The influence of particular chromosome regions of Triticum timopheevii on the formation of resistance to diseases and quantitative traits in common wheat

  • E. M. Timonova
  • I. N. Leonova
  • I. A. Belan
  • L. P. Rosseeva
  • E. A. Salina


Evaluation of the influence of Triticum timopheevii Zhuk. introgression fragments (2n = 28, AtAtGG) and their combinations on resistance to leaf and stem rust, powdery mildew, and a number of quantitative traits in 15 introgressive lines of common wheat was conducted. Analysis of introgressive lines by molecular genetic and cytological methods showed the efficiency of using a complex of different types of markers for detailed characterization of hybrid forms and detection of different translocations and substitutions. Evaluation of lines according to their resistance to fungal diseases showed that lines that contain an introgressive fragment of the 5G chromosome in their genome are completely resistant to populations of leaf rust in Western Siberia and the stem rust that is typical for Omsk oblast. Lines 3862-5 and 3862-15, which contain a fragment of the long arm of the 2G chromosome in their genome, were resistant to the population of stem rust in Western Siberia. Introgressive lines were studied for a number of quantitative traits. No negative influence of alien material on the yield and other quantitative traits was observed in all studied lines, which allows one to use them in breeding as donors of resistance to fungal diseases. In addition, the positive influence of T. timopheevii 2G chromosome fragments on the number of grains in an ear was determined.


introgressive lines of common wheat T. timopheevii SSR analysis in situ hybridization C-banding resistance to fungal diseases quantitative traits 


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  1. Badaeva, E.D., Budashkina, E.B., Badaev, N.S., et al., General Features of Chromosome Substitutions in Triticum aestivum × T. timopheevii Hybrids, Theor. Appl. Genet., 1991, vol. 82, pp. 227–232.CrossRefGoogle Scholar
  2. Badaeva, E.D., Badaev, N.S., Gill, B.S., et al., Intraspecific Karyotype Divergence in Triticum araraticum, Plant Syst. Evol., 1994, vol. 192, pp. 117–145.CrossRefGoogle Scholar
  3. Bariana, H.S., Hayden, M.J., Ahmed, N.U., et al., Mapping of Durable Adult Plant and Seedling Resistances to Stripe Rust and Stem Rust Diseases in Wheat, Aust. J. Agric. Res., 2001, vol. 52, pp. 1247–1255.CrossRefGoogle Scholar
  4. Bedbrook, J.R., Jones, J., O’Dell, M., et al., A Molecular Description of Telomeric Heterochromatin in Secale Species, Cell, 1980, vol. 19, pp. 545–560.PubMedCrossRefGoogle Scholar
  5. Brevis, J.C., Chicaiza, O., Khan, I.A., et al., Agronomic and Quality Evaluation of Common Wheat Near-Isogenic Lines Carrying the Leaf Rust Resistance Gene Lr47, Crop Sci., 2008, vol. 48, pp. 1441–1451.CrossRefGoogle Scholar
  6. Brown, J.K.M., Yield Penalties of Disease Resistance in Crops, Curr. Opin. Plant Biol., 2002, vol. 5, pp. 339–344.PubMedCrossRefGoogle Scholar
  7. Budashkina, E.B. and Kalinina, N.P., Development and Genetic Analysis of Common Wheat Introgressive Lines Resistant to Leaf Rust, Acta Phytopathol. Entomol., 2001, vol. 36, pp. 61–65.CrossRefGoogle Scholar
  8. Dyck, P.L. and Friebe, B., Evaluation of Leaf Rust Resistance from Wheat Chromosomal Translocation Lines, Crop Sci., 1993, vol. 33, pp. 687–690.CrossRefGoogle Scholar
  9. Egorova, E., Leonova, I., Budashkina, E., et al., Application of Marker Assisted Selection for Transferring Resistance Genes from Triticum timopheevii to Bread Wheat, in Proc. of the 20th Intern. Conf. on ITMI/2ndWGCJoint Workshop, September 1–5, 2010, Beijing, China, 2010, p. 78.Google Scholar
  10. Friebe, B., Yiang, J., Raupp, W.J., et al., Characterization of Wheat-Alien Translocations Conferring Resistance to Diseases and Pests: Current Status, Euphytica, 1996, vol. 91, pp. 59–87.CrossRefGoogle Scholar
  11. Ganal, M.W. and Röder, M.S., Microsatellite and SNP Markers in Wheat Breeding, in Genomics-Assisted Crop Improvement, Varshney, R.K. and Tuberosa, R., Eds., New York: Springer, 2007, p. 124.Google Scholar
  12. Hayden, M., Good, G., and Sharp, P.J., Sequence Tagged Microsatellite Profiling (STMP): Improved Isolation of DNA Sequence Flanking Target SSRs, Nucleic Acids Res., 2002, vol. 30, pp. 129–133.CrossRefGoogle Scholar
  13. Jarve, K., Peusha, H.O., Tsymbalova, J., et al., Chromosomal Location of a Triticum timopheevii-Derived Powdery Mildew Resistance Gene Transferred to Common Wheat, Genome, 2000, vol. 43, pp. 377–381.PubMedGoogle Scholar
  14. Ji, J.H., Qin, B., Wang, H.Y., et al., STS Markers for Powdery Mildew Resistance Gene Pm6 in Wheat, Euphytica, 2008, vol. 163, pp. 159–165.CrossRefGoogle Scholar
  15. Jiang, J. and Gill, B.S., Sequential Chromosome Banding and in situ Hybridization Analysis, Genome, 1993, vol. 36, pp. 792–795.PubMedCrossRefGoogle Scholar
  16. Jiang, J. and Gill, B.S., Different Species-Specific Chromosome Translocations in Triticum timopheevii and T. turgidum Support the Diphyletic Origin of Polyploidy Wheats, Chrom. Res., 1994, vol. 2, pp. 59–64.PubMedCrossRefGoogle Scholar
  17. Jin, Y. and Singh, R.P., Resistance in US Wheat to Recent Eastern African Isolates of Puccinia graminis f. sp. tritici with Virulence to Resistance Gene Sr31, Plant Dis., 2006, vol. 90, pp. 476–480.CrossRefGoogle Scholar
  18. Jin, Y. and Szabo, L.J., Detection of Virulence to Resistance Gene Sr36 within the TTKS Race Lineage of Puccinia graminis f. sp. tritici, Plant Dis., 2009, vol. 93, no. 4, pp. 367–370.CrossRefGoogle Scholar
  19. Jorgensen, J.H. and Jensen, C.J., Gene Pm6 for Resistance to Powdery Mildew in Wheat, Euphytica, 1973, vol. 22, pp. 4–23.Google Scholar
  20. Kjær, B., Jensen, H.P., Jensen, J., et al., Associations between Three Mlo Powdery Mildew Resistance Genes and Agronomic Traits in Barley, Euphytica, 1990, vol. 46, pp. 185–193.CrossRefGoogle Scholar
  21. Knott, D.R., Translocations Involving Triticum Chromosomes and Agropyron Chromosomes Carrying Leaf Rust Resistance, Can. J. Genet. Cytol., 1968, vol. 10, pp. 695–696.Google Scholar
  22. Kumlay, A.M., Baenziger, P.S., Gill, K.S., et al., Understanding the Effect of Rye Chromatin in Bread Wheat, Crop Sci., 2003, vol. 43, no. 5, pp. 1643–1651.CrossRefGoogle Scholar
  23. Labuschagne, M.T., Pretorius, Z.A., and Grobbelaar, B., The Influence of Leaf Rust Resistance Genes Lr29, Lr34, Lr35 and Lr37 on Bread-Making Quality in Wheat, Euphytica, 2002, vol. 124, pp. 65–70.CrossRefGoogle Scholar
  24. Leonova, I.N., Roder, M.S., Budashkina, E.B., et al., Molecular Analysis of Leaf-Rust-Resistant Introgression Lines Obtained by Crossing of Hexaploid Wheat Triticum aestivum with Tetraploid Wheat Triticum timopheevii, Russ. J. Genet., 2002, vol. 38, no. 12, pp. 1397–1403.CrossRefGoogle Scholar
  25. Leonova, I.N., Roder, M.S., Kalinina, N.P., et al., Genetic Analysis and Localization of Loci Controlling Leaf Rust Resistance of Triticum aestivum × Triticum timopheevii Introgression Lines, Russ. J. Genet., 2008, vol. 44, no. 12, pp. 1431–1437.CrossRefGoogle Scholar
  26. Leonova, I.N., Budashkina, E.B., Flath, K., et al., Microsatellite Mapping of a Leaf Rust Resistance Gene Transferred to Common Wheat from Triticum timopheevii, Cereal Res. Commun., 2010, vol. 38, pp. 212–219.CrossRefGoogle Scholar
  27. Liu, Y., Liu, D., Zhang, H., et al., Allelic Variation, Sequence Determination and Microsatellite Screening at the XGWM261 Locus in Chinese Hexaploid Wheat (Triticum aestivum) Varieties, Euphytica, 2005, vol. 145, pp. 103–112.CrossRefGoogle Scholar
  28. Maghirang, E.B., Lookhart, G.L., Bean, S.R., et al., Comparison of Quality Characteristics and Bread-Making Functionality of Hard Red Winter and Hard Red Spring Wheat, Cereal Chem., 2006, vol. 83, pp. 520–528.CrossRefGoogle Scholar
  29. Mains, E.B. and Jackson, H.S., Physiological Specialization in the Leaf Rust of Wheat, Puccinia triticina Erikss., Phytopathology, 1926, vol. 16, pp. 89–120.Google Scholar
  30. Maxwell, J.J., Lyerly, J.H., Cowger, C., et al., MlAG12: A Triticum timopheevii Derived Powdery Mildew Resistance Gene in Common Wheat on Chromosome 7AL, Theor. Appl. Genet., 2009, vol. 119, pp. 1489–1495.PubMedCrossRefGoogle Scholar
  31. McIntosh, R.A., Yamazaki, Y., Dubcovsky, J., et al., Catalogue of Gene Symbols for Wheat, 2008.
  32. McIntosh, R.A., Wellings, C.R., and Park, R.F., Wheat Rusts: An Atlas of Resistance Genes, Collingwood, Australia: CSIRO Publ., 1995.Google Scholar
  33. Mebrate, S.A., Oerke, E.C., Dehne, H.W., et al., Mapping of the Leaf Rust Resistance Gene Lr38on Wheat Chromosome Arm 6DL using SSR Markers, Euphytica, 2008, vol. 162, pp. 457–466.CrossRefGoogle Scholar
  34. Ortelli, S., Winzeler, H., Winzeler, M., et al., Leaf Rust Resistance Gene Lr9 and Winter Wheat Yield Reduction: I. Yield and Yield Components, Crop Sci., 1996, vol. 36, pp. 1590–1595.CrossRefGoogle Scholar
  35. Perugini, L.D., Murphy, J.P., Marshall, D., et al., Pm37, a New Broadly Effective Powdery Mildew Resistance Gene from Triticum timopheevii, Theor. Appl. Genet., 2008, vol. 116, pp. 417–425.PubMedCrossRefGoogle Scholar
  36. Peterson, R.F., Campbell, A.B., and Hannah, A.E., A Diagrammatic Scale for Estimating Rust Intensity on Leaves and Stems of Cereals, Can. J. Res. (Section C), 1948, vol. 26, pp. 496–500.CrossRefGoogle Scholar
  37. Plaschke, J. and Ganal, M.W., Detection of Genetic Diversity in Closely Related Bread Wheat Using Microsatellite Markers, Theor. Appl. Genet., 1995, vol. 91, pp. 1001–1007.CrossRefGoogle Scholar
  38. Qi, L., Friebe, B., Zhang, P., et al., Homoeologous Recombination, Chromosome Engineering and Crop Improvement, Chromosome Res., 2007, vol. 15, pp. 3–19.PubMedCrossRefGoogle Scholar
  39. Roelfs, A.P., Singh, R.P., and Saari, E.E., Rust Diseases of Wheat: Concepts and Methods of Disease Management, Mexico: CIMMYT, 1992, p. 45.Google Scholar
  40. Saari, E.E. and Prescott, J.M., A Scale for Appraising the Foliar Intensity of Wheat Diseases, Plant Dis. Rep., 1975, vol. 59, pp. 377–380.Google Scholar
  41. Salina, E.A., Egorova, E.M., Adonina, I.G., et al., DNA Markers for Genotyping the Common Wheat (Triticum aestivum L.) Lines with the Genetic Material of Aegilops speltoides Tausch and Triticum timopheevii Zhuk., Inform. Vestnik VOGiS, 2008, vol. 12, no. 4, pp. 620–628.Google Scholar
  42. Salina, E.A., Leonova, I.N., Efremova, T.T., et al., Wheat Genome Structure: Translocations during the Course of Polyploidization, Funct. Integr. Genomics, 2006a, vol. 6, pp. 71–80.PubMedCrossRefGoogle Scholar
  43. Salina, E.A., Lim, K.Y., Badaeva, E.D., et al., Phylogenetic Reconstruction of Aegilops Section sitopsis and the Evolution of Tandem Repeats in the Diploids and Derived Wheat Polyploids, Genome, 2006b, vol. 49, pp. 1023–1035.PubMedCrossRefGoogle Scholar
  44. Singh, R.P., Huerta-Espino, J., Rajaram, S., et al., Agronomic Effects from Chromosome Translocations 7DL.7Ag and 1BL.1RS in Spring Wheat, Crop Sci., 1998, vol. 38, pp. 27–33.CrossRefGoogle Scholar
  45. Sourdille, P., Singh, S., Cadalen, T., et al., Microsatellite-Based Deletion Bin System for the Establishment of Genetic-Physical Map Relationships in Wheat (Triticum aestivum L.), Funct. Integr. Genomics, 2004, vol. 4, pp. 12–25.PubMedCrossRefGoogle Scholar
  46. Tao, W., Liu, D., Liu, J., et al., Genetic Mapping of the Powdery Mildew Resistance Gene Pm6 in Wheat by RFLP Analysis, Theor. Appl. Genet., 2000, vol. 100, pp. 564–556.CrossRefGoogle Scholar
  47. Tsilo, T.J., Jin, Y., and Anderson, J.A., Diagnostic Microsatellite Markers for the Detection of Stem Rust Resistance Gene Sr36 in Diverse Genetic Backgrounds of Wheat, Crop Sci., 2008, vol. 48, pp. 253–261.CrossRefGoogle Scholar
  48. Uhrin, A., Láng, L., and Bed, Z., Comparison of PCR-Based DNA Markers for Using Different Lr19 and Lr24 Leaf Rust Resistance Wheat Sources, Cereal Res. Commun., 2008, vol. 36, no. 4, pp. 533–541.CrossRefGoogle Scholar
  49. Yamamori, M., An N-Band Marker for Gene Lr18 for Resistance to Leaf Rust in Wheat, Theor. Appl. Genet., 1994, vol. 89, pp. 643–646.Google Scholar
  50. Zeven, A.C., Knott, D.R., and Johnson, R., Investigation of Linkage Drag in Near Isogenic Lines of Wheat by Testing for Seedling Reaction to Races of Stem Rust, Leaf Rust and Yellow Rust, Euphytica, 1983, vol. 32, pp. 319–327.CrossRefGoogle Scholar
  51. Zhang, L.Y., Bernard, M., Leroy, P., et al., High Transferability of Bread Wheat EST-Derived SSRs to Other Cereals, Theor. Appl. Genet., 2005, vol. 111, pp. 677–687.PubMedCrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2012

Authors and Affiliations

  • E. M. Timonova
    • 1
  • I. N. Leonova
    • 1
  • I. A. Belan
    • 2
  • L. P. Rosseeva
    • 2
  • E. A. Salina
    • 1
  1. 1.Institute of Cytology and Genetics, Siberian BranchRussian Academy of SciencesNovosibirskRussia
  2. 2.Siberian Agricultural Research Institute, Siberian BranchRussian Academy of Agricultural SciencesOmskRussia

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