Application of the electrophoresis of cotyledon storage protein and ISSR-markers to the identification of hybrids between Sorbus sibirica Hedl. and Sorbocotoneaster pozdnjakovii Pojark

  • S. V. Asbaganov
  • E. V. Kobozeva
  • A. V. Agafonov


It is shown that cotyledon protein SDS-electrophoresis and inter-simple sequence repeat DNA markers (ISSRs) can be used in identification of hybrid genotypes in Sorbus sibirica Hedl. and Sorbocotoneaster pozdnjakovii Pojark. Recombination of molecular and morphological characters was observed in hybrids, which proves close relationships of the parental genomes and, as a consequence, the potential of utilizing Sorbocotoneaster pozdnjakovii genotypes in breeding of Sorbus cultivars. The results confirm that the genus Sorbocotoneaster arose as a result of Sorbus and Cotoneaster hybridization.


Sorbocotoneaster mountain ash intergeneric crosses molecular markers 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Agafonov, A.V. and Agafonova, O.V., SDS-PAGE of endosperm proteins in the members of the genus Elymus L. with different genomic structure, Sib. Biol. Zh., 1992, no. 3, pp. 7–12.Google Scholar
  2. Evans, R.C. and Campbell, C.S., The origin of the apple subfamily (Maloideae; Rosaceae) is clarified by DNA sequence data from duplicated GBSSI genes, Am. J. Bot., 2002, vol. 89, no. 9, pp. 1478–1484.CrossRefPubMedGoogle Scholar
  3. Gladkova, V.N., Cytological study of spontaneous hybridogenic genus Sorbocotoneaster Pojark, Botan. Zh., 1967, vol. 52, no. 3, pp. 981–983.Google Scholar
  4. Koropachinskii, I.Yu. and Vstovskaya, T.N., Drevesnye rasteniya Aziatskoi Rossii (Woody Plants of Asian Russia), Novosibirsk: Geo, 2012.Google Scholar
  5. Krasnaya kniga Respubliki Sakha (Yakutii). Redkie i nakhodyashchiesya pod ugrozoi ischeznoveniya vidy rastenii i gribov (The Red Data Book of the Republic of Sakha (Yakutia). Rare and Endangered Species of Plants and Fungi), Yakutsk: NIPK Sakhapoligrafizdat, 2000, pp. 176–177.Google Scholar
  6. Krasnaya kniga Rossiiskoi Federatsii (rasteniya i griby) (The Red Data Book of the Russian Federation (Plants and Fungi)), Moscow: Tovar. Nauch. Izd. KMK, 2008.Google Scholar
  7. Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 1970, vol. 227, no. 5259, pp. 680–685.CrossRefPubMedGoogle Scholar
  8. Liljefors, A., Studies on propagation, embryology and pollination in Sorbus, Acta Horti Bergiani, 1953, vol. 16, pp. 277–329.Google Scholar
  9. Liljefors, A., Cytological studies in Sorbus, Acta Horti Bergiani, 1955, vol. 17, pp. 47–113.Google Scholar
  10. Nei, M. and Li, W.-H., Mathematical model for studying genetic variation in terms of restriction endonucleases, Proc. Natl. Acad. Sci. U.S.A., 1979, vol. 76, no. 10, pp. 5269–5273.CrossRefPubMedCentralPubMedGoogle Scholar
  11. Osborn, T.B., Rastitel’nye belki (Plant Proteins), Moscow: Biomedgiz, 1935.Google Scholar
  12. Peer, Van and Wachter, R.D., TREECON for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment, Comput. Appl. Biosci., 1994, vol. 10, pp. 569–570.PubMedGoogle Scholar
  13. Petrova, A.E., Nazarova, E.I., and Romanova, A.Yu., Introduction of Sorbocotoneaster pozdnjakovii, an endemic of South Yakutia, in Botanicheskie issledovaniya v kriolitozone (Botanical Studies in Permafrost), Yakutsk: YaNTs SO RAN, 1992, pp. 74–80.Google Scholar
  14. Programma i metodika otdalennoi gibridizatsii plodovykh i yagodnykh kul’tur (Program and Technique of Hybridization of Fruit and Berry Crops), Nesterov, Ya.S., Ed., Michurinsk: TSGL im. I.V. Michurina, 1972.Google Scholar
  15. Puchooa, D.A., Simple, rapid and efficient method for the extraction of genomic DNA from lychee (Litchi chinensis Sonn.), Afr. J. April, 2004, vol. 3, no. 4, pp. 253–255.Google Scholar
  16. Redkie i ischezayushchie rasteniya Sibiri (Rare and Endangered Plants of Siberia), Novosibirsk: Nauka, 1980.Google Scholar
  17. Robertson, A., Newton, A.C., and Ennos, R.A., Breeding systems and continuing evolution in the endemic Sorbus taxa on Arran, Heredity, 2004, vol. 93, pp. 487–495.CrossRefPubMedGoogle Scholar
  18. Shcherban’, A.B., Reorganization of plant genome during allopolyploidization, Vavilov Zh. Genet. Selekts., 2013, vol. 17, no. 2, pp. 277–285.Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2015

Authors and Affiliations

  • S. V. Asbaganov
    • 1
  • E. V. Kobozeva
    • 1
  • A. V. Agafonov
    • 1
  1. 1.Central Siberian Botanical Garden SB RASNovosibirskRussia

Personalised recommendations