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Genetic description of root nodule bacteria of Lathyrus species growing on the territory of the Republic of Bashkortostan

  • An. K. Baymiev
  • K. G. Ptitsyn
  • A. A. Muldashev
  • Al. K. Baymiev
Article

Abstract

The genetic diversity and phylogeny of rhizobia isolated from nodules of nine wild-growing Lathyrus L. species (Fabaceae) growing in the Republic of Bashkortostan were studied. It is shown that, for the given plants, a large variety of heterogeneous strains of root nodule bacteria is characteristic. Nevertheless, it is revealed that the majority of them, in terms of phylogenetics, are closely related to Rhizobium leguminosarum. However, some plant species are found also: nodule bacteria, which were earlier considered unusual for Lathyrus. Thus, L. vernus L. Bernh. and L. sylvestris L. are found to have a root nodule bacteria close to R. tropici, L. palustris L. Agrobacterium sp., and L. gmelinii Fritsch, all bacteria isolated by us from root nodules by the sequence of genes of 16S rRNA, are closely related to Phyllobacterium myrsinacearum.

Keywords

rhizobia nodule bacteria legumes Lathyrus phylogeny genotypic diversity 

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References

  1. Alekseev, Yu.E., Galeva, A.Kh., Gubanov, I.A., et al., Opredelitel’ vysshikh rastenii Bashkirskoi ASSR (An Identification Guide to Higher Plants of the Bashkir ASSR), Moscow: Nauka, 1989.Google Scholar
  2. Asmussen, C. and Liston, A., Chloroplast DNA Characters, Phylogeny and Classification of Lathyrus (Fabaceae), Am. J. Bot., 1998, vol. 85, pp. 387–401.PubMedCrossRefGoogle Scholar
  3. Baymiev, An.Kh., Ptitsyn, K.G., Blagova, D.K., et al., Genetic Diversity and Phylogeny of Root Nodule Bacteria Entering into Symbiosis with Bitter Peavine Lathyrus vernus (L.) Bernh., Microbiology, 2011, vol. 80, no. 1, pp. 96–100.CrossRefGoogle Scholar
  4. Brosius, J., Dull, T.J., Sleeter, D.D., and Noller, H.F., Gene Organization and Primary Structure of a Ribosomal RNA Operon from Escherichia coli, J. Mol. Biol., 1981, vol. 148, pp. 107–127.PubMedCrossRefGoogle Scholar
  5. Kupicha, F.K., The Infrageneric Structure of Lathyrus L., Notes from the Royal Botanic Garden, 1983, vol. 41, no. 2, pp. 209–244.Google Scholar
  6. Kupicha, F.K., The Infrageneric Structure of Vicia, Notes from the Royal Botanic Garden, 1976, vol. 34, no. 3, pp. 287–326.Google Scholar
  7. Mantelin, S., Saux, M.F., Zakhia, F., et al., Emended Description of Thegenus phyllobacterium and Description of Four Novel Species Associated with Plant Roots: Phyllobacterium bourgognense sp. nov., Phyllobacterium ifriqiyense sp. nov., Phyllobacterium leguminum sp. nov. and Phyllobacterium brassicacearum sp. nov., Int. J. Syst. Evol. Microbiol., 2006, vol. 56, pp. 827–839.PubMedCrossRefGoogle Scholar
  8. Martínez, E., Palacios, R., and Sánchez, F., Nitrogenfixing Nodules Induced by Agrobacterium tumefaciens Harboring Rhizobium phaseoli Plasmids, J. Bacteriol., 1987, vol. 169, no. 6, pp. 2828–2834.PubMedGoogle Scholar
  9. Martínez-Romero, E., Segovia, L., Mercante, F.M., et al., Rhizobium tropici, a Novel Species Nodulating Phaseolus vulgaris L. Beans and Leucaena sp. Trees, Int. J. Syst. Bacteriol., 1991, vol. 41, no. 3, pp. 417–426.PubMedCrossRefGoogle Scholar
  10. Muglia, C.I., Grasso, D.H., and Mario Aguilar, O., Rhizobium tropici Response to Acidity Involves Activation of Glutathione Synthesis, Microbiology, 2007, vol. 153, no. 4, pp. 1286–1296.PubMedCrossRefGoogle Scholar
  11. Pariiskaya, A.N. and Klevenskaya, I.L., Distribution in Nature and Possible Pathways of Evolution of Nitrogen-Fixing Symbiosis, Usp. Mikrobiol., vol. 14, pp. 124–147.Google Scholar
  12. Praktikum po pochvovedeniyu (A Practical Course in Soil Science), Kaurichev, S.I., Eds., Moscow: Kolos, 1973.Google Scholar
  13. Provorov, N.A., Relationship between Taxonomy of Legumes and Specificity of Their Interaction with Rhizobia, Bot. Zh., 1992, vol. 77, no. 8, pp. 21–32.Google Scholar
  14. Serebryakov, I.G., Ekologicheskaya morfologiya rastenii. Zhiznennye formy pokrytosemennykh i khvoinykh (Ecological Morphology of Plants. Life Forms of Angiosperms and Conifers), Moscow: Vysshaya Shkola, 1962.Google Scholar
  15. Sliwka, J., Sobkowiak, S., Lebecka, R., et al., Mating Type, Virulence, Aggressiveness and Metalaxyl Resistance of Isolates of Phytophthora infestans in Poland, Potato Res., 2006, vol. 49, pp. 155–166.CrossRefGoogle Scholar
  16. Sui, X.H., Han, L.L., Wang, E.T., et al., Novel Associations between Rhizobial Populations and Legume Species within the Genera Lathyrus and Oxytropis Grown in the Temperate Region of China, Sci. China Ser. Life Sci., 2009, vol. 52, no. 2, pp. 182–192.CrossRefGoogle Scholar
  17. Sullivan, J.T. and Ronson, C.W., Evolution of Rhizobia by Acquisition of a 500-Kb Symbiosis Island that Integrates into a Phe-tRNA Gene, Proc. Natl. Acad. Sci. USA, 1998, vol. 95, pp. 5145–5149.PubMedCrossRefGoogle Scholar
  18. Tikhonovich, I.A and Provorov, N.A., Simbiozy rastenii i mikroorganizmov (Symbiosis of Plants and Microorganisms), St. Petersburg: Izd. S.-Peterb. Univ., 2009.Google Scholar
  19. Tsui, H.P., Materiae ad Floram Lathyrorum sinensium, Bull. Bot. Res., 1984, vol. 4, pp. 36–60.Google Scholar
  20. Vishnyakova, M.A., Burlyaeva, M.O., Alpat’eva, N.V., and Chesnokov, Yu.V., RAPD Analysis of Specific Polymorphism of the Genus Lathyrus L., Family Fabaceae Lindl., Vestnik VOGiS, 2008, vol. 12, no. 4, pp. 595–607.Google Scholar
  21. Williams, J.G., Kubelik, A.R., Livak, K.J., et al., DNA Polymorphisms Amplified by Arbitrary Primers Are Useful as Genetic Markers, Nucleic Acids Res., 1990, vol. 18, no. 22, pp. 6531–6535.PubMedCrossRefGoogle Scholar
  22. Yakovlev, G.P., Bobovye zemnogo shara (Legumes of the World), Menitskii, Yu.L, Eds., Leningrad: Nauka, 1991.Google Scholar
  23. Young, J.P.W., Downer, H.L., and Eardly, B.D., Phylogeny of the Phototrophic Rhizobium Strain BTAi1 by Polymerase Chain Reaction-Based Sequencing of a 16S rRNA Gene Segment, J. Bacteriol., 1991, vol. 173, pp. 2271–2277.PubMedGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2012

Authors and Affiliations

  • An. K. Baymiev
    • 1
  • K. G. Ptitsyn
    • 1
  • A. A. Muldashev
    • 2
  • Al. K. Baymiev
    • 1
  1. 1.Institute for Biochemistry and Genetics of the Research Center of Ufa of Russian Academy of SciencesUfa, BashkortostanRussia
  2. 2.Institute for Biology of the Research Center of Ufa of Russian Academy of SciencesUfa, BashkortostanRussia

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