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Comparison of cell repair mechanisms by means of chromosomal aberration induced by proton and gamma irradiation – preliminary results

  • A. Kowalska
  • K. Czerski
  • M. Kaczmarski
  • M. Lewocki
  • B. Masojć
  • A. Łukowiak
Open Access
Regular Article
Part of the following topical collections:
  1. Topical Issue: Nano-scale Insights into Ion-beam Cancer Therapy. Guest editors: Andrey V. Solov’yov, Nigel Mason, Paulo Limão-Vieira, Malgorzata Smialek-Telega

Abstract

DNA damage of peripheral blood lymphocytes exposed to gamma and proton irradiation is studied by means of chromosome aberrations to validate the efficiency of the repair mechanisms of individual cells. A new method based on an observed deviation from the Poisson statistics of the chromosome aberration number is applied for estimation of a repair factor (RF) defined as a ratio between originally damaged cells to the amount of finally observed aberrations. The repair factors are evaluated by studying the variance of individual damage factors in a collective of healthy persons at a given dose as well as by using the chi-square analysis for the dose-effect curves. The blood samples from fifteen donors have been irradiated by Co60 gamma rays and from nine persons by 150 MeV protons with different doses up to 2 Gy. A standard extraction of lymphocyte has been used whereby dicentrics, acentrics and rings have been scored under a microscope. The RF values determined for the proton radiation are slightly larger than for gamma rays, indicating that up to 70% DNA double strand breaks can be repaired.

Graphical abstract

Keywords

Gamma Irradiation Repair Mechanism Chromosome Aberration Linear Energy Transfer Proton Irradiation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© The Author(s) 2015

Authors and Affiliations

  • A. Kowalska
    • 1
    • 2
    • 3
  • K. Czerski
    • 1
  • M. Kaczmarski
    • 1
  • M. Lewocki
    • 1
    • 4
  • B. Masojć
    • 4
  • A. Łukowiak
    • 1
  1. 1.Institute of Physics, University of SzczecinSzczecinPoland
  2. 2.Maritime University of SzczecinSzczecinPoland
  3. 3.Joint Institute of Nuclear ResearchDubnaRussia
  4. 4.Departament of Oncology, Pomeranian Medical UniversitySzczecinPoland

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