Polarization-shaped laser pulses for improved fluorescence anisotropy contrast

  • Georg Achazi
  • Nils Hermes
  • Alexander Patas
  • Daniel Tolksdorf
  • Albrecht Lindinger
Regular Article

Abstract

We present a method to change the fluorescence contrast between two dyes with high fluorescence anisotropy in the same solution under different polarization directions by using polarization-shaped pulses. First a theoretical relationship between contrast, anisotropy and polarization is derived for this case. Then the wavelength dependent polarization anisotropy and the change of contrast by phase-shaping for a solution of Rhodamine B and Stilbene 3 in glycerol is shown. Finally, the application of the polarization-shaped pulses is demonstrated.

Keywords

Optical Phenomena and Photonics 

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Copyright information

© EDP Sciences, SIF, Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Georg Achazi
    • 1
  • Nils Hermes
    • 1
  • Alexander Patas
    • 1
  • Daniel Tolksdorf
    • 1
  • Albrecht Lindinger
    • 1
  1. 1.Institut für ExperimentalphysikFreie Universität BerlinBerlinGermany

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