Use of molecular markers in wheat breeding for resistance to leaf rust at the Lukyanenko Research Institute of Agriculture

  • E. R. Davoyan
  • L. A. Bespalova
  • R. O. Davoyan
  • Yu. S. Zubanova
  • D. S. Mikov
  • V. A. Filobok
  • J. N. Khudokormova
Article

Abstract

Wheat accessions were genotyped with molecular markers linked to wheat leaf rust resistance genes Lr9, Lr10, Lr19, Lr24, Lr26, Lr34, and Lr37. They included 1920 wheat plants and 46 commercial varieties bred at the Lukyanenko Institute. Basically, the analyzed varieties had the inefficient gene Lr10, poorly efficient Lr26 and Lr34, or their combinations. The highly efficient genes Lr9 and Lr24 were not detected. The Lr19 gene, effective in the Krasnodar region, was identified in varieties Pallada and Yara. The resistance gene Lr37 was found in the Morozko variety. Within a short time, the F2 and F3 plants with the introgression of genes Lr9, Lr19, Lr24, and Lr37 were obtained. Accessions with combinations Lr24 +Lr37, Lr24 + Lr19, Lr24 + Lr9, Lr19 + Lr37, Lr37 + Lr9, and Lr19 + Lr9 were identified. Seven plants with the combination of three genes Lr37 + Lr19 + Lr9 and one with Lr37 + Lr24 + Lr9 were selected.

Keywords

common wheat leaf rust resistance genes molecular markers marker-assisted selection 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ablova, I.B., Bespalova, L.A., Kolesnikov, F.A., et al., Principles, methods, and results of winter wheat breeding for resistance to diseases at the Lukyanenko Agricultural Research Institute, Sb. Nauch. Tr. Krasnodar. NIISKh (Collected Scientific Papers of Krasnodar NIISKh), 2014, pp. 48–67.Google Scholar
  2. Bespalova, L.A., Vasil’ev, A.V., Ablova, I.B., et al., The Use of Molecular Markers in Wheat Breeding at the Lukyanenko Agricultural Research Institute, Russ. J. Genet.: Appl. Res., 2012, vol. 2, no. 4, pp. 286–290.CrossRefGoogle Scholar
  3. Bonnett, D.G., Rebetzke, G.J., and Spielmeyer, W., Strategies for efficient implementation of molecular markers in wheat breeding, Mol. Breeding, 2005, vol. 15, pp. 75–78.CrossRefGoogle Scholar
  4. Friebe, B., Jiang, J., Raupp, W.J., et al., Characterization of wheat-alien translocations conferring resistance to diseases and pests: current status, Euphytica, 1996, vol. 91, pp. 59–87.CrossRefGoogle Scholar
  5. Gul’tyaeva, E.I. and Baranova, O.A, Trends in variability of Puccinia triticina population under the influence of cultivated wheat varieties and effectiveness of Lr-genes in the major grain-producing regions of the Russian Federation, in Tekhnologiya sozdaniya i ispol’zovaniya sortov i gibridov s gruppovoi i kompleksnoi ustoichivost’yu k vrednym organizmam v zashchite rastenii (Technology of Creation and Use of Cultivars and Hybrids with Group and Combined Resistance to Pests in Plant Protection), St. Petersburg: RASKhN, Otd. Zashchity Rast., GNU VNIIZR, 2010, pp. 26–48.Google Scholar
  6. Gul’tyaeva, E.I., Metody identifikatsii genov ustoichivosti pshenitsy k buroi rzhavchine s ispol’zovaniem DNK-markerov i kharakteristika effektivnosti Lr-genov (Methods for Identifying Genes for Resistance to Stem Rust of Wheat Using Dna Markers and Characteristics of Efficiency of Lr Genes), St. Petersburg: RASKhN, Otd. Zashchity Rast., GNU VNIIZR, 2012.Google Scholar
  7. Helguera, M., Khan, I.A., Kolmer, J., et al., PCR assays for the Lr37-Yr17-Sr38 cluster of rust resistance genes and their use to develop isogenic hard red spring wheat lines, Crop Sci., 2003, vol. 43, pp. 1839–1847.CrossRefGoogle Scholar
  8. Lagudah, E.S., Mcfadden, H., Singh, R.P., et al., Molecular genetic characterization of the Lr34/Yr18 slow rusting resistance gene region in wheat, Theor. Appl. Genet., 2006, vol. 114, pp. 21–30.PubMedCrossRefGoogle Scholar
  9. Leonova, I.N., Molecular markers: implementation in crop plant breeding for identification, introgression and gene pyramiding, Russ. J. Genet.: Appl. Res., 2013, vol. 3, no. 6, pp. 464–474.CrossRefGoogle Scholar
  10. Mcintosh, R.A., Wellings, C.R., and Park, R.F., Wheat Rust: an Atlas of Resistance Gene, CSIRO, Australia, 1995, pp. 234–237.CrossRefGoogle Scholar
  11. Mcintosh, R.A., Postulation of leaf (brown) rust resistance genes in 70 wheat cultivars grown in United Kingdom, Euphytica, 2001, vol. 120, pp. 205–218.CrossRefGoogle Scholar
  12. McIntosh, R.A., Yamazaki, Y., Dubcovsky, J., et al., Catalogue of Gene Symbols for Wheat, 2010, Suppl. 2011, 2012. Available at http://www.shigen.nig.ac.jp/wheat/komugi/genes/Google Scholar
  13. Nocente, F., Fritz, A.K., Moran, J.L., et al., Identification and molecular tagging of genes Lr1, Lr9, Lr24, Lr47 and their introgression into common wheat cultivars by marker-assisted selection, Euphytica, 2007, vol. 155, pp. 329–336.CrossRefGoogle Scholar
  14. Plaschke, J., Ganal, M.W., and Roder, M.S., Detection of genetic diversity in closely related bread wheat using microsatellite markers, Theor. Appl. Genet., 1995, vol. 91, pp. 1001–1007.PubMedGoogle Scholar
  15. Prins, R., Groenewald, J.Z., Marais, G.F., et al., AFLPSTS tagging of Lr19, a gene conferring resistance to leaf rus tin wheat, Theor. Appl. Genet., 2001, vol. 91, pp. 618–624.CrossRefGoogle Scholar
  16. Prubhu, K.W. and Tiwary, R., Marker assisted breeding in wheat: rust and biotic stresses-I, in ICAR-ACIAR Planning Workshop, Oct. 11–13, 2007, NASC, New Delhi, 2007. http://aciar.gov.au./Files/node/3871/Session% 20IV-Prabhu%20Tiwari.pdfGoogle Scholar
  17. Schachermayer, G., Siedler, H., and Gale, M.D., Identification and localization of molecular markers linked to the Lr9 leaf rust resistance gene of wheat, Theor. Appl. Genet., 1994, vol. 88, pp. 110–115.Google Scholar
  18. Schachermayer, G., Messemer, M., Feuillet, C., et al., Identification of molecular markers linked to the Agropyron elongatum-derived leaf rust resistance gene Lr24 in wheat, Theor. Appl. Genet., 1995, vol. 90, pp. 982–990.Google Scholar
  19. Schachermayer, G., Feuillet, C., and Keller, B., Molecular markers for detection of the wheat leaf rust resistance gene Lr10 in diverse genetic backgrounds, Mol. Breeding, 1997, vol. 3, pp. 65–74.CrossRefGoogle Scholar
  20. Singh, R.P., Huerta-Espino, J., and Wiliam, M., Genetics and breeding for durable resistance to leaf and stripe rusts of wheat, in Increasing Wheat Production in Central Asia through Science and International Cooperation: Proc. 1st Central Asian Wheat Conf., Almaty, Kazakhstan, June 10–13, 2003, Almaty, 2003, pp. 127–132.Google Scholar
  21. Singh, D., Franks, C.D., Huang, L., et al., Lr41, Lr39, and a leaf rust resistance gene from Aegilops cylindrical may be allelic and are located on wheat chromosome 2DS, Theor. Appl. Genet., 2004, vol. 108, pp. 586–591.PubMedCrossRefGoogle Scholar
  22. Sivasamy, M. Vinod, TiwariS., et al., Introgression of useful linked genes for resistance to stem rust, leaf rust and powdery mildew and their molecular validation in wheat (Triticum aestivum L.), Indian J. Genet., 2009, vol. 69, pp. 17–27.Google Scholar
  23. Slikova, S., Gregova, E., and Bartos, P., Development of wheat genotypes possessing a combination of leaf rust resistance genes Lr19 and Lr24, Plant Soil Environ., 2004, vol. 50, no. 10, pp. 434–438.Google Scholar
  24. Sydenham, S.L., Pyramiding wheat rust resistance genes using marker-assisted selection. Master’s theses, University of Free State, Republic of South Africa, 2007. Available at http://etd.uovs.ac.za./ETD-db//theses/available/etd-02052009-140213/iunrestricted/Sydenham S.L.pdfGoogle Scholar
  25. Timonova, E.M., Leonova, I.N., Roder, M.S., and Salina, E.A., Marker-assisted development and characterization of a set of Triticum aestivum lines carrying different introgressions from the T. timopheevii genome, Mol. Breed., 2013, vol. 31, pp. 123–136.CrossRefGoogle Scholar
  26. Vida, G., Gal, M., Uhrin, A., et al., Molecular markers for the identification of resistance genes and markerassisted selection in breeding wheat for leaf rust resistance, Euphytica, 2009, vol. 170, pp. 67–76.CrossRefGoogle Scholar
  27. Weng, Y., et al., PCR-based markers for detection of different sources 1AL.1RS and 1BL.1RS wheat–rye translocation in wheat background, Plant Breed., 2007, vol. 126.Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2015

Authors and Affiliations

  • E. R. Davoyan
    • 1
  • L. A. Bespalova
    • 1
  • R. O. Davoyan
    • 1
  • Yu. S. Zubanova
    • 1
  • D. S. Mikov
    • 1
  • V. A. Filobok
    • 1
  • J. N. Khudokormova
    • 1
  1. 1.Lukyanenko Research Institute of AgricultureRussian Academy of Agricultural SciencesKrasnodarRussia

Personalised recommendations