Abstract
DNA damage of peripheral blood lymphocytes exposed to gamma and proton irradiation is studied by means of chromosome aberrations to validate the efficiency of the repair mechanisms of individual cells. A new method based on an observed deviation from the Poisson statistics of the chromosome aberration number is applied for estimation of a repair factor (RF) defined as a ratio between originally damaged cells to the amount of finally observed aberrations. The repair factors are evaluated by studying the variance of individual damage factors in a collective of healthy persons at a given dose as well as by using the chi-square analysis for the dose-effect curves. The blood samples from fifteen donors have been irradiated by Co60 gamma rays and from nine persons by 150 MeV protons with different doses up to 2 Gy. A standard extraction of lymphocyte has been used whereby dicentrics, acentrics and rings have been scored under a microscope. The RF values determined for the proton radiation are slightly larger than for gamma rays, indicating that up to 70% DNA double strand breaks can be repaired.
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N.I. Nakajima et al., PLoS One 8, e70107 (2013)
H. Nikjoo, P. O’Neill, D.T. Goodhead, M. Terrissol, Int. J. Radiat. Biol. 71, 467 (1997)
E. Surdutovich, A.V. Yakubovich, A. Solovyov, Sci. Rep. 3, 1289 (2013)
G. Schiwietz, K. Czerski, M. Roth, F. Staufenbiel, P.L. Grande, Nucl. Instrum. Methods Phys. Res. B 226, 683 (2004)
M. Toulemonde, E. Surdutovich, A.V. Solovyov, Phys. Rev. E 80, 031913 (2009)
A.C. Upton, Environmental Health Perspectives 52, 31 (1983)
Cytogenetic Dosimetry: Applications in Preparedness for and Response to Radiation Emergencies (IAEA, Vienna, 2011), vol. 8, p. 45
K. Sakata, M. Someya, Y. Matsumoto, M. Hareymana, Radiat. Med. 25, 433 (2007)
L. Mah, C. Orlowski, K. Ververis, R.S. Vasireddy, A. El-Osta, T.C. Karagiannis, Genome Integrity 2:3 (2011)
J.T. Dunnen, G.B. Omen, in Protocols in Human Molecular Genetics: Methods in Molecular Biology, edited by C. Mathew (The Humana Press Inc., Clifton, 1992), vol. 9, p. 313
A.A. Edwards, D.C. Lloyd, R.J. Purrott, Radiat. Environ. Biophys. 16, 89 (1979)
C.M.S. Lee, F. Famoye, Biometrical J. 38, 299 (1996)
K. Janardan, D. Schaeffer, Biometrical J. 19, 599(2007)
A. Kowalska, K. Czerski, M. Kaczmarski, M. Lewocki, B. Masojć, to be published in Rad. Res.
E. Gudowska-Nowak, R. Lee, E. Nasonova, S. Ritter, M. Scholz, Adv. Space Res. 39, 249 (2005)
E. Surdutovich, A.V. Solov’yov, Phys. Rev. E 82, 051915 (2010)
R.H. Ritchie, W. Brandt, P.M. Echenique, Phys. Rev. B 14, 4808 (1976)
L.E. Feinendegen, M. Pollycove, C.A. Sondhaus, Nonlinearity Biology, Toxicol. Med. 2, 143 (2004)
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Contribution to the Topical Issue “Elementary Processes with Atoms and Molecules in Isolated and Aggregated States”, edited by Friedrich Aumayr, Bratislav Marinkovic, Stefan Matejcik, John Tanis and Kurt H. Becker.
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Kowalska, A., Czerski, K., Kaczmarski, M. et al. Comparison of cell repair mechanisms by means of chromosomal aberration induced by proton and gamma irradiation – preliminary results. Eur. Phys. J. D 69, 79 (2015). https://doi.org/10.1140/epjd/e2015-50034-0
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DOI: https://doi.org/10.1140/epjd/e2015-50034-0