Proton magnetic resonance spectroscopy of neurometabolites in the hippocampi of aggressive and tame male rats

  • R. G. Gulevich
  • A. E. Akulov
  • S. G. Shikhevich
  • R. V. Kozhemyakina
Article
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Abstract

The amounts of major neurometabolites were studied by proton magnetic resonance spectrometry in the dorsal hippocampus of adult male rats of populations after a long period of selection for an increase and absence of an aggressive-fearful response to humans and of unselected vivarium-kept rats. Tame and unselected males showed no significant differences in the percentages of any neurometabolites studied with regard to their total amount. The differences in the proportions of some neurometabolites were found in aggressive vs. tame and in aggressive vs. unselected animals. Tame animals showed higher proportions of GABA, N-acetylaspartate (NAA), and choline derivatives and a lower proportion of phosphorylethanolamine than aggressive ones. It is likely that the elevated content of GABA, one of the main inhibitory neurotransmitters in the brain, lowers the excitation intensity in tame rats as compared to aggressive ones. In comparison to the unselected animals, aggressive rats demonstrated higher proportions of glutamine, aspartate, phosphorylethanolamine, and lactate and lower proportions of NAA and creatine + phosphocreatine. Aspartate is one of the main excitement transmitters, and its elevated percentage in the dorsal hippocampi of aggressive rats may favor more intense excitation than in unselected rats. In addition, the elevated proportion of glutamine in aggressive rats vs. tame rats may be indicative of (1) a metabolic disturbance in the glutamate–glutamine cycle, which links the neural and glial cells, and (2) a decrease in the activity of glutaminase, the enzyme converting glutamine to glutamic acid (GABA precursor). The reduced NAA proportion, together with an elevated proportion of glutamine in aggressive rats points to an impaired energy metabolism in comparison to unselected animals. The differences in the neurometabolite patterns between the hippocampi of the male rats of the unselected and aggressive populations suggest the existence of different neurobiological mechanisms governing the manifestation of aggression.

Keywords

proton magnetic resonance spectroscopy aggression rats GABA hippocampus N-acetylaspartate selection 

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Copyright information

© Pleiades Publishing, Ltd. 2016

Authors and Affiliations

  • R. G. Gulevich
    • 1
  • A. E. Akulov
    • 1
  • S. G. Shikhevich
    • 1
  • R. V. Kozhemyakina
    • 1
  1. 1.Institute of Cytology and Genetics, Siberian BranchRussian Academy of SciencesNovosibirskRussia

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