Russian Journal of Genetics: Applied Research

, Volume 5, Issue 5, pp 500–506 | Cite as

Cytokine gene polymorphisms and early pregnancy loss

  • E. V. Mashkina
  • K. A. Kovalenko
  • N. V. Fomina
  • T. P. Shkurat


A disbalance of pro- and antiinflammatory cytokines can negatively affect the early stages of human embryogenesis. The association between polymorphism of cytokine genes (–31C-T IL1β,–174G-C IL6,–308G-A TNFα, and–592C-A and–819C-T IL10) and pregnancy loss was studied. The analysis was performed on DNA samples from two groups of women with pregnancy loss: those with a missed abortion (MA) (n = 62) and those with a spontaneous abortion (SA) (n = 62). The control group included 114 women with normal pregnancy. Cytokine genotyping was performed using PCR with sequence-specific primers with the CNP-express kit (Lytech, Russian Federation). The increase in the frequency of heterozygotes for the–31C-T polymorphism of the IL1β gene among women with MA (58.1%) compared to those with SA (36.7%) was found. In the SA group, the frequency of heterozygotes for–591C-T of the IL10 gene was higher (56.7%) than in the control group (32.5%). The frequency of the–819T allele of IL10 gene among women with SA was higher than in the control group (0.33 vs 0.23). At the same time, the frequency of the–308A allele of the TNFα gene was the lowest in the SA group compared to those in the MA and the control group. Our data show that the risk of spontaneous abortion increases if the–592A and–819T alleles of the IL10 and–308G allele of TNFα are present in the genotype.


cytokines polymorphism pregnancy spontaneous abortion missed abortion 


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Copyright information

© Pleiades Publishing, Ltd. 2015

Authors and Affiliations

  • E. V. Mashkina
    • 1
  • K. A. Kovalenko
    • 1
  • N. V. Fomina
    • 1
  • T. P. Shkurat
    • 1
  1. 1.Southern Federal UniversityRostov-on-DonRussia

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