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Use of molecular markers in wheat breeding for resistance to leaf rust at the Lukyanenko Research Institute of Agriculture

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Russian Journal of Genetics: Applied Research

Abstract

Wheat accessions were genotyped with molecular markers linked to wheat leaf rust resistance genes Lr9, Lr10, Lr19, Lr24, Lr26, Lr34, and Lr37. They included 1920 wheat plants and 46 commercial varieties bred at the Lukyanenko Institute. Basically, the analyzed varieties had the inefficient gene Lr10, poorly efficient Lr26 and Lr34, or their combinations. The highly efficient genes Lr9 and Lr24 were not detected. The Lr19 gene, effective in the Krasnodar region, was identified in varieties Pallada and Yara. The resistance gene Lr37 was found in the Morozko variety. Within a short time, the F2 and F3 plants with the introgression of genes Lr9, Lr19, Lr24, and Lr37 were obtained. Accessions with combinations Lr24 +Lr37, Lr24 + Lr19, Lr24 + Lr9, Lr19 + Lr37, Lr37 + Lr9, and Lr19 + Lr9 were identified. Seven plants with the combination of three genes Lr37 + Lr19 + Lr9 and one with Lr37 + Lr24 + Lr9 were selected.

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References

  • Ablova, I.B., Bespalova, L.A., Kolesnikov, F.A., et al., Principles, methods, and results of winter wheat breeding for resistance to diseases at the Lukyanenko Agricultural Research Institute, Sb. Nauch. Tr. Krasnodar. NIISKh (Collected Scientific Papers of Krasnodar NIISKh), 2014, pp. 48–67.

    Google Scholar 

  • Bespalova, L.A., Vasil’ev, A.V., Ablova, I.B., et al., The Use of Molecular Markers in Wheat Breeding at the Lukyanenko Agricultural Research Institute, Russ. J. Genet.: Appl. Res., 2012, vol. 2, no. 4, pp. 286–290.

    Article  Google Scholar 

  • Bonnett, D.G., Rebetzke, G.J., and Spielmeyer, W., Strategies for efficient implementation of molecular markers in wheat breeding, Mol. Breeding, 2005, vol. 15, pp. 75–78.

    Article  CAS  Google Scholar 

  • Friebe, B., Jiang, J., Raupp, W.J., et al., Characterization of wheat-alien translocations conferring resistance to diseases and pests: current status, Euphytica, 1996, vol. 91, pp. 59–87.

    Article  Google Scholar 

  • Gul’tyaeva, E.I. and Baranova, O.A, Trends in variability of Puccinia triticina population under the influence of cultivated wheat varieties and effectiveness of Lr-genes in the major grain-producing regions of the Russian Federation, in Tekhnologiya sozdaniya i ispol’zovaniya sortov i gibridov s gruppovoi i kompleksnoi ustoichivost’yu k vrednym organizmam v zashchite rastenii (Technology of Creation and Use of Cultivars and Hybrids with Group and Combined Resistance to Pests in Plant Protection), St. Petersburg: RASKhN, Otd. Zashchity Rast., GNU VNIIZR, 2010, pp. 26–48.

    Google Scholar 

  • Gul’tyaeva, E.I., Metody identifikatsii genov ustoichivosti pshenitsy k buroi rzhavchine s ispol’zovaniem DNK-markerov i kharakteristika effektivnosti Lr-genov (Methods for Identifying Genes for Resistance to Stem Rust of Wheat Using Dna Markers and Characteristics of Efficiency of Lr Genes), St. Petersburg: RASKhN, Otd. Zashchity Rast., GNU VNIIZR, 2012.

    Google Scholar 

  • Helguera, M., Khan, I.A., Kolmer, J., et al., PCR assays for the Lr37-Yr17-Sr38 cluster of rust resistance genes and their use to develop isogenic hard red spring wheat lines, Crop Sci., 2003, vol. 43, pp. 1839–1847.

    Article  CAS  Google Scholar 

  • Lagudah, E.S., Mcfadden, H., Singh, R.P., et al., Molecular genetic characterization of the Lr34/Yr18 slow rusting resistance gene region in wheat, Theor. Appl. Genet., 2006, vol. 114, pp. 21–30.

    Article  CAS  PubMed  Google Scholar 

  • Leonova, I.N., Molecular markers: implementation in crop plant breeding for identification, introgression and gene pyramiding, Russ. J. Genet.: Appl. Res., 2013, vol. 3, no. 6, pp. 464–474.

    Article  Google Scholar 

  • Mcintosh, R.A., Wellings, C.R., and Park, R.F., Wheat Rust: an Atlas of Resistance Gene, CSIRO, Australia, 1995, pp. 234–237.

    Book  Google Scholar 

  • Mcintosh, R.A., Postulation of leaf (brown) rust resistance genes in 70 wheat cultivars grown in United Kingdom, Euphytica, 2001, vol. 120, pp. 205–218.

    Article  Google Scholar 

  • McIntosh, R.A., Yamazaki, Y., Dubcovsky, J., et al., Catalogue of Gene Symbols for Wheat, 2010, Suppl. 2011, 2012. Available at http://www.shigen.nig.ac.jp/wheat/komugi/genes/

    Google Scholar 

  • Nocente, F., Fritz, A.K., Moran, J.L., et al., Identification and molecular tagging of genes Lr1, Lr9, Lr24, Lr47 and their introgression into common wheat cultivars by marker-assisted selection, Euphytica, 2007, vol. 155, pp. 329–336.

    Article  CAS  Google Scholar 

  • Plaschke, J., Ganal, M.W., and Roder, M.S., Detection of genetic diversity in closely related bread wheat using microsatellite markers, Theor. Appl. Genet., 1995, vol. 91, pp. 1001–1007.

    CAS  PubMed  Google Scholar 

  • Prins, R., Groenewald, J.Z., Marais, G.F., et al., AFLPSTS tagging of Lr19, a gene conferring resistance to leaf rus tin wheat, Theor. Appl. Genet., 2001, vol. 91, pp. 618–624.

    Article  Google Scholar 

  • Prubhu, K.W. and Tiwary, R., Marker assisted breeding in wheat: rust and biotic stresses-I, in ICAR-ACIAR Planning Workshop, Oct. 11–13, 2007, NASC, New Delhi, 2007. http://aciar.gov.au./Files/node/3871/Session% 20IV-Prabhu%20Tiwari.pdf

    Google Scholar 

  • Schachermayer, G., Siedler, H., and Gale, M.D., Identification and localization of molecular markers linked to the Lr9 leaf rust resistance gene of wheat, Theor. Appl. Genet., 1994, vol. 88, pp. 110–115.

    Google Scholar 

  • Schachermayer, G., Messemer, M., Feuillet, C., et al., Identification of molecular markers linked to the Agropyron elongatum-derived leaf rust resistance gene Lr24 in wheat, Theor. Appl. Genet., 1995, vol. 90, pp. 982–990.

    Google Scholar 

  • Schachermayer, G., Feuillet, C., and Keller, B., Molecular markers for detection of the wheat leaf rust resistance gene Lr10 in diverse genetic backgrounds, Mol. Breeding, 1997, vol. 3, pp. 65–74.

    Article  Google Scholar 

  • Singh, R.P., Huerta-Espino, J., and Wiliam, M., Genetics and breeding for durable resistance to leaf and stripe rusts of wheat, in Increasing Wheat Production in Central Asia through Science and International Cooperation: Proc. 1st Central Asian Wheat Conf., Almaty, Kazakhstan, June 10–13, 2003, Almaty, 2003, pp. 127–132.

    Google Scholar 

  • Singh, D., Franks, C.D., Huang, L., et al., Lr41, Lr39, and a leaf rust resistance gene from Aegilops cylindrical may be allelic and are located on wheat chromosome 2DS, Theor. Appl. Genet., 2004, vol. 108, pp. 586–591.

    Article  CAS  PubMed  Google Scholar 

  • Sivasamy, M. Vinod, TiwariS., et al., Introgression of useful linked genes for resistance to stem rust, leaf rust and powdery mildew and their molecular validation in wheat (Triticum aestivum L.), Indian J. Genet., 2009, vol. 69, pp. 17–27.

    CAS  Google Scholar 

  • Slikova, S., Gregova, E., and Bartos, P., Development of wheat genotypes possessing a combination of leaf rust resistance genes Lr19 and Lr24, Plant Soil Environ., 2004, vol. 50, no. 10, pp. 434–438.

    CAS  Google Scholar 

  • Sydenham, S.L., Pyramiding wheat rust resistance genes using marker-assisted selection. Master’s theses, University of Free State, Republic of South Africa, 2007. Available at http://etd.uovs.ac.za./ETD-db//theses/available/etd-02052009-140213/iunrestricted/Sydenham S.L.pdf

    Google Scholar 

  • Timonova, E.M., Leonova, I.N., Roder, M.S., and Salina, E.A., Marker-assisted development and characterization of a set of Triticum aestivum lines carrying different introgressions from the T. timopheevii genome, Mol. Breed., 2013, vol. 31, pp. 123–136.

    Article  Google Scholar 

  • Vida, G., Gal, M., Uhrin, A., et al., Molecular markers for the identification of resistance genes and markerassisted selection in breeding wheat for leaf rust resistance, Euphytica, 2009, vol. 170, pp. 67–76.

    Article  CAS  Google Scholar 

  • Weng, Y., et al., PCR-based markers for detection of different sources 1AL.1RS and 1BL.1RS wheat–rye translocation in wheat background, Plant Breed., 2007, vol. 126.

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Correspondence to E. R. Davoyan.

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Original Russian Text © E.R. Davoyan, L.A. Bespalova, R.O. Davoyan, Yu.S. Zubanova, D.S. Mikov, V.A. Filobok, J.N. Khudokormova, 2014, published in Vavilovskii Zhurnal Genetiki i Selektsii, 2014, Vol. 18, No. 4/1, pp. 732–738.

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Davoyan, E.R., Bespalova, L.A., Davoyan, R.O. et al. Use of molecular markers in wheat breeding for resistance to leaf rust at the Lukyanenko Research Institute of Agriculture. Russ J Genet Appl Res 5, 227–232 (2015). https://doi.org/10.1134/S2079059715030041

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  • DOI: https://doi.org/10.1134/S2079059715030041

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