. 31.
    T. Coviello, E. Geissler, D. Meier, Macromolecules 30, 2008 (1997)ADSCrossRefGoogle Scholar
  32. 32.
    S.S. Singh, V.K. Aswal, H.B. Bohidar, Polymer 50, 5589 (2009)CrossRefGoogle Scholar
  33. 33.
    L. Cipelletti, S. Manley, R.C. Ball, D.A. Weitz, Phys. Rev. Lett. 84, 2275 (2000)ADSCrossRefGoogle Scholar
  34. 34.
    H. Tanaka, S. Jabbari-Farouji, J. Meunier, D. Bonn, Phys. Rev. E. 69, 031404 (2004)ADSCrossRefGoogle Scholar
  35. 35.
    H. Tanaka, S. Jabbari-Farouji, J. Meunier, D. Bonn, Phys. Rev. E 71, 021402 (2005)ADSCrossRefGoogle Scholar
  36. 36.
    S.Z. Ren, C.M. Sorensen, Phys. Rev. Lett. 70, 1727 (1993)ADSCrossRefGoogle Scholar
  37. 37.
    P. Mayer, H. Bissing, L. Berthier, L. Cipellett, P. Garrahan Sollich, V. Trappe, Phys. Rev. Lett. 93, 115701 (2004)ADSCrossRefGoogle Scholar
  38. 38.
    H.B. Bohidar, Characterization of polyelectrolytes by dynamic light scattering, in Handbook of Polyelectrolytes-II (American Scientific Publishers, 2002)Google Scholar
  39. 39.
    S. Manley, L. Cipelletti, D.A. Weitz, Phys. Rev. Lett. 93, 108302 (2004)ADSCrossRefGoogle Scholar
  40. 40.
    J.W. Phair, J.C. Schluz, L.P. Aldrise, J.D. Smith, J. Am. Ceram. Soc. 87, 129 (2004)CrossRefGoogle Scholar
  41. 41.
    S. Krueger, A.P. Andrews, R. Nossal, Biophys. Chem. 53, 85 (1994)CrossRefGoogle Scholar
  42. 42.
    P. Jaya Prakash Yadav, A.K. Prata, P.U. Sastry, B.K. Ghorai, P. Maiti, J. Phys. Chem. B 114, 11420 (2010)CrossRefGoogle Scholar

Copyright information

© EDP Sciences, SIF, Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  • S. Santinath Singh
    • 1
  • V. K. Aswal
    • 2
  • H. B. Bohidar
    • 1
  1. 1.Polymer and Biophysics Laboratory, School of Physical SciencesJawaharlal Nehru UniversityNew DelhiIndia
  2. 2.Solid State Physics DivisionBhaba Atomic Research CentreMumbaiIndia

Personalised recommendations